Scrapie-like prion protein is translocated to the nuclei of infected cells independently of proteasome inhibition and interacts with chromatin.

abstract：:The Down syndrome critical region (DSCR) on Chromosome 21 contains many genes whose duplication may lead to the major phenotypic features of Down syndrome and especially the associated mental retardation. However, the functions of DSCR genes are mostly unknown and their possible involvement in key brain developmental ...

journal_title：Journal of cell science

authors： Berto G,Camera P,Fusco C,Imarisio S,Ambrogio C,Chiarle R,Silengo L,Di Cunto F

更新日期：2007-06-01 00:00:00

abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...

journal_title：Journal of cell science

authors： Casagolda D,Del Valle-Pérez B,Valls G,Lugilde E,Vinyoles M,Casado-Vela J,Solanas G,Batlle E,Reynolds AB,Casal JI,de Herreros AG,Duñach M

更新日期：2010-08-01 00:00:00

abstract：:The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...

journal_title：Journal of cell science

authors： Danielpour D

更新日期：1999-01-01 00:00:00

abstract：:In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...

journal_title：Journal of cell science

authors： Sprocati T,Ronchi P,Raimondi A,Francolini M,Borgese N

更新日期：2006-08-01 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP1) is a nuclear enzyme that can trigger caspase-independent necrosis. Two main mechanisms for this have been proposed: one involving RIP1 and JNK kinases and mitochondrial permeability transition (MPT), the other involving calpain-mediated activation of Bax and mitochondrial release ...

journal_title：Journal of cell science

authors： Douglas DL,Baines CP

更新日期：2014-10-01 00:00:00

abstract：:Double minutes (DMs) seen in a substantial fraction of human tumors are the cytogenetic manifestation of gene amplification which renders the tumor cells advantageous for growth and survival. DMs are acentric and atelomeric chromatin composed of circular DNA. In this study, we found they showed a remarkable relocation...

journal_title：Journal of cell science

authors： Itoh N,Shimizu N

更新日期：1998-11-01 00:00:00

abstract：:Four developmental stages of Rana pipiens, R. pipiens female X R. catesbeiana female, and R. pipiens female X R. esculenta male embryos were fixed in standard electron microscopic fixatives with or without 1% lanthanum nitrate (LN) added. Embryos fixed without LN were embedded and sectioned at 0-5 micrometer and stain...

journal_title：Journal of cell science

authors： Johnson KE

更新日期：1977-06-01 00:00:00

abstract：:The alpha6beta4 integrin is located at the basal surface of keratinocytes, in hemidesmosomal structures that mediate stable adhesion of epidermal cells to the underlying basement membrane component laminin-5. The absence of alpha6beta4 integrin causes junctional epidermolysis bullosa, a severe blistering disease of th...

journal_title：Journal of cell science

authors： Raymond K,Kreft M,Janssen H,Calafat J,Sonnenberg A

更新日期：2005-03-01 00:00:00

abstract：:We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...

journal_title：Journal of cell science

authors： Millar SE,Freeman M,Glover DM

更新日期：1987-02-01 00:00:00

abstract：:Sister-chromatid arm cohesion is lost during the metaphase I/anaphase I transition to allow homologue separation. To obtain needed information on this process we have analysed in grasshopper bivalents the sequential release of arm cohesion in relation to the behaviour of chromatid axes. Results show that sister axes a...

journal_title：Journal of cell science

authors： Suja JA,Antonio C,Debec A,Rufas JS

更新日期：1999-09-01 00:00:00

abstract：:Cellular lipids play crucial roles in the cell, including in energy storage, the formation of cellular membranes, and in signaling and vesicular trafficking. To understand the functions and characteristics of lipids within cells, various methods to image lipids have been established. In this Commentary, we discuss the...

journal_title：Journal of cell science

authors： Maekawa M,Fairn GD

更新日期：2014-11-15 00:00:00

abstract：:Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist in two subpopulations, large precursors at the cell periphery and smaller podosomes (successors) in the cell interior. To date, the mechanisms that differentially regulate these subpopulations are largely unknown. Her...

journal_title：Journal of cell science

authors： Bhuwania R,Cornfine S,Fang Z,Krüger M,Luna EJ,Linder S

更新日期：2012-05-01 00:00:00

abstract：:Non-coding RNAs (ncRNAs) are nucleotide sequences that are known to assume regulatory roles previously thought to be reserved for proteins. Their functions include the regulation of protein activity and localization and the organization of subcellular structures. Sequencing studies have now identified thousands of ncR...

journal_title：Journal of cell science

authors： Bensaoud C,Martins LA,Aounallah H,Hackenberg M,Kotsyfakis M

更新日期：2020-11-05 00:00:00

abstract：:During growth, homeostasis and regeneration, stem cells are exposed to different energy demands. Here, we characterise the metabolic pathways that mediate the commitment and differentiation of mouse skeletal muscle stem cells, and how their modulation can influence the cell state. We show that quiescent satellite stem...

journal_title：Journal of cell science

authors： Pala F,Di Girolamo D,Mella S,Yennek S,Chatre L,Ricchetti M,Tajbakhsh S

更新日期：2018-07-27 00:00:00

abstract：:Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, the underlying mechanisms are still poorly understood. To address this issue, we assessed the effect of fibroblast-derived diffusible factors on the growth and morph...

journal_title：Journal of cell science

authors： Soriano JV,Pepper MS,Nakamura T,Orci L,Montesano R

更新日期：1995-02-01 00:00:00

abstract：:Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...

