Centrioles in the mammary epithelium of the rat.

abstract：:Electrical synapses (gap junctions) rapidly transmit signals between neurons and are composed of connexins. In neurons, connexin36 (Cx36) is the most abundant isoform; however, the mechanisms underlying formation of Cx36-containing electrical synapses are unknown. We focus on homocellular and heterocellular gap juncti...

journal_title：Journal of cell science

authors： Meyer A,Hilgen G,Dorgau B,Sammler EM,Weiler R,Monyer H,Dedek K,Hormuzdi SG

更新日期：2014-03-15 00:00:00

abstract：:Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1-null mice to investigate the role of this isoform in Ca(2+) signaling in urinary bladder smooth muscle. We show that RyR1 is required for depolar...

journal_title：Journal of cell science

authors： Fritz N,Morel JL,Jeyakumar LH,Fleischer S,Allen PD,Mironneau J,Macrez N

更新日期：2007-11-01 00:00:00

abstract：:Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...

journal_title：Journal of cell science

authors： Steichele M,Sauermann LS,König AC,Hauck S,Böttger A

更新日期：2021-01-25 00:00:00

abstract：:We have evaluated a series of laminarin sulphates with different degrees of sulphation (0.3-2.3) as antagonists of basic fibroblast growth factor (bFGF) and as inhibitors of the bFGF-dependent endothelial cell line FBHE. Inhibition of binding of bFGF by the laminarin sulphates increased with increasing degree of sulph...

journal_title：Journal of cell science

authors： Hoffman R,Paper DH,Donaldson J,Alban S,Franz G

更新日期：1995-11-01 00:00:00

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:alphaA-Crystallin (alphaA) is a molecular chaperone expressed preferentially in the lens. alphaA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. alphaA(-/-) mouse lenses are smaller than controls, and lens epithelial cells derive...

journal_title：Journal of cell science

authors： Xi JH,Bai F,Andley UP

更新日期：2003-03-15 00:00:00

abstract：:In Saccharomyces cerevisiae, three classes of sphingolipids contain myo-inositol--inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP)(2)C]. No fission yeast equivalent of Ipt1p, the inositolphosphotransferase that synthesizes M(IP)(2)C from MIP...

journal_title：Journal of cell science

authors： Nakase M,Tani M,Morita T,Kitamoto HK,Kashiwazaki J,Nakamura T,Hosomi A,Tanaka N,Takegawa K

更新日期：2010-05-01 00:00:00

abstract：:NADH:ubiquinone oxidoreductases catalyse the first step within the diverse pathways of mitochondrial NADH oxidation. In addition to the energy-conserving form commonly called complex I, fungi and plants contain much simpler alternative NADH:ubiquinone oxido-reductases that catalyze the same reaction but do not translo...

journal_title：Journal of cell science

authors： Kerscher SJ,Okun JG,Brandt U

更新日期：1999-07-01 00:00:00

abstract：:Chloride intracellular channel 3 (CLIC3) drives invasiveness of pancreatic and ovarian cancer by acting in concert with Rab25 to regulate the recycling of α5β1 integrin from late endosomes to the plasma membrane. Here, we show that in two estrogen receptor (ER)-negative breast cancer cell lines, CLIC3 has little influ...

journal_title：Journal of cell science

authors： Macpherson IR,Rainero E,Mitchell LE,van den Berghe PV,Speirs C,Dozynkiewicz MA,Chaudhary S,Kalna G,Edwards J,Timpson P,Norman JC

更新日期：2014-09-15 00:00:00

abstract：:Here, we identify a role for the matrilin-2 (Matn2) extracellular matrix protein in controlling the early stages of myogenic differentiation. We observed Matn2 deposition around proliferating, differentiating and fusing myoblasts in culture and during muscle regeneration in vivo. Silencing of Matn2 delayed the express...

journal_title：Journal of cell science

authors： Deák F,Mátés L,Korpos E,Zvara A,Szénási T,Kiricsi M,Mendler L,Keller-Pintér A,Ozsvári B,Juhász H,Sorokin L,Dux L,Mermod N,Puskás LG,Kiss I

更新日期：2014-08-01 00:00:00

abstract：:Fission yeast cells tolerate the total absence of the cdc25 mitotic inducer in two cases, either in cdc2-3w or in wee1 genetic backgrounds. In the cdc2-3w cdc25Delta double mutant, the rate-limiting step leading to mitosis is reaching a critical size. However, the size control of this mutant operates in late G2, which...

journal_title：Journal of cell science

authors： Sveiczer A,Novak B,Mitchison JM

更新日期：1999-04-01 00:00:00

abstract：:In fibroblasts, platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) stimulate the formation of actin-rich, circular dorsal ruffles (CDRs) and phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of Akt. To test the hypothesis that CDRs increase synthesis of phosphorylated Akt1 (pAkt), we ...

journal_title：Journal of cell science

authors： Yoshida S,Pacitto R,Sesi C,Kotula L,Swanson JA

更新日期：2018-11-16 00:00:00

abstract：:The COP9 signalosome (CSN) is an evolutionarily conserved macromolecular complex that interacts with cullin-RING E3 ligases (CRLs) and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The CSN sequesters inactive CRL4(Ddb2), which rapidly dissociates from the CSN upon DNA damage. Here we systematically ...

