Human syntaxin 3 is localized apically in human intestinal cells.

abstract：:Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor ...

journal_title：Journal of cell science

authors： Prasad S,Ponimaskin E,Zeug A

更新日期：2019-08-23 00:00:00

abstract：:The specification of organs, tissues and cell types results from cell fate restrictions enacted by nuclear transcription factors under the control of conserved signaling pathways. The progenitor epithelium of the Drosophila compound eye, the eye imaginal disc, is a premier model for the study of such processes. Early ...

journal_title：Journal of cell science

authors： Neal SJ,Zhou Q,Pignoni F

更新日期：2020-05-26 00:00:00

abstract：:Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultane...

journal_title：Journal of cell science

authors： Chatre L,Ricchetti M

更新日期：2013-02-15 00:00:00

abstract：:Inactivation of the transcription factor and tumor suppressor p53, and overexpression or mutational activation of PIK3CA, which encodes the p110alpha catalytic subunit of phosphatidylinositol-3-kinase (PI3K), are two of the most common deleterious genomic changes in cancer, including in ovarian carcinomas. We investig...

journal_title：Journal of cell science

authors： Astanehe A,Arenillas D,Wasserman WW,Leung PC,Dunn SE,Davies BR,Mills GB,Auersperg N

更新日期：2008-03-01 00:00:00

abstract：:Tight junctions between endothelial cells of brain capillaries are the most important structural elements of the blood-brain barrier. Cultured brain endothelial cells are known to loose tight junction-dependent blood-brain barrier characteristics such as macromolecular impermeability and high electrical resistance. We...

journal_title：Journal of cell science

authors： Wolburg H,Neuhaus J,Kniesel U,Krauss B,Schmid EM,Ocalan M,Farrell C,Risau W

更新日期：1994-05-01 00:00:00

abstract：:An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing ev...

journal_title：Journal of cell science

authors： Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

更新日期：2010-09-01 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and intercellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how CME functions in planta To fac...

journal_title：Journal of cell science

authors： Johnson A,Gnyliukh N,Kaufmann WA,Narasimhan M,Vert G,Bednarek SY,Friml J

更新日期：2020-08-06 00:00:00

abstract：:Immobilization of cells by specific immune serum involves crosslinking between immunoglobulin G (IgG) and the i-antigen in the cell membrane. Globular material is seen to accumulate at the ciliary tips by phase-contrast and fluorescence microscopy in a manner analogous to 'capping' in more typical eukaryotes. When imm...

journal_title：Journal of cell science

authors： Barnett A,Steers E Jr

更新日期：1984-01-01 00:00:00

abstract：:In the eukaryotic cell, a protein quality control process termed endoplasmic reticulum-associated degradation (ERAD) rids the ER of aberrant proteins and unassembled components of protein complexes that fail to reach a transport-competent state. To identify novel genes required for ERAD, we devised a rapid immunoassay...

journal_title：Journal of cell science

authors： Palmer EA,Kruse KB,Fewell SW,Buchanan SM,Brodsky JL,McCracken AA

更新日期：2003-06-01 00:00:00

abstract：:We previously demonstrated that activation of v-ABL protein tyrosine kinase resulted in suppression of apoptosis following interleukin-3 removal using an interleukin-3-dependent haemopoietic cell line transfected with a temperature-sensitive mutant of the v-abl oncoprotein (IC.DP). Cellular signalling events associate...

journal_title：Journal of cell science

authors： Evans CA,Lord JM,Owen-Lynch PJ,Johnson G,Dive C,Whetton AD

更新日期：1995-07-01 00:00:00

abstract：:Occludin and 18 distinct members of the claudin family are tetra-span transmembrane proteins that are localized in cell-specific tight junctions (TJs). A previous study showed that expression of chick occludin in Madin-Darby canine kidney (MDCK) cells raised transepithelial electrical resistance (TER) and, paradoxical...

journal_title：Journal of cell science

authors： McCarthy KM,Francis SA,McCormack JM,Lai J,Rogers RA,Skare IB,Lynch RD,Schneeberger EE

更新日期：2000-10-01 00:00:00

abstract：:p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 ...

