eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions.

abstract：:Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote...

journal_title：Journal of cell science

authors： Chi RJ,Torres OT,Segarra VA,Lansley T,Chang JS,Newpher TM,Lemmon SK

更新日期：2012-10-15 00:00:00

abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

journal_title：Journal of cell science

authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

更新日期：2014-06-01 00:00:00

abstract：:CK2 is a serine/threonine kinase with many substrates, largely unknown modes of regulation and essential roles in mitotic progression. CK2α, a catalytic subunit of CK2, is phosphorylated in mitosis, and here we examine the effect of phosphorylation on CK2α localization. Using phosphospecific antibodies, we show that C...

journal_title：Journal of cell science

authors： St-Denis NA,Bailey ML,Parker EL,Vilk G,Litchfield DW

更新日期：2011-07-15 00:00:00

abstract：:In adipocytes and muscle cells, the GLUT4 glucose transporter isoform is present in intracellular vesicles which continuously recycle between an intracytoplasmic location and the plasma membrane. It is not clear whether the GLUT4-vesicles represent a specific kind of vesicle or resemble typical secretory granules or s...

journal_title：Journal of cell science

authors： Thorens B,Roth J

更新日期：1996-06-01 00:00:00

abstract：:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and hande...

journal_title：Journal of cell science

authors： Yonemura Y,Li X,Müller K,Krämer A,Atigbire P,Mentrup T,Feuerhake T,Kroll T,Shomron O,Nohl R,Arndt HD,Hoischen C,Hemmerich P,Hirschberg K,Kaether C

更新日期：2016-10-15 00:00:00

abstract：:This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous 'balloon' with two boundary structures. One of these - the collar -...

journal_title：Journal of cell science

authors： Lacomble S,Vaughan S,Gadelha C,Morphew MK,Shaw MK,McIntosh JR,Gull K

更新日期：2009-04-15 00:00:00

abstract：:Asymmetric cell division can produce daughter cells with different developmental fates and is often accompanied by a difference in cell size. A number of recent genetic and in vivo imaging studies in Drosophila and Caenorhabditis elegans have begun to elucidate the mechanisms underlying the rearrangements of the cytos...

journal_title：Journal of cell science

authors： Kaltschmidt JA,Brand AH

更新日期：2002-06-01 00:00:00

abstract：:Arf GTPases regulate both the morphological and protein sorting events that are essential for membrane trafficking. Guanine nucleotide exchange factors (GEFs) specific for Arf proteins determine when and where Arf GTPases will be activated in cells. The yeast Gea2p Arf GEF is a member of an evolutionarily conserved fa...

journal_title：Journal of cell science

authors： Chantalat S,Park SK,Hua Z,Liu K,Gobin R,Peyroche A,Rambourg A,Graham TR,Jackson CL

更新日期：2004-02-15 00:00:00

abstract：:Endothelial barrier dysfunction leads to edema and vascular leak, causing high morbidity and mortality. Previously, Abl kinase inhibition has been shown to protect against vascular leak. Using the distinct inhibitory profiles of clinically available Abl kinase inhibitors, we aimed to provide a mechanistic basis for no...

journal_title：Journal of cell science

authors： Botros L,Pronk MCA,Juschten J,Liddle J,Morsing SKH,van Buul JD,Bates RH,Tuinman PR,van Bezu JSM,Huveneers S,Bogaard HJ,van Hinsbergh VWM,Hordijk PL,Aman J

更新日期：2020-05-14 00:00:00

abstract：:MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 b...

journal_title：Journal of cell science

authors： Scales TM,Lin S,Kraus M,Goold RG,Gordon-Weeks PR

更新日期：2009-07-15 00:00:00

abstract：:Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3'-OH position of the inositol ring of inositol phospholipids, producing three lipid products: PtdIns(3)P, PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3). These lipids bind to the pleckstrin homology (PH) domains of proteins and control the activity and subcellular localisat...

journal_title：Journal of cell science

authors： Cantrell DA

更新日期：2001-04-01 00:00:00

abstract：:Barrier junctions prevent pathogen invasion and restrict paracellular leakage across epithelial sheets. To understand how one barrier junction, the septate junction (SJ), is regulated in vivo, we used fluorescence recovery after photobleaching (FRAP) to examine SJ protein dynamics in Drosophila. Most SJ-associated pro...

journal_title：Journal of cell science

authors： Oshima K,Fehon RG

更新日期：2011-08-15 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) has been shown to induce skeletal muscle hypertrophy, to prevent the loss of muscle mass with ageing and to improve the muscle phenotype of dystrophic mice. We previously developed a model of IGF-1-induced hypertrophy of human myotubes, in which hypertrophy was not only characteriz...

journal_title：Journal of cell science

authors： Jacquemin V,Butler-Browne GS,Furling D,Mouly V

更新日期：2007-02-15 00:00:00

abstract：:By differential screening of a Xenopus laevis egg cDNA library, we have isolated a 2,111 bp cDNA which corresponds to a maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes a 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members of a new protein...

journal_title：Journal of cell science

authors： Roghi C,Giet R,Uzbekov R,Morin N,Chartrain I,Le Guellec R,Couturier A,Dorée M,Philippe M,Prigent C

更新日期：1998-03-01 00:00:00

abstract：:Alix is a cytosolic protein in mammalian cells that was originally identified on the basis of its association with pro-apoptotic signaling. More recent evidence has established that Alix has a hand in regulating other cellular mechanisms, including endocytic membrane trafficking and cell adhesion. Although Alix appear...

