Abstract:
:Chloroquine resistance in the human malaria parasite, Plasmodium falciparum, arises from decreased accumulation of the drug in the ;digestive vacuole' of the parasite, an acidic compartment in which chloroquine exerts its primary toxic effect. It has been proposed that changes in the pH of the digestive vacuole might underlie the decreased accumulation of chloroquine by chloroquine-resistant parasites. In this study we have investigated the digestive vacuole pH of a chloroquine-sensitive and a chloroquine-resistant strain of P. falciparum, using a range of dextran-linked pH-sensitive fluorescent dyes. The estimated digestive vacuole pH varied with the concentration and pK(a) of the dye, ranging from approximately 3.7-6.5. However, at low dye concentrations the estimated digestive vacuole pH of both the chloroquine-resistant and chloroquine-sensitive strains converged in the range 4.5-4.9. The results suggest that there is no significant difference in digestive vacuole pH of chloroquine-sensitive and chloroquine-resistant parasites, and that digestive vacuole pH does not play a primary role in chloroquine resistance.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Hayward R,Saliba KJ,Kirk Kdoi
10.1242/jcs.02795keywords:
subject
Has Abstractpub_date
2006-03-15 00:00:00pages
1016-25issue
Pt 6eissn
0021-9533issn
1477-9137pii
jcs.02795journal_volume
119pub_type
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