Kinase-activity-independent functions of atypical protein kinase C in Drosophila.

abstract：:The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate c...

journal_title：Journal of cell science

authors： Harrison RE,Sikorski BA,Jongstra J

更新日期：2004-04-15 00:00:00

abstract：:Maintenance of epithelial polarity depends on the correct localization and levels of polarity determinants. The evolutionarily conserved transmembrane protein Crumbs is crucial for the size and identity of the apical membrane, yet little is known about the molecular mechanisms controlling the amount of Crumbs at the s...

journal_title：Journal of cell science

authors： Lin YH,Currinn H,Pocha SM,Rothnie A,Wassmer T,Knust E

更新日期：2015-12-15 00:00:00

abstract：:Cannabinoid receptors (CBRs) belong to the G protein-coupled receptor superfamily, and activation of CBRs in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes in acinar cell physiology and in intracellular signalling remains unclear. Here, we...

journal_title：Journal of cell science

authors： Kopach O,Vats J,Netsyk O,Voitenko N,Irving A,Fedirko N

更新日期：2012-04-15 00:00:00

abstract：:Aberrant glycosylation of proteins and lipids is a common feature of many tumor cell types, and is often accompanied by alterations in membrane traffic and an anomalous localization of Golgi-resident proteins and glycans. These observations suggest that the Golgi complex is a key organelle for at least some of the fun...

journal_title：Journal of cell science

authors： Babiá T,Ayala I,Valderrama F,Mato E,Bosch M,Santarén JF,Renau-Piqueras J,Kok JW,Thomson TM,Egea G

更新日期：1999-02-01 00:00:00

abstract：:Promyelocytic leukemia nuclear bodies (PML-NBs) are mobile subnuclear organelles formed by PML and Sp100 protein. They have been reported to have a role in transcription, DNA replication and repair, telomere lengthening, cell cycle control and tumor suppression. We have conducted high-resolution 4Pi fluorescence laser...

journal_title：Journal of cell science

authors： Lang M,Jegou T,Chung I,Richter K,Münch S,Udvarhelyi A,Cremer C,Hemmerich P,Engelhardt J,Hell SW,Rippe K

更新日期：2010-02-01 00:00:00

abstract：:Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found ...

journal_title：Journal of cell science

authors： Rodríguez-Sánchez L,Rodríguez-López M,García Z,Tenorio-Gómez M,Schvartzman JB,Krimer DB,Hernández P

更新日期：2011-01-01 00:00:00

abstract：:The interplay between pathogen-encoded virulence factors and host cell signaling networks is critical for both the establishment and clearance of microbial infections. Yersinia uptake into host cells serves as an in vitro model for exploring how host cells respond to Yersinia adherence. In this study, we provide insig...

journal_title：Journal of cell science

authors： Bruce-Staskal PJ,Weidow CL,Gibson JJ,Bouton AH

更新日期：2002-07-01 00:00:00

abstract：:The specification of organs, tissues and cell types results from cell fate restrictions enacted by nuclear transcription factors under the control of conserved signaling pathways. The progenitor epithelium of the Drosophila compound eye, the eye imaginal disc, is a premier model for the study of such processes. Early ...

journal_title：Journal of cell science

authors： Neal SJ,Zhou Q,Pignoni F

更新日期：2020-05-26 00:00:00

abstract：:Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...

journal_title：Journal of cell science

authors： Krag C,Malmberg EK,Salcini AE

更新日期：2010-12-15 00:00:00

abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

journal_title：Journal of cell science

authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

更新日期：2014-06-01 00:00:00

abstract：:Rac1 has a crucial role in epidermal growth factor (EGF)-induced membrane ruffling, lamellipodial protrusion, and cell migration. Several guanine nucleotide exchange factors (GEFs) including Sos1, Sos2, Tiam1 and Vav2 have been shown to transduce the growth signal from the EGF receptor to Rac1. To clarify the role of ...

