Abstract：
:Analysis by double-label indirect immunofluorescence of PtK1 and HeLa cells had previously demonstrated that prosome* antigens form networks that superimpose on those of the intermediate filaments of the cytokeratin type. We show here that in PtK1 cells various prosomal antigens also reside to a variable extent on intermediate filaments subnetworks of the vimentin type. In proliferating human fibroblasts the prosome and vimentin networks were found to coincide, while in proliferating myoblasts of the C2.7 mouse myogenic cell line the prosomal antigens seem to superimpose on the intermediate filaments of the desmin type. Thus, the prosomes, which are RNP particles of variable composition and subcomplexes of untranslated mRNP, and carry a multicatalytic proteinase activity, seem to co-localize with the specific kind of cytoplasmic intermediate filament in relation to the cell type. These results, which generalize the previous data, are discussed in view of possible role(s) for prosomes in mRNA metabolism and/or intermediate filaments remodelling.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Olink-Coux M,Arcangeletti C,Pinardi F,Minisini R,Huesca M,Chezzi C,Scherrer Ksubject
Has Abstractpub_date
1994-03-01 00:00:00pages
353-66eissn
0021-9533issn
1477-9137journal_volume
107 ( Pt 3)pub_type
杂志文章abstract：:Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migrati...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Stan...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Rabbit muscle myosin subfragment S1 decorates 6 nm diameter filaments in Drosophila wing epidermal cells in the arrowhead fashion characteristic of the binding of subfragment S1 to actin filaments. The filaments in question are concentrated between microtubules that are mostly composed of 15 protofilaments and form ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Vertebrate oocytes proceed through the first and the second meiotic division without an intervening S-phase to become haploid. Although DNA replication does not take place, unfertilized oocytes acquire the competence to replicate DNA one hour after the first meiotic division by accumulating an essential factor of the ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.166553
更新日期：2015-07-15 00:00:00
abstract：:The adapter protein CD2-associated protein (CD2AP) functions in various signaling and vesicle trafficking pathways, including endosomal sorting and/or trafficking and degradation pathways. Here, we investigated the role of CD2AP in insulin-dependent glucose transporter 4 (Glut4, also known as SLC2A4) trafficking and g...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.175075
更新日期：2015-12-15 00:00:00
abstract：:Recently, we demonstrated in cultured endothelial cells and in vivo that deficiency of an isoform of intersectin-1, ITSN-1s, impairs caveolae and clathrin-mediated endocytosis and functionally upregulates compensatory pathways and their morphological carriers (i.e. enlarged endocytic structures, membranous rings or tu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.163030
更新日期：2015-04-15 00:00:00
abstract：:Integrins are implicated in cell adhesion, migration and homeostasis. An important feature is their ability to adopt different affinity states that can be regulated by a variety of intra- and extracellular factors. To study affinity modulation of the integrin ectodomain by extracellular factors, we produced a soluble ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:The insulin receptor tyrosine kinase substrate p53 (IRSp53) links Rac and WAVE2 and has been implicated in lamellipodia protrusion. Recently, however, IRSp53 has been reported to bind to both Cdc42 and Mena to induce filopodia. To shed independent light on IRSp53 function we determined the localisations and dynamics o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00462
更新日期：2003-06-15 00:00:00
abstract：:p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:During anaphase-I in crane-fly spermatocytes, sister half-bivalents separate and move to opposite poles. When we irradiate a kinetochore spindle fibre with an ultraviolet microbeam, the associated half-bivalent temporarily stops moving and so does the partner half-bivalent with which it was paired during metaphase. To...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii, and found that it reacts with a single flagellar glycoprotein of about 230 kDa. Interestingly, immunofluorescence microscopy demonstra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:In some individuals with autosomal dominant isolated growth hormone deficiency, one copy of growth hormone lacks amino acids 32-71 and is severely misfolded. We transfected COS7 cells with either wild-type human growth hormone or Delta 32-71 growth hormone and investigated subcellular localization of growth hormone an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:The centrosome organizes microtubules by controlling nucleation and anchoring processes. In mammalian cells, subdistal appendages of the mother centriole are major microtubule-anchoring structures of the centrosome. It is not known how newly nucleated microtubules are anchored to these appendages. We show here that ni...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02302
更新日期：2005-04-15 00:00:00
abstract：:Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but rep...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033852
更新日期：2008-12-01 00:00:00
abstract：:The Cep63-Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear. In this study, we show that Cep57 and its paralog, Cep57l1, colocalize with Cep63 and Cep152 at the proximal end of mother centrioles in both c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.241836
更新日期：2020-07-03 00:00:00
abstract：:Gene expression of malaria parasites is mediated by the apicomplexan Apetala2 (ApiAP2) transcription factor family. Different ApiAP2s control gene expression at distinct stages in the complex life cycle of the parasite, ensuring timely expression of stage-specific genes. ApiAP2s recognize short cis-regulatory elements...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.210971
更新日期：2018-10-22 00:00:00
abstract：:Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.138313
更新日期：2013-11-15 00:00:00
abstract：:Intracellular pH has been measured by laser microspectrofluorimetry, using the pH-sensitive dyes SNARF-1, SNARF-calcein and SNARF-1-dextran. By this technique it was possible to accurately determine pH in volumes as small as 0.5 x 0.5 x 1 microns 3. The probes were loaded into the cells either by diffusion of their ac...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:CK2 is a serine/threonine kinase with many substrates, largely unknown modes of regulation and essential roles in mitotic progression. CK2α, a catalytic subunit of CK2, is phosphorylated in mitosis, and here we examine the effect of phosphorylation on CK2α localization. Using phosphospecific antibodies, we show that C...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.077446
更新日期：2011-07-15 00:00:00
abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.169367
更新日期：2015-09-15 00:00:00
abstract：:Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in se...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Cancer cells thrive when challenged with proteotoxic stress by inducing components of the protein folding, proteasome, autophagy and unfolded protein response (UPR) pathways. Consequently, specific molecular chaperones have been validated as targets for anti-cancer therapies. For example, inhibition of Hsp70 family pr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.217760
更新日期：2018-09-05 00:00:00
abstract：:Ca2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric oxide (NO) generation is dependent on Ca2+ and calmodulin (CaM). Here, we used Arabi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.201467
更新日期：2018-01-29 00:00:00
abstract：:The influence of extracellular environmental factors on preimplantation rat blastocysts was tested by determining the number of embryos which escaped from their zonae pellucidae, followed by attachment and outgrowth of trophoblast giant cells, after 72 h in culture Uterine luminal ocmponents from individual females, o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Rho GTPases and their downstream effectors regulate changes in the actin cytoskeleton that underlie cell motility and adhesion. They also participate, with RhoA, in the regulation of gene transcription by activating serum response factor (SRF)-mediated transcription from the serum response element (SRE). SRF-mediated ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.013557
更新日期：2008-02-15 00:00:00
abstract：:Rubella virus (RV) envelope glycoproteins E1 and E2 are targeted to the Golgi as heterodimers. While E2 contains a transmembrane Golgi retention signal, E1 is arrested in a pre-Golgi compartment in the absence of E2, and appears to require heterodimerization in order to reach the Golgi. Various forms of E1 with deleti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
