Abstract：
:Integrins mediate cell adhesion and signal transduction at focal adhesions. Here we investigate the roles of integrin beta subunits in the regulation of actin cytoskeletal structure and the activities of Rho and Rac. The overexpression of beta3 integrin in Chinese hamster ovary cells enhances Rho activity and stress fiber formation, whereas the overexpression of beta1 integrin increases Rac activity and lamellipodia formation. The overexpression of a mutant beta1-3-1 integrin, in which the extracellular I-domain-like sequence of beta1 integrin has been replaced with the corresponding sequence of beta3 integrin, also enhances Rho activity and the formation of stress fibers. Our results demonstrate that beta1 and beta3 integrins differentially regulate the activities of Rho family GTPases and that the extracellular domains of integrin beta subunits play a critical role in transducing the extracellular ligand-binding information into specific intracellular signaling events.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Miao H,Li S,Hu YL,Yuan S,Zhao Y,Chen BP,Puzon-McLaughlin W,Tarui T,Shyy JY,Takada Y,Usami S,Chien Skeywords:
subject
Has Abstractpub_date
2002-05-15 00:00:00pages
2199-206issue
Pt 10eissn
0021-9533issn
1477-9137journal_volume
115pub_type
杂志文章abstract：:Laminins are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin (Lama3(fl/fl)), one of the main laminin subunits in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.080911
更新日期：2011-09-01 00:00:00
abstract：:In fibroblasts, platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) stimulate the formation of actin-rich, circular dorsal ruffles (CDRs) and phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of Akt. To test the hypothesis that CDRs increase synthesis of phosphorylated Akt1 (pAkt), we ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.220517
更新日期：2018-11-16 00:00:00
abstract：:The marginal band microtubules of isolated Xenopus erythrocyte cytoskeletons possess the stability properties of non-steady-state microtubules. They are unperturbed by low temperatures, a variety of microtubule inhibitors, hypotonic treatment and the direct action of calcium. These microtubules can be rapidly depolyme...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:The Sendai virus-induced fusion of HeLa cells has been studied by freeze-fracture electron microscopy. Freeze-fracture observations confirm previous scanning electron-microscope studies (1977) and show that at 4 degrees C virus particles bind to the cell surface and that cell agglutination results from the crosslinkin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA. Using a double-labelling procedure we have compared, at different salt concentrations, t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:The efficient functioning of striated muscle is dependent upon the structure of several cytoskeletal networks including myofibrils, microtubules, and intermediate filaments. However, little is known about how these networks function together during muscle differentiation and maintenance. In vitro studies suggest that ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01158
更新日期：2004-07-01 00:00:00
abstract：:In a previous study we demonstrated that a homeobox peptide corresponding to the 60 amino acid long DNA-binding region of the Drosophila antennapedia homeo-protein was capable of crossing the plasma membrane of cells in culture. This finding has led us to investigate whether chimeric molecules encompassing the homeobo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:EPHA2 receptor tyrosine kinase is overexpressed in several human cancer types and promotes malignancy. However, the mechanisms by which EPHA2 promotes tumor progression are not completely understood. Here we report that overexpression of a wild-type EPHA2, but not a signaling-defective cytoplasmic truncation mutant (D...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.017145
更新日期：2008-02-01 00:00:00
abstract：:Intercellular propagation of signals through connexin32-containing gap junctions is of major importance in physiological processes like nerve activity-dependent glucose mobilization in liver parenchymal cells and enzyme secretion from pancreatic acinar cells. In these cells, as in other organs, more than one type of c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:To enhance our understanding of the function(s) of gamma-tubulin-complex proteins (GCPs), we identified and analyzed the functions of the Aspergillus nidulans homologs of GCP2-GCP6 (here designated GCPB-GCBF). The gamma-tubulin small complex (gamma-TuSC) components, gamma-tubulin, GCPB and GCPC, are essential for viab...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.059196
更新日期：2009-11-15 00:00:00
abstract：:A defining feature of malignant tumor progression is cellular penetration through the basement membrane and interstitial matrices that separate various cellular compartments. Accumulating evidence supports the notion that invasive cells employ specialized structures termed invadopodia to breach these structural barrie...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.097956
更新日期：2012-02-01 00:00:00
abstract：:The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.148296
更新日期：2015-05-15 00:00:00
abstract：:Actin-based myosin motors have a pivotal role in intracellular trafficking in eukaryotic cells. The parasitic protozoan organism Leishmania expresses a novel class of myosin, myosin XXI (Myo21), which is preferentially localized at the proximal region of the flagellum. However, its function in this organism remains la...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.064725
