Abstract：
:Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tight junctions and desmosomes were lesion-free. Compared with day 1 of pregnancy, lesions on the apical plasma membrane were much more extensive and some were of different morphology on day 6 - the day of blastocyst implantation. We consider mechanisms of lesion formation and interpret the results to indicate a higher content and perhaps a different organization of cholesterol in the apical plasma membrane on day 6 of pregnancy. We also suggest how this increase may occur.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Murphy CR,Martin Bsubject
Has Abstractpub_date
1985-10-01 00:00:00pages
163-72eissn
0021-9533issn
1477-9137journal_volume
78pub_type
杂志文章abstract：:Non-coding RNAs (ncRNAs) are nucleotide sequences that are known to assume regulatory roles previously thought to be reserved for proteins. Their functions include the regulation of protein activity and localization and the organization of subcellular structures. Sequencing studies have now identified thousands of ncR...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.246744
更新日期：2020-11-05 00:00:00
abstract：:In the presence of sialic acid donors Trypanosoma cruzi acquires up to 10(7) sialic acid residues on its surface, in a reaction catalyzed by its unique trans-sialidase. Most of these sialic acid residues are incorporated into mucin-like glycoproteins. To further understand the biological role of parasite sialylation, ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.187112
更新日期：2016-09-01 00:00:00
abstract：:Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.059634
更新日期：2010-01-15 00:00:00
abstract：:Actin functions in a multitude of cellular processes owing to its ability to polymerize into filaments, which can be further organized into higher-order structures by an array of actin-binding and regulatory proteins. Therefore, research on actin and actin-related functions relies on the visualization of actin structu...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.189068
更新日期：2017-02-01 00:00:00
abstract：:Integrin alpha1beta1 is a collagen receptor predominantly found in mesenchymal tissues. Mice lacking this receptor are viable. We have previously suggested that alpha1beta1 might participate in the down-regulation of collagen gene expression observed in cells suspended inside collagen gels. The results presented here ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:The coordinated trafficking and tethering of membrane cargo within cells relies on the function of distinct cytoskeletal motors that are targeted to specific subcellular compartments through interactions with protein adaptors and phospholipids. The unique actin motor myosin VI functions at distinct steps during clathr...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.095554
更新日期：2013-06-15 00:00:00
abstract：:Prion diseases are fatal transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrP(C)) denoted PrP(Sc). Recently, wild-type and pathogenic PrP mutants have been shown to be degraded by the endoplasmic reticulum-associated degradation ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01094
更新日期：2004-05-01 00:00:00
abstract：:The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:We have identified a novel mammalian protein, MIR1, with microtubule-binding activity. MIR1 is a relative of MID1/midin, the protein implicated in Opitz G/BBB syndrome. In tissue culture cells, MIR1 is enriched at the centrosome. MIR1 dissociates from centrosomes at the G2/M transition and is recruited back to spindle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The forked protein is an actin binding protein involved in the formation of large actin fiber bundles in developing Drosophila bristles. These are the largest example of a type of actin bundle characterized by parallel, hexagonally packed actin fibers, also found in intestinal microvilli, kidney proximal tubule microv...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Circadian rhythms regulate a wide variety of physiological and metabolic processes. The clock machinery comprises complex transcriptional-translational feedback loops that, through the action of specific transcription factors, modulate the expression of as many as 10% of cellular transcripts. This marked change in gen...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.051649
更新日期：2010-11-15 00:00:00
abstract：:Casein kinase 1 (CK1) plays central roles in various signal transduction pathways and performs many cellular activities. For many years CK1 was thought to act independently of modulatory subunits and in a constitutive manner. Recently, DEAD box RNA helicases, in particular DEAD box RNA helicase 3 X-linked (DDX3X), wer...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.207316
更新日期：2018-01-04 00:00:00
abstract：:Inhibitory and excitatory neurons exhibit distinct patterns of development in the mammalian cerebral cortex. The morphological development of inhibitory and excitatory neurons derived from fetal rat cerebral cortex has now been compared in vitro. Inhibitory neurons were identified by immunofluorescence staining with a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00762
更新日期：2003-11-01 00:00:00
abstract：:Diploid yeast, like most eukaryotes, can undergo meiotic differentiation to form haploid gametes. Meiotic differentiation and cell growth (proliferation) are mutually exclusive programs, and in yeast the switch between growth and meiosis is controlled by nutritional signals. The signaling pathways that mediate nutriti...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.00460
更新日期：2003-06-01 00:00:00
abstract：:The regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.172429
更新日期：2015-11-15 00:00:00
abstract：:Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.235390
更新日期：2019-12-13 00:00:00
abstract：:Entry into mitosis requires p34(cdc2), which activates downstream mitotic events through phosphorylation of key target proteins. In Aspergillus nidulans, the NIMA protein kinase has been identified as a potential downstream target and plays a role in regulating chromatin condensation at mitosis. nimA- mutants arrest i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.022103
更新日期：2008-04-01 00:00:00
abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.206417
更新日期：2018-05-10 00:00:00
abstract：:Transforming growth factor beta (TGFbeta) plays an important role in development and maintenance of murine yolk sac vascular development. Targeted deletions of Tgfb1 and other components of this signaling pathway, such as Acvrl1, Tgfbr1 and Tgfbr2, result in abnormal vascular development especially of the yolk sac, le...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02788
更新日期：2006-02-15 00:00:00
abstract：:The Tec kinases represent the second largest family of mammalian non-receptor tyrosine kinases and are distinguished by the presence of distinct proline-rich regions and pleckstrin homology domains that are required for proper regulation and activation. Best studied in lymphocyte and mast cells, these kinases are crit...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-08-01 00:00:00
abstract：:We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.196790
更新日期：2017-03-15 00:00:00
abstract：:Normal human foreskin keratinocytes (HFKs) and transformed HFKs (FEPE1L-8 cells) generated by the introduction of cloned human papillomavirus type 16 sequences were compared for the expression and function of a family of adhesion receptors termed integrins. Initially, cells were examined in conventional monolayer cult...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.216259
更新日期：2018-08-03 00:00:00
abstract：:Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03170
更新日期：2006-10-15 00:00:00
abstract：:The specific phosphatase inhibitor okadaic acid (OA) induced fragmentation of the Golgi apparatus in interphase HeLa cells. Immunoelectron microscopy for galactosyltransferase identified a major Golgi fragment composed of a cluster of vesicles and tubules that was morphologically indistinguishable from the 'Golgi clus...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Microinjected GTP gamma S revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII dependent export of ts-O45-G from the ER is blocked in cells injected with recombinant p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Interstitial stem cells in Hydra are rapidly proliferating multipotent stem cells which continuously give rise to precursors for nerve and nematocyte differentiation. Growth of the stem cell population is controlled by the cell cycle time of the stem cells and the self-renewal probability, Ps (the fraction of stem cel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
