Abstract：
:The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of the end1 mutant, vps16, vps18 and vps33 mutants all internalize but do not degrade alpha-factor. In addition, all four mutants are defective for alpha-factor-induced projection formation to the same extent. A more detailed investigation of pheromone response in the end1 mutant reveals that one aspect of the early response (induction of FUS1) is as defective as late responses (cell cycle arrest and projection formation). In contrast, another measure of the early response (induction of STE2) is normal. These data suggest that the biogenesis of a functional vacuole is necessary for optimal response to pheromone.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Dulić V,Riezman Hsubject
Has Abstractpub_date
1990-11-01 00:00:00pages
517-25eissn
0021-9533issn
1477-9137journal_volume
97 ( Pt 3)pub_type
杂志文章abstract：:Nervous system development and function are tightly regulated by metabolic processes, including the metabolism of lipids such as fatty acids. Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic intellectual disabilities. We previously reported that Acsl, the Drosophila ortholog of m...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.195032
更新日期：2016-11-01 00:00:00
abstract：:In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.248815
更新日期：2021-01-08 00:00:00
abstract：:NF-kappaB plays a central role in mediating pathogen and cytokine-stimulated gene transcription. NF-kappaB repressing factor (NRF) has been shown to interact with specific negative regulatory DNA elements (NRE) to mediate transcriptional repression by inhibition of the NF-kappaB activity at certain promoters. mRNA abl...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01129
更新日期：2004-07-15 00:00:00
abstract：:Normal human foreskin keratinocytes (HFKs) and transformed HFKs (FEPE1L-8 cells) generated by the introduction of cloned human papillomavirus type 16 sequences were compared for the expression and function of a family of adhesion receptors termed integrins. Initially, cells were examined in conventional monolayer cult...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:Endothelial cells (ECs) sense shear stress and transduce blood flow information into functional responses that play important roles in vascular homeostasis and pathophysiology. A unique feature of shear-stress-sensing is the involvement of many different types of membrane-bound molecules, including receptors, ion chan...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.119628
更新日期：2013-03-01 00:00:00
abstract：:Meiotic chromosome condensation is a unique process, characterized by dramatic changes in chromosome morphology that are required for the correct progression of pairing, synapsis, recombination and segregation of sister chromatids. We used an antibody that recognizes a ser 10 phosphoepitope on histone H3 to monitor H3...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body by receptor-mediated endocytosis. The accumulation of protein complexes in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03052
更新日期：2006-08-01 00:00:00
abstract：:Transglutaminases (TGases) are enzymes which catalyze cross-link formation between glutamine residues and lysine residues in substrate proteins. In the present study, we report for the first time that a representative enzyme, blood coagulation factor XIIIa (FXIIIa), is capable of mediating adhesion of various cells. W...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:By differential screening of a Xenopus laevis egg cDNA library, we have isolated a 2,111 bp cDNA which corresponds to a maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes a 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members of a new protein...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.098087
更新日期：2012-04-15 00:00:00
abstract：:Extracellular matrix receptors of the integrin family have a dual role in the epidermis, regulating both adhesion and differentiation. Loss of contact with the extracellular matrix causes keratinocytes to become committed to terminal differentiation, and results in a decrease in the ability of the alpha 5 beta 1 integ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.071712
更新日期：2010-12-15 00:00:00
abstract：:The cross-striated muscle from the heart ventricle and the smooth penis retractor muscle of the freshwater snail Lymnaea stagnalis have been investigated by X-ray microanalysis to establish whether lanthanum can cross the plasma membrane, as has been reported by other investigators. Tissues were incubated in 1 mM ioni...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Clathrin-mediated endocytosis requires cargo-specific adaptor proteins that recognize specific receptors and recruit them into coated pits. ARH [also called low-density lipoprotein receptor (LDLR) adaptor protein] serves as an adaptor for LDLR endocytosis in liver. However, ARH is dispensable for LDL uptake by some ot...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
abstract：:The Na(+)/H(+) exchanger NHE1 is involved in intracellular pH homeostasis and cell volume regulation and accumulates with actin in the lamellipodia of fibroblasts. In order to determine the role of NHE1 following epithelial transformation and the acquisition of motile and invasive properties, we studied NHE1 expressio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Proteins destined for the mitochondria travel an intricate pathway through two membranes, each with its own receptors and channels. These proteins interact with receptors via N-terminal presequences that form amphipathic helices. Generally, these helices contain abundant positive charges on one face and hydrophobic re...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00750
更新日期：2003-11-15 00:00:00
abstract：:The number of human genetic disorders shown to be due to defects in membrane trafficking has greatly increased during the past five years. Defects have been identified in components involved in sorting of cargo into transport carriers, vesicle budding and scission, movement of vesicles along cytoskeletal tracks, as we...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.03303
更新日期：2006-12-15 00:00:00
abstract：:During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Myosin-X (Myo10) is an unconventional myosin with MyTH4-FERM domains that is best known for its striking localization to the tips of filopodia and its ability to induce filopodia. Although the head domain of Myo10 enables it to function as an actin-based motor, its tail contains binding sites for several molecules wit...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.023549
更新日期：2011-11-15 00:00:00
abstract：:It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.049346
更新日期：2009-11-01 00:00:00
abstract：:Failure to form proper synapses in mechanosensory hair cells, the sensory cells responsible for hearing and balance, leads to deafness and balance disorders. Ribbons are electron-dense structures that tether synaptic vesicles to the presynaptic zone of mechanosensory hair cells where they are juxtaposed with the post-...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.182592
更新日期：2016-06-01 00:00:00
abstract：:Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:The constitutive (or default) pathway for protein secretion was investigated in two epithelial cells, Madin-Darby canine kidney (MDCK) and human colonic adenocarcinoma (Caco-2), using a bacterial enzyme. The choice of a bacterial protein was based on the requirement to identify a protein devoid of sorting signals. The...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but rep...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.161554
更新日期：2015-04-15 00:00:00
abstract：:The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.091066
更新日期：2012-03-15 00:00:00
abstract：:The molecular mechanisms regulating skeletal muscle regeneration and differentiation are not well understood. We analyzed the expression of connexins (Cxs) 40, 43 and 45 in normal and regenerating tibialis anterior muscle and in primary cultures of differentiating myoblasts in adult and newborn mice, respectively. Cxs...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01553
更新日期：2005-01-01 00:00:00
abstract：:Gp78 (also known as AMFR and RNF45) is an E3 ubiquitin ligase that targets proteins for proteasomal degradation through endoplasmic reticulum (ER)-associated degradation (ERAD). In this study, we showed that gp78-mediated ubiquitylation is initiated in the peripheral ER. Substrate monoubiquitylation and gp78 CUE domai...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.096396
更新日期：2012-04-01 00:00:00
abstract：:Polarity of many cell types is controlled by a protein complex consisting of Bazooka/PAR-3 (Baz), PAR-6 and atypical protein kinase C (aPKC). In Drosophila, the Baz-PAR-6-aPKC complex is required for the control of cell polarity in the follicular epithelium, in ectodermal epithelia and neuroblasts. aPKC is the main si...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.052514
更新日期：2009-10-15 00:00:00
abstract：:Vimentin expression in human mammary epithelial MCF10A cells was examined as a function of their migratory status using an in vitro wound-healing model. Analysis of the trajectories of the cells and their migratory speeds by time lapse-video microscopy revealed that vimentin mRNA and protein expression were exclusivel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
