Adhesion of neutrophil leucocytes under conditions of flow.

Abstract:

:By passing a suspension of polymorphonuclear leucocytes through a parallel-plate chamber their adhesiveness can be assessed by scoring the number of cells trapped on the lower plate, and the fluid shear stress can be defined for a given flow rate. Since the adhesiveness of the cell at the instant of collision must exceed the distractive shear if the cell is to stop, the kinetics of cell accumulation provide a measure of the adhesiveness of the leucocytes and the adhesive interaction can be quantified. This measure of adhesion does not suffer from the complication that the force required to remove the cells from the surface will be greater if the cells have the opportunity to spread before the distractive force is applied. The assay is described in detail and the results of modifying the surface of the flow chamber and altering the composition of the suspension medium are used to illustrate the method. Plasma proteins generally seemed to reduce the adhesiveness of neutrophil leucocytes, whether they were present as a coat of adsorbed protein or in the suspension medium during perfusion. Neutrophil leucocytes, unless suspended in relatively high concentrations of plasma, were considerably more adhesive than other cells that have been tested in this assay system.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Forrester JV,Lackie JM

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

93-110

eissn

0021-9533

issn

1477-9137

journal_volume

70

pub_type

杂志文章
  • The ubiquitin-proteasome pathway regulates survivin degradation in a cell cycle-dependent manner.

    abstract::Survivin, a human inhibitor of apoptosis protein (IAP), plays an important role in both cell cycle regulation and inhibition of apoptosis. Survivin is expressed in cells during the G(2)/M phase of the cell cycle, followed by rapid decline of both mRNA and protein levels at the G(1) phase. It has been suggested that ce...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Zhao J,Tenev T,Martins LM,Downward J,Lemoine NR

    更新日期:2000-12-01 00:00:00

  • The 12 kDa FK506-binding protein, FKBP12, modulates the Ca(2+)-flux properties of the type-3 ryanodine receptor.

    abstract::We have characterised the functional regulation of the type-3 ryanodine receptor by the 12 kDa FK506-binding protein. Wild-type type-3 ryanodine receptor and mutant type-3 ryanodine receptor in which the critical valine at position 2322 in the central 12 kDa FK506-binding protein binding site was substituted by aspart...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00948

    authors: Van Acker K,Bultynck G,Rossi D,Sorrentino V,Boens N,Missiaen L,De Smedt H,Parys JB,Callewaert G

    更新日期:2004-03-01 00:00:00

  • Fractionation of human liver mitochondria: enzymic and morphological characterization of the inner and outer membranes as compared to rat liver mitochondria.

    abstract::The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membran...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Benga G,Hodarnau A,Tilinca R,Porutiu D,Dancea S,Pop V,Wrigglesworth J

    更新日期:1979-02-01 00:00:00

  • Dual role for microtubules in regulating cortical contractility during cytokinesis.

    abstract::Microtubules stimulate contractile-ring formation in the equatorial cortex and simultaneously suppress contractility in the polar cortex; how they accomplish these differing activities is incompletely understood. We measured the behavior of GFP-actin in mammalian cells treated with nocodazole under conditions that eit...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.027052

    authors: Murthy K,Wadsworth P

    更新日期:2008-07-15 00:00:00

  • TNFα induced noncanonical NF-κB activation is attenuated by RIP1 through stabilization of TRAF2.

    abstract::The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.075770

    authors: Kim JY,Morgan M,Kim DG,Lee JY,Bai L,Lin Y,Liu ZG,Kim YS

    更新日期:2011-02-15 00:00:00

  • INPP5E interacts with AURKA, linking phosphoinositide signaling to primary cilium stability.

    abstract::Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause the ciliopathies known as Joubert and MORM syndromes; however, the role of INPP5E in ciliary biology is not well understood. Here, we describe an interaction between INPP5E and AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly,...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.161323

    authors: Plotnikova OV,Seo S,Cottle DL,Conduit S,Hakim S,Dyson JM,Mitchell CA,Smyth IM

    更新日期:2015-01-15 00:00:00

  • Effect of targeted expression of clusterin in photoreceptor cells on retinal development and differentiation.

    abstract::Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Jomary C,Chatelain G,Michel D,Weston A,Neal MJ,Jones SE

    更新日期:1999-05-01 00:00:00

  • Calmodulin antagonist affects peroxisomal functionality by disrupting both peroxisomal Ca2+ and protein import.

    abstract::Ca2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric oxide (NO) generation is dependent on Ca2+ and calmodulin (CaM). Here, we used Arabi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.201467

    authors: Corpas FJ,Barroso JB

    更新日期:2018-01-29 00:00:00

  • Rab32 is essential for maintaining functional acidocalcisomes, and for growth and infectivity of Trypanosoma cruzi.