更新日期：2010-06-15 00:00:00
abstract：:Programmed cell death comprises several subtypes, as revealed by electron microscopy. Apoptosis or type I programmed cell death is characterized by condensation of cytoplasm and preservation of organelles, essentially without autophagic degradation. Autophagic cell death or type II programmed cell death exhibits exten...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Although significant progress has recently been made in culturing mammalian urothelial cells, relatively little is known about their biochemical differentiation. In this paper, we assessed the differentiation state of cultured bovine urothelial cells by analyzing their keratins and a cell surface marker, uroplakin I. ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The contractile vacuole of the chloromonadophycean alga Vacuolaria virescens is a permanent structure that possesses a specialized membrane: subunits of this membrane have a diameter of 21-24 nm and in places are arranged in a regular hexagonal pattern. The lateral walls of these subunits form regularly spaced bristle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear A...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Two of the best understood somatic cell mRNA cytoplasmic trafficking elements are those governing localization of beta-actin and myelin basic protein mRNAs. These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively. It is not known whether these elements fulfil other roles in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00137
更新日期：2002-12-01 00:00:00
abstract：:The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results suppo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Rho GTPases and their downstream effectors regulate changes in the actin cytoskeleton that underlie cell motility and adhesion. They also participate, with RhoA, in the regulation of gene transcription by activating serum response factor (SRF)-mediated transcription from the serum response element (SRE). SRF-mediated ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.013557
更新日期：2008-02-15 00:00:00
abstract：:Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:The cell types responsible for osteolysis associated with skeletal metastasis of solid malignancies are unclear. Tumour infiltrating macrophages (TIMs) isolated from primary mammary carcinomas of C3H/Avy mice were cultured on bone slices to assess their ability to resorb bone. After 14 days in co-culture with murine m...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The weak spindle integrity checkpoint in Drosophila spermatocytes has revealed a novel function of the gamma-tubulin ring complex (gammaTuRC) in maintaining spindle bipolarity throughout meiosis. Bipolar and bi-astral spindles could form in Drosophila mutants for dd4, the gene encoding the 91 kDa subunit of gammaTuRC....
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00295
更新日期：2003-03-01 00:00:00
abstract：:Rac1 and Rac3 are highly homologous regulatory proteins that belong to the small GTPases of the Rho family. Previously, we showed that Rac3 induces cell rounding and prevents neuronal differentiation, in contrast to its close relative Rac1, which stimulates cell spreading and neuritogenesis. To explain these opposing ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.039958
更新日期：2009-06-15 00:00:00
abstract：:We previously showed that the cell-cell junction protein plakoglobin (PG) not only suppresses motility of keratinocytes in contact with each other, but also, unexpectedly, of single cells. Here we show that PG deficiency results in extracellular matrix (ECM)-dependent disruption of mature focal adhesions and cortical ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.070391
更新日期：2010-10-15 00:00:00
abstract：:Many adherent cells in vitro are surrounded by a transparent exclusion zone or halo, several micrometers thick, which red blood cells, bacteria and carbon particles cannot penetrate. This halo is rapidly and specifically removed by hyaluronidase and its high degree of hydration is demonstrated by the fact that, althou...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.221317
更新日期：2018-11-05 00:00:00
abstract：:Using a genome-wide technical knockout, we isolated a newly identified set of GRIM (genes associated with retinoid-interferon-induced mortality) genes; GRIM genes mediate IFN- and retinoic-acid (RA)-induced cell death. Here, we describe the isolation and characterization of one such gene, GRIM-1. Three proteins, with ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.070250
更新日期：2010-08-15 00:00:00
abstract：:We have previously demonstrated that the subcellular distribution of the adhesion plaque protein, talin, changes dramatically in human platelets in response to platelet activation (Beckerle et al., J. Cell Biol. 109, 3333-3346, 1989). Talin is uniformly distributed throughout the cytoplasm of resting platelets. Howeve...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Nuclear lamin A and C alleles that are linked to three distinct human diseases have been expressed both in HeLa cells and in fibroblasts derived from Lmna null mice. Point mutations that cause dilated cardiomyopathy (L85R and N195K) and autosomal dominant Emery-Dreifuss muscular dystrophy (L530P) modify the assembly p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
