Abstract:
:Intercellular propagation of signals through connexin32-containing gap junctions is of major importance in physiological processes like nerve activity-dependent glucose mobilization in liver parenchymal cells and enzyme secretion from pancreatic acinar cells. In these cells, as in other organs, more than one type of connexin is expressed. We hypothesized that different permeabilities towards second messenger molecules could be one of the reasons for connexin diversity. In order to investigate this, we analyzed transmission of inositol 1,4,5-trisphosphate-mediated calcium waves in FURA-2-loaded monolayers of human HeLa cells expressing murine connexin26, -32 or -43. Gap junction-mediated cell coupling in different connexin-transfected HeLa cells was standardized by measuring the spreading of microinjected Mn(2+) that led to local quenching of FURA-2 fluorescence. Microinjection of inositol 1,4,5-trisphosphate into confluently growing HeLa connexin32 transfectants induced propagation of a Ca(2+) wave from the injected cell to neighboring cells that was at least three- to fourfold more efficient than in HeLa Cx26 cells and about 2.5-fold more efficient than in HeLa Cx43 transfectants. Our results support the notion that diffusion of inositol 1,4,5-trisphosphate through connexin32-containing gap junctions is essential for the optimal physiological response, for example by recruiting liver parenchymal cells that contain subthreshold levels of this short lived second messenger.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Niessen H,Harz H,Bedner P,Krämer K,Willecke Kkeywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
1365-72eissn
0021-9533issn
1477-9137journal_volume
113 ( Pt 8)pub_type
杂志文章abstract::Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA. Using a double-labelling procedure we have compared, at different salt concentrations, t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Cells that have been pre-exposed to thermal stress can acquire a transient resistance against the killing effect of a subsequent thermal stress. The cause for this phenomenon, called thermotolerance, seems to be an enhanced resistance of proteins against thermal denaturation and aggregation. This resistance can be exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Autophagy requires the formation of membrane vesicles, known as autophagosomes, that engulf cellular cargoes and subsequently recruit lysosomal hydrolases for the degradation of their contents. A number of autophagy-related proteins act to mediate the de novo biogenesis of autophagosomes and vesicular trafficking even...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.249227
更新日期:2020-10-30 00:00:00
abstract::In plant cells nucleolar fibrillar centres (FCs) undergo ultrastructural changes, depending on the nucleolar activity. We have found two types of FC structure in nucleoli with either high or low activity, to which we have given the conventional names of homogeneous and heterogeneous, respectively. The first type is ch...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::In many physiological contexts, intracellular reduction-oxidation (redox) conditions and the unfolded protein response (UPR) are important for the control of cell life and death decisions. UPR is triggered by the disruption of endoplasmic reticulum (ER) homeostasis, also known as ER stress. Depending on the duration a...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.153643
更新日期:2014-09-01 00:00:00
abstract::The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.206417
更新日期:2018-05-10 00:00:00
abstract::Adaptors are the membrane-binding components of clathrin-coated vesicles. The interaction of the trans-Golgi coat adaptor AP1 with membrane-associated proteins was analyzed by affinity chromatography. Proteins of 83 and 52 kDa bound specifically to the core domain of AP1 and showed no interaction with AP2 or other cla...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00302
更新日期:2003-04-01 00:00:00
abstract::TAR DNA-binding protein (TDP-43, also known as TARDBP) is the major pathological protein in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Large TDP-43 aggregates that are decorated with degradation adaptor proteins are seen in the cytoplasm of remaining neurons in ALS and FTD patients post mor...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.140087
更新日期:2014-03-15 00:00:00
abstract::Aberrant glycosylation of proteins and lipids is a common feature of many tumor cell types, and is often accompanied by alterations in membrane traffic and an anomalous localization of Golgi-resident proteins and glycans. These observations suggest that the Golgi complex is a key organelle for at least some of the fun...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.101758
更新日期:2012-08-01 00:00:00
abstract::A Mr 60,000 protein of the axonal cortical cytoplasm, which is recognized by a novel monoclonal antibody, is described. The antibody, DR1, was produced by immunizing mice with a soluble extract of bovine brain membranes that is enriched in known membrane cytoskeletal proteins. DR1 recognizes a Mr 60,000 protein in thi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Serial sections of mitotic spindles of the marine cryptophycean alga, Cryptomonas, were analysed to determine what types of microtubules they contained and which of these microtubules came close enough to each other (50 nm or less) for the commonly described crossbridging to be possible. Interpolar microtubules were r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells is a pathological process that occurs during peritoneal dialysis. EMT leads to peritoneal fibrosis, ultrafiltration failure and eventually to the discontinuation of therapy. Signaling pathways involved in mesothelial EMT are thus of great inter...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.071647
更新日期:2010-12-15 00:00:00
abstract::Galectin-3 is a member of a closely related family of beta-galactoside-binding soluble proteins found in many vertebrate epithelial and myeloid cell types. The developmentally regulated presence of galectin-3 in tissues, for example kidney, and an affinity for many cell-surface and matrix glycoproteins indicate its im...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The distribution of centromeres in the interphase nuclei of human diploid fibroblasts was analyzed using anti-centromere immunofluorescence and laser scanning confocal microscopoy. The positions of the centromeres were placed within the nuclear chromatin distribution and presented some aspects of the dynamics of nucle...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::The weak spindle integrity checkpoint in Drosophila spermatocytes has revealed a novel function of the gamma-tubulin ring complex (gammaTuRC) in maintaining spindle bipolarity throughout meiosis. Bipolar and bi-astral spindles could form in Drosophila mutants for dd4, the gene encoding the 91 kDa subunit of gammaTuRC....