    abstract::The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmo...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.169466

    authors: Niyogi S,Jimenez V,Girard-Dias W,de Souza W,Miranda K,Docampo R

    更新日期:2015-06-15 00:00:00

  • An investigation of the molecular components of desmosomes in epithelial cells of five vertebrates.

    abstract::We have shown previously, by fluorescent antibody staining, that desmosomal antigens are widely distributed in the tissues of vertebrate animals. Furthermore, we have demonstrated mutual desmosome formation between cells derived from man, cow, dog, chicken and frog. In this paper we have studied the components of desm...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Suhrbier A,Garrod D

    更新日期:1986-03-01 00:00:00

  • Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase.

    abstract::Present in organisms ranging from yeast to man, homologues of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme CDC34 have been shown to play important roles in the regulation of cell cycle progression and checkpoint function. Here we analyze the expression and intracellular localization of endogenous CDC34 du...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Reymond F,Wirbelauer C,Krek W

    更新日期:2000-05-01 00:00:00

  • Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides.

    abstract::The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Rabouille C,Hui N,Hunte F,Kieckbusch R,Berger EG,Warren G,Nilsson T

    更新日期:1995-04-01 00:00:00

  • Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion.

    abstract::Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illu...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.213462

    authors: Serrano A,Illgen J,Brandt U,Thieme N,Letz A,Lichius A,Read ND,Fleißner A

    更新日期:2018-05-04 00:00:00

  • OPA1 processing in cell death and disease - the long and short of it.

    abstract::The regulation of mitochondrial dynamics by the GTPase OPA1, which is located at the inner mitochondrial membrane, is crucial for adapting mitochondrial function and preserving cellular health. OPA1 governs the delicate balance between fusion and fission in the dynamic mitochondrial network. A disturbance of this bala...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.159186

    authors: MacVicar T,Langer T

    更新日期:2016-06-15 00:00:00

  • Src mediates the mechanical activation of myogenesis by activating TNFα-converting enzyme.

    abstract::Mechanical stimulation affects many biological aspects in living cells through mechanotransduction. In myogenic precursor cells (MPCs), mechanical stimulation activates p38 mitogen-activated protein kinase (MAPK), a key regulator of myogenesis, via activating TNFα-converting enzyme (TACE, also known as ADAM17), to rel...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.125328

    authors: Niu A,Wen Y,Liu H,Zhan M,Jin B,Li YP

    更新日期:2013-10-01 00:00:00

  • A new mitochondrial pool of cyclin E, regulated by Drp1, is linked to cell-density-dependent cell proliferation.

    abstract::The regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE c...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.172429

    authors: Parker DJ,Iyer A,Shah S,Moran A,Hjelmeland AB,Basu MK,Liu R,Mitra K

    更新日期:2015-11-15 00:00:00

  • Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixing.

    abstract::Intracellular membrane fusion occurs with exquisite coordination and specificity. Each fusion event requires three basic components: Rab-GTPases organize the fusion site; SNARE proteins act during fusion; and N-ethylmaleimide-sensitive factor (NSF) plus its cofactor alpha-SNAP are required for recycling or activation ...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.02561

    authors: Ungermann C,Langosch D

    更新日期:2005-09-01 00:00:00

  • Effect of isolated plasma membranes on cell cohesion in the cellular slime mould.

    abstract::The effects of isolated plasma membranes on cohesion of Dictyostelium discoideum Ax-2 cells have been studied. Membranes isolated from cells in the log phase of growth gave complete inhibition of log-phase cell cohesion. This effect was specific for the cohesion log-phase cells mediated by contact sites B, since the c...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Jaffé AR,Garrod DR

    更新日期:1979-12-01 00:00:00

  • Dual functions of transglutaminase in novel cell adhesion.

    abstract::Transglutaminases (TGases) are enzymes which catalyze cross-link formation between glutamine residues and lysine residues in substrate proteins. In the present study, we report for the first time that a representative enzyme, blood coagulation factor XIIIa (FXIIIa), is capable of mediating adhesion of various cells. W...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Ueki S,Takagi J,Saito Y

    更新日期:1996-11-01 00:00:00

  • Differential expression of sucrase-isomaltase in clones isolated from early and late passages of the cell line Caco-2: evidence for glucose-dependent negative regulation.

    abstract::The expression of the brush border-associated hydrolase sucrase-isomaltase was shown to increase from early to late passages of Caco-2 cells, concomitant with a decrease in the rates of glucose consumption. Twenty-six clones were isolated from early (P29) and late (P198) passages of the cell line. These clones show co...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Chantret I,Rodolosse A,Barbat A,Dussaulx E,Brot-Laroche E,Zweibaum A,Rousset M

