Abstract:
:We previously showed that the cell-cell junction protein plakoglobin (PG) not only suppresses motility of keratinocytes in contact with each other, but also, unexpectedly, of single cells. Here we show that PG deficiency results in extracellular matrix (ECM)-dependent disruption of mature focal adhesions and cortical actin organization. Plating PG⁻/⁻ cells onto ECM deposited by PG+/⁻ cells partially restored normal cell morphology and inhibited PG⁻/⁻ cell motility. In over 70 adhesion molecules whose expression we previously showed to be altered in PG⁻/⁻ cells, a substantial decrease in fibronectin (FN) in PG⁻/⁻ cells stood out. Re-introduction of PG into PG⁻/⁻ cells restored FN expression, and keratinocyte motility was reversed by plating PG⁻/⁻ cells onto FN. Somewhat surprisingly, based on previously reported roles for PG in regulating gene transcription, PG-null cells exhibited an increase, not a decrease, in FN promoter activity. Instead, PG was required for maintenance of FN mRNA stability. PG⁻/⁻ cells exhibited an increase in activated Src, one of the kinases controlled by FN, a phenotype reversed by plating PG⁻/⁻ cells on ECM deposited by PG+/⁻ keratinocytes. PG⁻/⁻ cells also exhibited Src-independent activation of the small GTPases Rac1 and RhoA. Both Src and RhoA inhibition attenuated PG⁻/⁻ keratinocyte motility. We propose a novel role for PG in regulating cell motility through distinct ECM-Src and RhoGTPase-dependent pathways, influenced in part by PG-dependent regulation of FN mRNA stability.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Todorović V,Desai BV,Patterson MJ,Amargo EV,Dubash AD,Yin T,Jones JC,Green KJdoi
10.1242/jcs.070391subject
Has Abstractpub_date
2010-10-15 00:00:00pages
3576-86issue
Pt 20eissn
0021-9533issn
1477-9137pii
jcs.070391journal_volume
123pub_type
杂志文章abstract::We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::Xenopus egg extracts, which support nuclear assembly and DNA replication, were functionally depleted of lamin LIII by inoculating them with monoclonal anti-lamin antibodies. Phase-contrast microscopy and electron-microscopy studies indicated that lamin-depleted extracts supported efficient chromatin decondensation, an...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::In some individuals with autosomal dominant isolated growth hormone deficiency, one copy of growth hormone lacks amino acids 32-71 and is severely misfolded. We transfected COS7 cells with either wild-type human growth hormone or Delta 32-71 growth hormone and investigated subcellular localization of growth hormone an...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract::In many epithelial cells, epidermal growth factor (EGF) augments the epithelial-mesenchymal transition (EMT) that occurs when cells are treated with transforming growth factor β (TGFβ). We demonstrate that this augmentation requires activation of SH2 domain-containing phosphatase-2 (SHP2; also known as PTPN11), a prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.169599
更新日期:2015-11-01 00:00:00
abstract::During spermatogenesis, the first morphological indication of spermatogonia differentiation is incomplete cytokinesis, followed by the assembly of stable intercellular cytoplasmic communications. This distinctive feature of differentiating male germ cells has been highly conserved during evolution, suggesting that reg...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00163
更新日期:2002-12-15 00:00:00
abstract::Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.105163
更新日期:2012-11-15 00:00:00
abstract::Polarity of many cell types is controlled by a protein complex consisting of Bazooka/PAR-3 (Baz), PAR-6 and atypical protein kinase C (aPKC). In Drosophila, the Baz-PAR-6-aPKC complex is required for the control of cell polarity in the follicular epithelium, in ectodermal epithelia and neuroblasts. aPKC is the main si...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.052514
更新日期:2009-10-15 00:00:00
abstract::In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::The assembly of an organism requires the interaction between different layers of cells, in many cases via an extracellular matrix. In the developing Drosophila larva, muscles attach in an integrin-dependent manner to the epidermis, via a specialized extracellular matrix called tendon matrix. Tiggrin, a tendon matrix i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Mitochondria play essential roles in cellular energy processes, including ATP production, control of reactive oxygen species (ROS) and apoptosis. While mitochondrial function is regulated by the dynamics of fusion and fission, mitochondrial homeostasis remains incompletely understood. Recent studies implicate dynamin-...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.204537
更新日期:2017-07-15 00:00:00
abstract::Assembly of the nuclear envelope (NE) in telophase is essential for higher eukaryotic cells to re-establish a functional nucleus. Time-lapse, FRAP and FRET analyses in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct ;core' region of the telophase ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.033597
