Abstract：
:The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. Leukocytes adhere preferentially to endothelial cell monolayers rather than to smooth muscle cells, adventitial fibroblasts or serum-coated glass. Arterial and venous endothelium are equally good substrates for leukocyte adhesion, and lymphocytes and granulocytes at the same suspension density adhere to endothelial cells in similar numbers. Adherence is greatest during the first 10 min, and is inversely related to flow rate. Numbers of leukocytes adhering to the cell monolayer are proportional to the initial concentration of leukocytes in suspension, although only 2--10% of the leukocytes adhere. Leukocytes that have adhered to the cell monolayer spread over the cells and then migrate, apparently through intercellular junctions. Adhesion requires Ca2+ or Mg2+ ions, but not plasma protein cofactors. Fewer leukocytes adhere to endothelial cells grown for 48 h in rapidly stirred medium than to cells grown under conventional, static conditions.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Beesley JE,Pearson JD,Carleton JS,Hutchings A,Gordon JLsubject
Has Abstractpub_date
1978-10-01 00:00:00pages
85-101eissn
0021-9533issn
1477-9137journal_volume
33pub_type
杂志文章abstract：:In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NP...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.118000
更新日期：2013-06-15 00:00:00
abstract：:The PX domain, which until recently was an orphan domain, has emerged as the latest member of the phosphoinositide-binding module superfamily. Structural studies have revealed that it has a novel fold and identified key residues that interact with the bound phosphoinositide, enabling some prediction of phosphoinositid...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-03-15 00:00:00
abstract：:Nuclear calcium signalling has been a controversial battlefield for many years and the question of how permeable the nuclear pore complexes (NPCs) are to Ca2+ has been the subject of a particularly hot dispute. Recent data from isolated nuclei suggest that the NPCs are open even after depletion of the Ca2+ store in th...
journal_title：Journal of cell science
pub_type： 评论,杂志文章,评审
doi：10.1242/jcs.01295
更新日期：2004-07-01 00:00:00
abstract：:Chloroquine resistance in the human malaria parasite, Plasmodium falciparum, arises from decreased accumulation of the drug in the ;digestive vacuole' of the parasite, an acidic compartment in which chloroquine exerts its primary toxic effect. It has been proposed that changes in the pH of the digestive vacuole might ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02795
更新日期：2006-03-15 00:00:00
abstract：:The water pipes of elongating plant organs are the result of programmed cell death and are formed by the walls of dead and empty protoxylem elements. These protoxylem elements are passively elongated many times by the surrounding tissue before they are replaced and collapse. Well-known adaptations for this unique task...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00966
更新日期：2004-03-01 00:00:00
abstract：:The Arabidopsis thaliana ASY1 gene is essential for homologous chromosome synapsis. Antibodies specific to Asy1 protein and its homologue BoAsy1 from the related crop species Brassica oleracea have been used to investigate the temporal expression and localization of the protein in both species. Asy1 is initially detec...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00048
更新日期：2002-09-15 00:00:00
abstract：:The cloned, SV40-immortalized mouse macrophage cell line, BAC1.2F5, resembles primary macrophages in its dependence on colony-stimulating factor-1 (CSF-1) for both viability and proliferation. Re-addition of CSF-1 stimulates rapid, transient behavioural changes in starved cells, which are rounded, with diffusely organ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:TGFβ binding to the TGFβ receptor (TβR) activates R-Smad-dependent pathways, such as Smad2/3, and R-Smad-independent pathways, such as ERK1/2. The mechanism of the TGFβ-TβRII-TβRI-Smad2/3 pathway is established; however, it is not known how TGFβ activates ERK1/2. We show here that although TGFβ equally activated Smad2...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.076505
更新日期：2011-01-01 00:00:00
abstract：:Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:Epitectin, the mucin-like glycoprotein defined by the monoclonal antibodies CA1, CA2 and CA3, has been examined by electron microscopy to determine its shape and size. It appears to be a single extended strand with a mean length of about 270 nm. The antibodies CA1 and CA2 appear to bind preferentially to a terminal si...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Rapid endocytosis of lipoprotein receptors involves NPxY signals contained in their cytoplasmic tails. Several proteins, including ARH and Dab2, can bind these sequences, but their importance for endocytosis may vary in different cell types. The lipoprotein receptor megalin is expressed in the visceral endoderm (VE), ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02650
更新日期：2005-11-15 00:00:00
abstract：:Sustained neurotransmission requires the tight coupling of synaptic vesicle (SV) exocytosis and endocytosis. The mechanisms underlying this coupling are poorly understood. We tested the hypothesis that a cGMP-dependent protein kinase (PKG), encoded by the foraging (for) gene in Drosophila melanogaster, is critical for...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.227165