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00295
更新日期:2003-03-01 00:00:00
abstract::Parasite surfaces support multiple functions required for survival within their hosts, and maintenance and functionality of the surface depends on membrane trafficking. To understand the evolutionary history of trypanosomatid trafficking, where multiple lifestyles and mechanisms of host interactions are known, we exam...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.197640
更新日期:2017-04-15 00:00:00
abstract::Nearly all intermediate filament (IF) proteins share two sequence motifs located at the N- and the C-terminal ends of their helical rod domain ('coil 1a' and 'coil 2b', respectively). To examine the structural role of the coil 2b motif, we have performed in vitro assembly studies and in vivo microinjection experiments...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::Dyneins are motor proteins that move along microtubules. They have many roles in the cell. They drive the beating of cilia and flagella, move cargos in the cytoplasm and function in the mitotic spindle. Dyneins are large and complex protein machines. Until recently, the way they move was poorly understood. In 2012, tw...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.120725
更新日期:2013-02-01 00:00:00
abstract::E-cadherin is the main cell adhesion molecule of early embryonic and adult epithelial cells. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic mesoderm formation and tumor progression. To identify genes whose expression is affected by the loss of E-cadherin, we compared...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Motile eukaryotic flagella beat through coordinated activity of dynein motor proteins; however, the mechanisms of dynein coordination and regulation are incompletely understood. The inner dynein arm (IDA) f complex (also known as the I1 complex), and the tether and tether head (T/TH) complex are thought to be key regu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.239855
更新日期:2020-01-23 00:00:00
abstract::Protein with tau-like repeats (PTL-1) is the sole Caenorhabditis elegans homolog of tau and MAP2, which are members of the mammalian family of microtubule-associated proteins (MAPs). In mammalian neurons, tau and MAP2 are segregated, with tau being mainly localised to the axon and MAP2 mainly to the dendrite. In parti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.jcs124404
更新日期:2013-05-01 00:00:00
abstract::A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Lucké Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::The protein product of the gene responsible for ocular albinism type 1, named OA1, is a pigment-cell-specific membrane glycoprotein, displaying features of G-protein-coupled receptors, yet exclusively localized to late endosomes, lysosomes and melanosomes. To dissect the signals responsible for the intracellular local...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02930
更新日期:2006-05-15 00:00:00
abstract::The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216101
更新日期:2018-07-09 00:00:00
abstract::A specific mechanism for the intracellular translocation of nonvesicle-associated proteins is proposed. This movement machinery is based on the assumption that the cytoskeleton represents an interconnected network of filamentous macromolecules, which extends over the entire cytoplasm. Diffusion along the filaments pro...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::When they are fixed with glutaraldehyde in the presence of calcium ions, the plasma membranes of Beroe giant smooth muscle fibres display micropapillae filled with an electron-dense deposit. After freeze-fracturing of fixed tissue, the micropapillae are still present, therefore their shape and size are determined duri...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Hyperthermia adversely affects cell structure and function, but also induces adaptive responses that allow cells to tolerate these stressful conditions. For example, heat-induced expression of the molecular chaperone protein HSP70 can prevent stress-induced cell death by inhibiting signaling pathways that lead to apop...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.081745
更新日期:2011-07-15 00:00:00