journal_title：Journal of cell science

authors： Odorizzi G

更新日期：2006-08-01 00:00:00

abstract：:During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...

journal_title：Journal of cell science

authors： Mitra J,Schultz RM

更新日期：1996-09-01 00:00:00

abstract：:Shape changes have been determined in human blood lymphocytes stimulated with OAG, diC8, PMA, colchicine or the hexapeptide fNLPNTL in short-term assays (30 min). Distinct types of shape-change responses were observed. Colchicine was active in generating a relatively small proportion of polarized lymphocytes (front-ta...

journal_title：Journal of cell science

authors： Keller HU,Niggli V,Zimmermann A

更新日期：1989-07-01 00:00:00

abstract：:Mitochondria are dynamic organelles that undergo regulated fission and fusion events that are essential to maintain metabolic stability. We previously demonstrated that the mitochondrial fission GTPase DRP1 is a substrate for SUMOylation. To further understand how SUMOylation impacts mitochondrial function, we searche...

journal_title：Journal of cell science

authors： Zunino R,Schauss A,Rippstein P,Andrade-Navarro M,McBride HM

更新日期：2007-04-01 00:00:00

abstract：:Polarity of many cell types is controlled by a protein complex consisting of Bazooka/PAR-3 (Baz), PAR-6 and atypical protein kinase C (aPKC). In Drosophila, the Baz-PAR-6-aPKC complex is required for the control of cell polarity in the follicular epithelium, in ectodermal epithelia and neuroblasts. aPKC is the main si...

journal_title：Journal of cell science

authors： Kim S,Gailite I,Moussian B,Luschnig S,Goette M,Fricke K,Honemann-Capito M,Grubmüller H,Wodarz A

更新日期：2009-10-15 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway, Spitz is cleaved within its...

journal_title：Journal of cell science

authors： Steinhauer J,Liu HH,Miller E,Treisman JE

更新日期：2013-10-01 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:When HeLa cells are lysed in solutions containing a non-ionic detergent and 2 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is supercoiled and so intact. Using ...

journal_title：Journal of cell science

authors： Levin JM,Cook PR

更新日期：1981-08-01 00:00:00

abstract：:The mechanistic target of rapamycin (mTOR) signaling pathway coordinates environmental and intracellular cues to control eukaryotic cell growth. As a pivot point between anabolic and catabolic processes, mTOR complex 1 (mTORC1) signaling has established roles in regulating metabolism, translation and autophagy. Hypera...

journal_title：Journal of cell science

authors： Condon KJ,Sabatini DM

更新日期：2019-11-13 00:00:00

abstract：:We have isolated suppressors of the deletion allele of delta-tubulin, uni3-1, in the biflagellate green alga Chlamydomonas reinhardtii. The deletion of delta-tubulin produces cells that assemble zero, one or two flagella and have basal bodies composed primarily of doublet rather than triplet microtubules. Flagellar nu...

journal_title：Journal of cell science

authors： Fromherz S,Giddings TH Jr,Gomez-Ospina N,Dutcher SK

更新日期：2004-01-15 00:00:00

abstract：:Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...

journal_title：Journal of cell science

authors： Brasseur A,Bayat S,Chua XL,Zhang Y,Zhou Q,Low BC,He CY

更新日期：2014-11-15 00:00:00

abstract：:Sea urchin coelomocytes undergo an inducible structural transformation from petalloid to filopodial form during the 'clotting' response in sea urchins. Using a petalloid coelomocyte model, stimulated coelomocytes exhibited bidirectional particle/vesicle motility with a broad distribution of velocities, ranging from 0....

journal_title：Journal of cell science

authors： D'Andrea L,Danon MA,Sgourdas GP,Bonder EM

更新日期：1994-08-01 00:00:00

abstract：:It has been suggested by many studies that Ca2+ signaling plays an important role in regulating key steps in cell division. In order to study the down stream components of calcium signaling, we have fused the gene of calmodulin (CaM) with that of green fluorescent protein (GFP) and expressed it in HeLa cells. The GFP-...

journal_title：Journal of cell science

authors： Li CJ,Heim R,Lu P,Pu Y,Tsien RY,Chang DC

更新日期：1999-05-01 00:00:00

abstract：:Transcriptional activation of the major histocompatibility complex (MHC) by IFNgamma is a key step in cell-mediated immunity. At an early stage of IFNgamma induction, chromatin carrying the entire MHC locus loops out from the chromosome 6 territory. We show here that JAK/STAT signalling triggers this higher-order chro...

journal_title：Journal of cell science

authors： Christova R,Jones T,Wu PJ,Bolzer A,Costa-Pereira AP,Watling D,Kerr IM,Sheer D

更新日期：2007-09-15 00:00:00

abstract：:Kindlin-2 is a FERM and PH domain-containing integrin-binding protein that is emerging as an important regulator of integrin activation. How kindlin-2 functions in integrin activation, however, is not known. We report here that kindlin-2 interacts with multiple phosphoinositides, preferentially with phosphatidylinosit...

journal_title：Journal of cell science

authors： Qu H,Tu Y,Shi X,Larjava H,Saleem MA,Shattil SJ,Fukuda K,Qin J,Kretzler M,Wu C

更新日期：2011-03-15 00:00:00

abstract：:Mitosis involves considerable membrane remodelling and vesicular trafficking to generate two independent cells. Consequently, endocytosis and endocytic proteins are required for efficient mitotic progression and completion. Several endocytic proteins also participate in mitosis in an endocytosis-independent manner. He...

journal_title：Journal of cell science

authors： Ma MP,Chircop M

更新日期：2012-09-15 00:00:00