更新日期：2019-04-05 00:00:00
abstract：:The specific phosphatase inhibitor okadaic acid (OA) induced fragmentation of the Golgi apparatus in interphase HeLa cells. Immunoelectron microscopy for galactosyltransferase identified a major Golgi fragment composed of a cluster of vesicles and tubules that was morphologically indistinguishable from the 'Golgi clus...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:In the cytoplasm of chlorenchyma cells in primary Origanum dictamnus leaves, rectangular prism-like crystals occur, which, as shown by pepsin digestion, are proteinaceous. In sections they usually show either a square lattice or striations running parallel to the short or long axis. In both cases the interspacings are...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Cells undergo a variety of physiological processes, including division, migration and differentiation, under the influence of endogenous electrical cues, which are generated physiologically and pathologically in the extracellular and sometimes intracellular spaces. These signals are transduced to regulate cell behavio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.023564
更新日期：2009-12-01 00:00:00
abstract：:Mechanical cues are essential for the regulation of cell and tissue physiology. Hence, it has become an utmost necessity for cell biologists to account for those mechanical parameters when investigating biological processes and they need devices to manipulate cells accordingly. Here, we report a simple mechanical cell...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.234666
更新日期：2020-01-06 00:00:00
abstract：:Sexual reproduction (conjugation) in protozoan ciliates is induced by specific cell-cell interactions between cells of complementary mating types. The ancestral ciliate Blepharisma japonicum has two mating types, I and II. The substances that act as signaling molecules in this extracellular interaction for conjugation...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02359
更新日期：2005-06-15 00:00:00
abstract：:It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global tra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.220327
更新日期：2018-09-03 00:00:00
abstract：:Intracellular pools of the heterotrimeric G-protein α-subunit Gαi3 (encoded by GNAI3) have been shown to promote growth factor signaling, while at the same time inhibiting the activation of JNK and autophagic signaling following nutrient starvation. The precise molecular mechanisms linking Gαi3 to both stress and grow...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.241034
更新日期：2020-06-24 00:00:00
abstract：:Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:Epithelia within tubular organs form and expand lumens. Failure of these processes can result in serious developmental anomalies. Although tight junction assembly is crucial to epithelial polarization, the contribution of specific tight junction proteins to lumenogenesis is undefined. Here, we show that ZO-1 (also kno...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.188185
更新日期：2017-01-01 00:00:00
abstract：:Rothmund-Thomson syndrome (RTS) is a human genetic disorder characterized by genome instability, cancer susceptibility and premature aging. The gene defective in a subset of RTS cases, RECQL4, encodes a member of the RecQ family of DNA helicases. To better define the function of the RECQL4 protein, we have determined ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02556
更新日期：2005-09-15 00:00:00
abstract：:Eukaryotic gene expression can be viewed within a conceptual framework in which regulatory mechanisms are integrated at three hierarchical levels. The first is the sequence level, i.e. the linear organization of transcription units and regulatory sequences. Here, developmentally co-regulated genes seem to be organized...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.00779
更新日期：2003-10-15 00:00:00
abstract：:The Wnt signal transduction pathway regulates various aspects of embryonal development and is involved in cancer formation. Wnts induce the stabilisation of cytosolic (beta)-catenin, which then associates with TCF transcription factors to regulate expression of Wnt-target genes. At various levels the Wnt pathway is su...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Keratin 8 is a type II intermediate filament protein found in simple epithelia. We have introduced a 12 kb DNA fragment of the human K8 locus into the germ line of mice. The transgene, containing 1.1 kb of 5' flanking sequences, 7.7 kb corresponding to the body of the gene and 3.2 kb of 3' flanking sequences, was expr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.011130
更新日期：2007-11-01 00:00:00
abstract：:The ret finger protein (rfp) is a member of the B box zinc finger gene family which possesses a tripartite motif consisting of a RING finger, B box finger, and a coiled-coil domain. Rfp is expressed at specific stages of spermatogenesis and in various adult mouse and human tissues. It becomes oncogenic when the tripar...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response, viral infections, DNA damage responses and apoptosis. To date, 28 HECT E3 ubiquitin ligases have been identifi...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.228072
更新日期：2020-04-07 00:00:00