    更新日期:1994-01-01 00:00:00

  • A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells.

    abstract::One of the hallmarks of cancer is the ability of tumor cells to invade surrounding tissues and metastasize. During metastasis, cancer cells degrade the extracellular matrix, which acts as a physical barrier, by developing specialized actin-rich membrane protrusion structures called invadopodia. The formation of invado...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.195552

    authors: Goicoechea SM,Zinn A,Awadia SS,Snyder K,Garcia-Mata R

    更新日期:2017-03-15 00:00:00

  • Interphase-specific association of intrinsic centromere protein CENP-C with HDaxx, a death domain-binding protein implicated in Fas-mediated cell death.

    abstract::CENP-C, one of the few known intrinsic proteins of the human centromere, is thought to play structural as well as regulatory roles crucial to proper chromosome segregation and mitotic progression. To further define the functions of CENP-C throughout the cell cycle we have used the yeast interaction trap to identify pr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Pluta AF,Earnshaw WC,Goldberg IG

    更新日期:1998-07-30 00:00:00

  • Bosutinib prevents vascular leakage by reducing focal adhesion turnover and reinforcing junctional integrity.

    abstract::Endothelial barrier dysfunction leads to edema and vascular leak, causing high morbidity and mortality. Previously, Abl kinase inhibition has been shown to protect against vascular leak. Using the distinct inhibitory profiles of clinically available Abl kinase inhibitors, we aimed to provide a mechanistic basis for no...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.240077

    authors: Botros L,Pronk MCA,Juschten J,Liddle J,Morsing SKH,van Buul JD,Bates RH,Tuinman PR,van Bezu JSM,Huveneers S,Bogaard HJ,van Hinsbergh VWM,Hordijk PL,Aman J

    更新日期:2020-05-14 00:00:00

  • Beta2-chimaerin provides a diacylglycerol-dependent mechanism for regulation of adhesion and chemotaxis of T cells.

    abstract::The small GTPase Rac contributes to regulation of cytoskeletal rearrangement during chemokine-induced lymphocyte adhesion and migration in a multi-step process that is very precisely coordinated. Chimaerins are Rac1-specific GTPase-activating proteins of unknown biological function, which have a canonical diacylglycer...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02722

    authors: Siliceo M,García-Bernal D,Carrasco S,Díaz-Flores E,Coluccio Leskow F,Teixidó J,Kazanietz MG,Mérida I

    更新日期:2006-01-01 00:00:00

  • Microdomains of muscarinic acetylcholine and Ins(1,4,5)P₃ receptors create 'Ins(1,4,5)P₃ junctions' and sites of Ca²+ wave initiation in smooth muscle.

    abstract::Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.105163

    authors: Olson ML,Sandison ME,Chalmers S,McCarron JG

    更新日期:2012-11-15 00:00:00

  • CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair.

    abstract::Chromatin is no longer considered to be a static structural framework for packaging DNA within the nucleus but is instead believed to be an interactive component of DNA metabolism. The ordered assembly of chromatin produces a nucleoprotein template capable of epigenetically regulating the expression and maintenance of...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:

    authors: Ridgway P,Almouzni G

    更新日期:2000-08-01 00:00:00

  • Chemotactic activation of Dictyostelium AGC-family kinases AKT and PKBR1 requires separate but coordinated functions of PDK1 and TORC2.

    abstract::Protein kinases AKT and PKBR1 of Dictyostelium belong to the AGC protein kinase superfamily. AKT and PKBR1 are phosphorylated at similar sites by phosphoinositide-dependent kinase 1 (PDK1) and TORC2 kinases; however, they have different subcellular localizing domains. AKT has a phosphoinositide 3-kinase (PI3K)/phospha...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.064022

    authors: Liao XH,Buggey J,Kimmel AR

    更新日期:2010-03-15 00:00:00

  • An in vitro compartmental system underlines the contribution of mitochondrial immobility to the ATP supply in the NMJ.

    abstract::The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.234492

    authors: Altman T,Geller D,Kleeblatt E,Gradus-Perry T,Perlson E

    更新日期:2019-12-02 00:00:00

  • Junctional protein MAGI-3 interacts with receptor tyrosine phosphatase beta (RPTP beta) and tyrosine-phosphorylated proteins.

    abstract::Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00302

    authors: Adamsky K,Arnold K,Sabanay H,Peles E

    更新日期:2003-04-01 00:00:00

  • Association of mammalian SMC1 and SMC3 proteins with meiotic chromosomes and synaptonemal complexes.

    abstract::In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Eijpe M,Heyting C,Gross B,Jessberger R

    更新日期:2000-02-01 00:00:00