Abstract:
:Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight junction protein expression, and studied potential mechanisms of action. We found that C6-glioma-conditioned media, but not rat astrocyte-conditioned media, protected against an increase in permeability induced by exposure to 1% oxygen for 24 hours. This hypoxic stress caused an increase in the expression of tight junction proteins claudin-1 and actin, particularly in cells treated with C6-conditioned media. We found that C6-conditioned media has a significantly higher level of both basic fibroblast growth factor and vascular endothelial growth factor. Treatment with C6-conditioned media for 1 or 3 days protects against hypoxia-induced permeability increases, and this protective effect may be mediated by signal transduction pathways terminating at the transcription factor NFkappaB.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Brown RC,Mark KS,Egleton RD,Huber JD,Burroughs AR,Davis TPdoi
10.1242/jcs.00264keywords:
subject
Has Abstractpub_date
2003-02-15 00:00:00pages
693-700issue
Pt 4eissn
0021-9533issn
1477-9137journal_volume
116pub_type
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