journal_title：Journal of cell science

authors： Itoh RE,Kiyokawa E,Aoki K,Nishioka T,Akiyama T,Matsuda M

更新日期：2008-08-15 00:00:00

abstract：:The plasma membrane Ca2+-ATPase-2 (PMCA2) is expressed in stereocilia of hair cells of the inner ear, whereas PMCA1 is expressed in the basolateral plasma membrane of hair cells. Both extrude excess Ca2+ from the cytosol. They are predicted to contain ten membrane-spanning segments, two large cytoplasmic loops as well...

journal_title：Journal of cell science

authors： Grati M,Aggarwal N,Strehler EE,Wenthold RJ

更新日期：2006-07-15 00:00:00

abstract：:Keratins 8 and 18 (collectively referred to as K8/K18) are the major components of intermediate filaments of simple epithelial cells. Recent studies have revealed the function of K8/K18 in apoptosis modulation. Here, we show that eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation...

journal_title：Journal of cell science

authors： Lin YM,Chen YR,Lin JR,Wang WJ,Inoko A,Inagaki M,Wu YC,Chen RH

更新日期：2008-07-15 00:00:00

abstract：:Nodal signaling induces the formation of the endoderm and mesoderm during gastrulation. Nodal expression persists until the definitive endoderm progenitor has completely formed, and disappears thereafter. A tightly regulated Nodal expression system is essential for the differentiation of embryonic stem (ES) cells into...

journal_title：Journal of cell science

authors： Takenaga M,Fukumoto M,Hori Y

更新日期：2007-06-15 00:00:00

abstract：:In the developing Drosophila eye, the centrioles of the differentiating retinal cells are not surrounded by the microtubule-nucleating γ-tubulin, suggesting that they are unable to organize functional microtubule-organizing centers. Consistent with this idea, Cnn and Spd-2, which are involved in γ-tubulin recruitment,...

journal_title：Journal of cell science

authors： Riparbelli MG,Persico V,Gottardo M,Callaini G

更新日期：2018-02-22 00:00:00

abstract：:The degradation of misfolded proteins is essential for cellular homeostasis. Misfolded proteins are normally degraded by the ubiquitin-proteasome system (UPS), and selective autophagy serves as a backup mechanism when the UPS is overloaded. Selective autophagy mediates the degradation of harmful material by its seques...

journal_title：Journal of cell science

authors： Danieli A,Martens S

更新日期：2018-10-04 00:00:00

abstract：:Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight...

journal_title：Journal of cell science

authors： Brown RC,Mark KS,Egleton RD,Huber JD,Burroughs AR,Davis TP

更新日期：2003-02-15 00:00:00

abstract：:During the prometaphase stage of mitosis, the cell builds a bipolar spindle of microtubules that mechanically segregates sister chromatids between two daughter cells in anaphase. The spindle assembly checkpoint (SAC) is a quality control mechanism that monitors proper attachment of microtubules to chromosome kinetocho...

journal_title：Journal of cell science

authors： Courtheoux T,Diallo A,Damodaran AP,Reboutier D,Watrin E,Prigent C

更新日期：2018-04-12 00:00:00

abstract：:Endonuclease G is a mitochondrial protein implicated in DNA fragmentation during apoptosis in cell types ranging from fungi to mammals. Features of programmed cell death have been reported in a number of single-celled organisms, including the human trypanosomatid parasites Leishmania and Trypanosoma. However, the prot...

journal_title：Journal of cell science

authors： Gannavaram S,Vedvyas C,Debrabant A

更新日期：2008-01-01 00:00:00

abstract：:Cells synthesize ceramides in the endoplasmic reticulum (ER) as precursors for sphingolipids to form an impermeable plasma membrane. As ceramides are engaged in apoptotic pathways, cells would need to monitor their levels closely to avoid killing themselves during sphingolipid biosynthesis. How this is accomplished re...

journal_title：Journal of cell science

authors： Tafesse FG,Vacaru AM,Bosma EF,Hermansson M,Jain A,Hilderink A,Somerharju P,Holthuis JC