journal_title：Journal of cell science

authors： Olma MH,Roy M,Le Bihan T,Sumara I,Maerki S,Larsen B,Quadroni M,Peter M,Tyers M,Pintard L

更新日期：2009-04-01 00:00:00

abstract：:Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...

journal_title：Journal of cell science

authors： Dawe HR,Adams M,Wheway G,Szymanska K,Logan CV,Noegel AA,Gull K,Johnson CA

更新日期：2009-08-01 00:00:00

abstract：:We have examined the pattern of expression of four different matrix metalloproteinases (MMPs), collagenase, stromelysin, 92 kD gelatinase, and 72 kD gelatinase, by primary and passaged cultures of rabbit corneal fibroblasts. Primary cultures of this cell type have previously been shown to reproduce the normal tissue r...

journal_title：Journal of cell science

authors： Fini ME,Girard MT

更新日期：1990-10-01 00:00:00

abstract：:Mammalian homologs of the Drosophila TRP protein have been shown to form cation-permeable channels in the plasma membrane but very little is known about the mechanisms that control their cell surface localization. Recently it has been demonstrated that the last three C-terminal amino acids (TRL) of TRPC4 comprise a PD...

journal_title：Journal of cell science

authors： Mery L,Strauss B,Dufour JF,Krause KH,Hoth M

更新日期：2002-09-01 00:00:00

abstract：:Chloroquine resistance in the human malaria parasite, Plasmodium falciparum, arises from decreased accumulation of the drug in the ;digestive vacuole' of the parasite, an acidic compartment in which chloroquine exerts its primary toxic effect. It has been proposed that changes in the pH of the digestive vacuole might ...

journal_title：Journal of cell science

authors： Hayward R,Saliba KJ,Kirk K

更新日期：2006-03-15 00:00:00

abstract：:The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear A...

journal_title：Journal of cell science

authors： Bobyleva NN,Kudrjavtsev BN,Raikov IB

更新日期：1980-08-01 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:We have previously shown by examining the anchorage dependence, density-dependent inhibition of growth, LETS protein and microfilament bundles that the transformed phenotype of the parental tumours are suppressed in hybrids between rat embryo fibroblast (REF) and mouse tumour cells (TA3B). Hybrids between TA3B and Syr...

journal_title：Journal of cell science

authors： Marshall CJ

更新日期：1979-10-01 00:00:00

abstract：:Certain embryonic tissue masses and cell aggregates behave like deformable solids during brief experimental manipulations but like viscous liquids in long-term organ cultures. To investigate these seemingly paradoxical physical properties, we have mechanically deformed cell aggregates derived from several embryonic ch...

journal_title：Journal of cell science

authors： Phillips HM,Steinberg MS

更新日期：1978-04-01 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor ...

journal_title：Journal of cell science

authors： Prasad S,Ponimaskin E,Zeug A

更新日期：2019-08-23 00:00:00

abstract：:It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...

journal_title：Journal of cell science

authors： Couchman JR,Woods A

更新日期：1999-10-01 00:00:00

abstract：:Rickettsia conorii, the causative agent of Mediterranean spotted fever, is able to attach to and invade a variety of cell types both in vitro and in vivo. Although previous studies show that entry of R. conorii into non-phagocytic cells relies on actin polymerization, little else is known about the molecular details g...

journal_title：Journal of cell science

authors： Martinez JJ,Cossart P

更新日期：2004-10-01 00:00:00

abstract：:Transforming growth factor beta (TGFbeta) plays an important role in development and maintenance of murine yolk sac vascular development. Targeted deletions of Tgfb1 and other components of this signaling pathway, such as Acvrl1, Tgfbr1 and Tgfbr2, result in abnormal vascular development especially of the yolk sac, le...

journal_title：Journal of cell science

authors： Poon E,Clermont F,Firpo MT,Akhurst RJ

更新日期：2006-02-15 00:00:00

abstract：:We investigated mechanisms of the p73alpha-mediated repression of the platelet-derived growth factor beta-receptor (PDGFRB) promoter caused by its interaction with NF-Y. Treatment of cells with the histone deacetylase (HDAC) inhibitor, Trichostatin A, increases PDGFRB promoter activity through the CCAAT motif and coun...

journal_title：Journal of cell science

authors： Uramoto H,Wetterskog D,Hackzell A,Matsumoto Y,Funa K

更新日期：2004-10-15 00:00:00

abstract：:A method is reported for preparing surface (plasma) membranes from adrenal tumour (Y-1) cells. The procedure is based upon homogenization in hypotonic ZnCl2 followed by sedimentation through two sucrose density gradients. The purified membranes consist of large sheets of membrane. The identity and purity of the membra...

journal_title：Journal of cell science

authors： Osawa S,Hall PF

更新日期：1985-08-01 00:00:00

abstract：:Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...

journal_title：Journal of cell science

authors： Zhu J,Zhou K,Hao JJ,Liu J,Smith N,Zhan X

更新日期：2005-02-15 00:00:00

abstract：:Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin...

journal_title：Journal of cell science

authors： Hatano T,Alioto S,Roscioli E,Palani S,Clarke ST,Kamnev A,Hernandez-Fernaud JR,Sivashanmugam L,Chapa-Y-Lazo B,Jones AME,Robinson RC,Sampath K,Mishima M,McAinsh AD,Goode BL,Balasubramanian MK

更新日期：2018-04-23 00:00:00