更新日期:2008-08-01 00:00:00
abstract::Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.161554
更新日期:2015-04-15 00:00:00
abstract::Tie2-promoter-mediated loss of peroxisome proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of PPARγ in PMVE...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.169011
更新日期:2016-02-15 00:00:00
abstract::When expressed in pituitary AtT-20 cells, parotid proline-rich proteins enter the regulated pathway. Because the short N-terminal domain of a basic proline-rich protein is necessary for efficient export from the ER, it has not been possible to evaluate the role of this polypeptide segment as a sorting signal for regul...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Cells that are treated long-term with TNFalpha or short-term with TGFalpha together with cycloheximide (CHX) undergo apoptosis. Cell shrinkage and detachment during apoptosis is dependent on actomyosin contractility. Myosin II heavy chain (MHCII) isoforms have shared and distinct functions. Here, we investigated wheth...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.022640
更新日期:2008-05-15 00:00:00
abstract::The Down syndrome critical region (DSCR) on Chromosome 21 contains many genes whose duplication may lead to the major phenotypic features of Down syndrome and especially the associated mental retardation. However, the functions of DSCR genes are mostly unknown and their possible involvement in key brain developmental ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.000703
更新日期:2007-06-01 00:00:00
abstract::Our previous studies of PAWS1 (protein associated with SMAD1; also known as FAM83G) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1-knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.202390
更新日期:2018-01-10 00:00:00
abstract::Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.173195
更新日期:2016-01-15 00:00:00
abstract::Cartilage oligomeric matrix protein (COMP) is an abundant component in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.180216
更新日期:2016-02-15 00:00:00
abstract::Cyclins control the activities of cyclin-dependent protein kinases (cdks) and hence play a key role in cell cycle regulation. While B-type cyclins associate with p34cdc2 to trigger entry into mitosis, progression through S phase requires cyclin A, presumably in association with p33cdk2. Vertebrate A- and B-type cyclin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::The extracellular environment and tissue architecture contribute to proper cell function and growth control. Cells growing in monolayers on standard polystyrene tissue culture plates differ in their shape, growth rate and response to external stimuli, compared with cells growing in vivo. Here, we showed that the EGFR ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.054551
更新日期:2009-12-01 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulatio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.174235
更新日期:2016-01-01 00:00:00
abstract::The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that ha...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::The expression level and functional activity of estrogen receptor alpha is an important determinant of breast physiology and breast cancer treatment. However, it has been difficult to identify the signals that regulate estrogen receptor because cultured mammary epithelial cells generally do not respond to estrogenic s...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00523
更新日期:2003-07-15 00:00:00
abstract::Annexin 1 is a Ca(2+)-regulated membrane binding protein and a major substrate of the epidermal growth factor receptor kinase. Because of its properties and intracellular distribution, the protein has been implicated in endocytic trafficking of the receptor, in particular in receptor sorting occurring in multivesicula...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state and a GDP-bound inactive state. This cycle is regulated by guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs and GAPs to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216630
更新日期:2018-09-03 00:00:00
abstract::Elementary Ca(2+) signals, such as "Ca(2+) puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca(2+) signalling. We characterized Ca(2+) puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor is...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::In order to study the dynamic behavior of intermediate filament networks in living cells, we have prepared constructs fusing green fluorescent protein to intermediate filament proteins. Vimentin fused to green fluorescent protein labeled the endogenous intermediate filament network. We generated stable SW13 and NIH3T3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Mouse oocytes are induced by cytochalasin B to undergo 'pseudo-cleavage' in vitro into 2 equally sized and separable compartments. This response to the drug is dependent upon the meiotic state of the oocytes, as well as upon the presence of an intact zona pellucida. The resulting 2 cellular compartments can be complet...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membran...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00