更新日期：2014-01-15 00:00:00

abstract：:Here we present experimental evidence that phagocytic cells use modulation of specific components of their glycocalyx to regulate their binding capacity. Particles coated with antibodies specific for the CD32 medium affinity IgG receptor were driven along human monocytic THP-1 cells (expressing CD32) in a flow chamber...

journal_title：Journal of cell science

authors： Sabri S,Soler M,Foa C,Pierres A,Benoliel A,Bongrand P

更新日期：2000-05-01 00:00:00

abstract：:The chromosomes of the active previtellogenic oocytes of the dragonfly Cordulia aenea L. extrude into the cytoplasm a substance, which is subsequently found there in the form of granulofibrillar masses. These nuclear extrusions evolve ultrastructurally into two components, nematosomes and dense masses. The nematosomes...

journal_title：Journal of cell science

authors： Halkka L,Halkka O

更新日期：1975-10-01 00:00:00

abstract：:The transmembrane inner nuclear membrane (INM) protein Samp1 is required for anchoring centrosomes near the nuclei. Using high-resolution fluorescence microscopy we show that Samp1 is distributed in a distinct and characteristic pattern in the nuclear envelope (NE), where it partially colocalizes with the LINC complex...

journal_title：Journal of cell science

authors： Gudise S,Figueroa RA,Lindberg R,Larsson V,Hallberg E

更新日期：2011-06-15 00:00:00

abstract：:We previously demonstrated that the aspartate protease cathepsin D is activated by ceramide derived from acid sphingomyelinase. Increased expression of cathepsin D in the skin has been reported in wound healing, psoriasis and skin tumors. We explored specific functions of cathepsin D during epidermal differentiation. ...

journal_title：Journal of cell science

authors： Egberts F,Heinrich M,Jensen JM,Winoto-Morbach S,Pfeiffer S,Wickel M,Schunck M,Steude J,Saftig P,Proksch E,Schütze S

更新日期：2004-05-01 00:00:00

abstract：:Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...

journal_title：Journal of cell science

authors： Olson ML,Sandison ME,Chalmers S,McCarron JG

更新日期：2012-11-15 00:00:00

abstract：:Genomes exist in vivo as complex physical structures, and their functional output (i.e. the gene expression profile of a cell) is related to their spatial organization inside the nucleus as well as to local chromatin status. Chromatin modifications and chromosome conformation are distinct in different tissues and cell...

journal_title：Journal of cell science

authors： Naumova N,Dekker J

更新日期：2010-06-15 00:00:00

abstract：:We have investigated the role in macrophage lysosomal enzyme release of arachidonate metabolites, extracellular divalent cations and cyclic AMP (cAMP) which modulate secretion in other cell types. Lysosomal enzyme secretion induced by zymosan was accompanied by release of malondialdehyde (MDA), which is derived from a...

journal_title：Journal of cell science

authors： McMillan RM,Macintyre DE,Beesley JE,Gordon JL

更新日期：1980-08-01 00:00:00

abstract：:The cell nucleus is highly organized. Many nuclear functions are localized in discrete domains, suggesting that compartmentalization is an important aspect of the regulation and coordination of nuclear functions. We investigated the subnuclear distribution of the glucocorticoid receptor, a hormone-dependent transcript...

journal_title：Journal of cell science

authors： van Steensel B,Brink M,van der Meulen K,van Binnendijk EP,Wansink DG,de Jong L,de Kloet ER,van Driel R

更新日期：1995-09-01 00:00:00

abstract：:Prox1 is a master regulator for the development of lymphatic vasculature and the induction of lymphangiogenesis. In this study, we identified Prox1 as a new target for small ubiquitin-like modifier 1 (SUMO-1). Lysine 556 (K556) was found to be the major sumoylation site for Prox1 in vitro and in vivo. Mutation of this...

journal_title：Journal of cell science

authors： Pan MR,Chang TM,Chang HC,Su JL,Wang HW,Hung WC

更新日期：2009-09-15 00:00:00

abstract：:Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...

journal_title：Journal of cell science

authors： McCaig CD

更新日期：1990-04-01 00:00:00