journal_title：Journal of cell science

authors： Jackman MR,Shurety W,Ellis JA,Luzio JP

更新日期：1994-09-01 00:00:00

abstract：:Tetrahymena thermophila swims by the coordinated beating of hundreds of cilia that cover its body. It has been proposed that the outer arm dyneins of the ciliary axoneme control beat frequency, whereas the inner arm dyneins control waveform. To test the role of one of these inner arms, dynein heavy chain 7 protein (Dy...

journal_title：Journal of cell science

authors： Wood CR,Hard R,Hennessey TM

更新日期：2007-09-01 00:00:00

abstract：:The notion that cell shape and spreading can regulate cell proliferation has evolved over several years, but only recently has this been linked to forces from within and upon the cell. This emerging area of mechanical signaling is proving to be wide-spread and important for all cell types. The microenvironment that su...

journal_title：Journal of cell science

authors： Provenzano PP,Keely PJ

更新日期：2011-04-15 00:00:00

abstract：:The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possi...

journal_title：Journal of cell science

authors： Rabouille C,Hui N,Hunte F,Kieckbusch R,Berger EG,Warren G,Nilsson T

更新日期：1995-04-01 00:00:00

abstract：:Integrins mediate cell adhesion and signal transduction at focal adhesions. Here we investigate the roles of integrin beta subunits in the regulation of actin cytoskeletal structure and the activities of Rho and Rac. The overexpression of beta3 integrin in Chinese hamster ovary cells enhances Rho activity and stress f...

journal_title：Journal of cell science

authors： Miao H,Li S,Hu YL,Yuan S,Zhao Y,Chen BP,Puzon-McLaughlin W,Tarui T,Shyy JY,Takada Y,Usami S,Chien S

更新日期：2002-05-15 00:00:00

abstract：:Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structu...

journal_title：Journal of cell science

authors： Manandhar G,Onishchenko GE

更新日期：1995-02-01 00:00:00

abstract：:The protozoan parasite Giardia intestinalis (also known as Giardia lamblia) is a major waterborne pathogen. During its life cycle, Giardia alternates between the actively growing trophozoite, which has two diploid nuclei with low levels of allelic heterozygosity, and the infectious cyst, which has four nuclei and a to...

journal_title：Journal of cell science

authors： Carpenter ML,Assaf ZJ,Gourguechon S,Cande WZ

更新日期：2012-05-15 00:00:00

abstract：:The conversion of the cellular prion protein (PrPc) into a pathogenic isoform (PrP(Sc)) is one of the underlying events in the pathogenesis of the fatal transmissible spongiform encephalopathies (TSEs). Numerous compounds have been described to inhibit prion replication and PrP(Sc) accumulation in cell culture. Among ...

journal_title：Journal of cell science

authors： Nunziante M,Kehler C,Maas E,Kassack MU,Groschup M,Schätzl HM

更新日期：2005-11-01 00:00:00

abstract：:Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...

journal_title：Journal of cell science

authors： Liu H,Chen SE,Jin B,Carson JA,Niu A,Durham W,Lai JY,Li YP

更新日期：2010-09-01 00:00:00

abstract：:We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

journal_title：Journal of cell science

authors： Coukell MB,Cameron AM,Adames NR

更新日期：1992-10-01 00:00:00

abstract：:The Arabidopsis thaliana ASY1 gene is essential for homologous chromosome synapsis. Antibodies specific to Asy1 protein and its homologue BoAsy1 from the related crop species Brassica oleracea have been used to investigate the temporal expression and localization of the protein in both species. Asy1 is initially detec...

journal_title：Journal of cell science

authors： Armstrong SJ,Caryl AP,Jones GH,Franklin FC

更新日期：2002-09-15 00:00:00

abstract：:Transcription factor IIH (TFIIH) participates in transcription, nucleotide excision repair and the control of the cell cycle. In the present study, we demonstrate that the Dmp52 subunit of TFIIH in Drosophila physically interacts with the fly p53 homologue, Dp53. The depletion of Dmp52 in the wing disc generates chrom...

journal_title：Journal of cell science

authors： Villicaña C,Cruz G,Zurita M

更新日期：2013-06-01 00:00:00

abstract：:An electron microscopic investigation of the events associated with meiosis in Lilium has revealed a number of changes in both the organellar population and the other cytoplasmic components. Ribosome numbers decrease significantly in early prophase and are later replenished in the tetrads, a process most likely involv...

journal_title：Journal of cell science

authors： Bird J,Porter EK,Dickinson HG

更新日期：1983-01-01 00:00:00

abstract：:Fission yeast cells tolerate the total absence of the cdc25 mitotic inducer in two cases, either in cdc2-3w or in wee1 genetic backgrounds. In the cdc2-3w cdc25Delta double mutant, the rate-limiting step leading to mitosis is reaching a critical size. However, the size control of this mutant operates in late G2, which...

journal_title：Journal of cell science

authors： Sveiczer A,Novak B,Mitchison JM

更新日期：1999-04-01 00:00:00

abstract：:Recent studies have shown that Src-family kinases (SFKs) play an important role in mediating integrin signalling, and the beta3 subunit of alphaIIbbeta3 integrin has been shown to interact with multiple SFK members. Here, we analyzed the interactions and functional consequences of Fyn and Src binding to alphaIIbbeta3....

journal_title：Journal of cell science

authors： Reddy KB,Smith DM,Plow EF

更新日期：2008-05-15 00:00:00