journal_title：Journal of cell science

authors： Haskell MD,Nickles AL,Agati JM,Su L,Dukes BD,Parsons SJ

更新日期：2001-05-01 00:00:00

abstract：:The mechanism responsible for spermatid translocation in the mammalian seminiferous epithelium was proposed to be the microtubule-based transport of specialized junction plaques (ectoplasmic specializations) that occur in Sertoli cell regions attached to spermatid heads. These plaques each consist of a cistern of endo...

journal_title：Journal of cell science

authors： Guttman JA,Kimel GH,Vogl AW

更新日期：2000-06-01 00:00:00

abstract：:How signaling domains form is an important, yet largely unexplored question. Here, we show that ciliary proteins help establish two contiguous, yet distinct cyclic GMP (cGMP) signaling compartments in Caenorhabditis elegans thermosensory AFD neurons. One compartment, a bona fide cilium, is delineated by proteins assoc...

journal_title：Journal of cell science

authors： Nguyen PA,Liou W,Hall DH,Leroux MR

更新日期：2014-12-15 00:00:00

abstract：:The p53 tumor suppressor protein is a key regulator of cellular functions including responses to numerous stress signals, and triggers apoptosis in many cell types, including neurons. The major mechanisms known to regulate p53 stabilization and activation include phosphorylation and ubiquitin ligase-mediated proteasom...

journal_title：Journal of cell science

authors： Lee JH,Kim HS,Lee SJ,Kim KT

更新日期：2007-07-01 00:00:00

abstract：:BCL6 is a potent transcriptional repressor that plays important roles in germinal center formation, T helper cell differentiation and lymphomagenesis and regulates expression of several chemokine genes in macrophages. In a further investigation of its role in macrophages, we show that BCL6 inactivation in primary bone...

journal_title：Journal of cell science

authors： Pixley FJ,Xiong Y,Yu RY,Sahai EA,Stanley ER,Ye BH

更新日期：2005-05-01 00:00:00

abstract：:Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...

journal_title：Journal of cell science

authors： Shinohara M,Hayashihara K,Grubb JT,Bishop DK,Shinohara A

更新日期：2015-04-15 00:00:00

abstract：:Casein kinase 1 (CK1) plays central roles in various signal transduction pathways and performs many cellular activities. For many years CK1 was thought to act independently of modulatory subunits and in a constitutive manner. Recently, DEAD box RNA helicases, in particular DEAD box RNA helicase 3 X-linked (DDX3X), wer...

journal_title：Journal of cell science

authors： Dolde C,Bischof J,Grüter S,Montada A,Halekotte J,Peifer C,Kalbacher H,Baumann U,Knippschild U,Suter B

更新日期：2018-01-04 00:00:00

abstract：:The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress. We have used a cellular model of conditionally...

journal_title：Journal of cell science

authors： Bravo M,Nicolini F,Starowicz K,Barroso S,Calés C,Aguilera A,Vidal M

更新日期：2015-10-01 00:00:00

abstract：:Atomic force microscopy (AFM) was used to probe topology, conformational changes and initial substrate-carrier interactions of Na+-glucose co-transporter (SGLT1) in living cells on a single-molecule level. By scanning SGLT1-transfected Chinese hamster ovary (CHO) cells with AFM tips carrying an epitope-specific antibo...

journal_title：Journal of cell science

authors： Puntheeranurak T,Wildling L,Gruber HJ,Kinne RK,Hinterdorfer P

更新日期：2006-07-15 00:00:00

abstract：:The semaphorin family comprises soluble and membrane-bound proteins originally identified as axonal guidance cues that function during neuronal development. Emerging evidence suggests that a subset of semaphorins, called 'immune semaphorins', function in the immune system. The class IV semaphorins Sema4D/CD100 and Sem...

journal_title：Journal of cell science

authors： Kumanogoh A,Kikutani H

更新日期：2003-09-01 00:00:00

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:Microtubules--polymers of tubulin--perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications of tubulin subunits diversify the outer and luminal ...

journal_title：Journal of cell science

authors： Wloga D,Gaertig J

更新日期：2010-10-15 00:00:00

abstract：:Loss-of-function mutations in the genes encoding PINK1 and Parkin (also known as PARK2) are the most common causes of recessive Parkinson's disease. Both together mediate the selective degradation of mitochondrial proteins and whole organelles via the proteasome and the autophagy-lysosome pathway (mitophagy). The mito...

journal_title：Journal of cell science

authors： Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

更新日期：2014-08-15 00:00:00

abstract：:Cartilage oligomeric matrix protein (COMP) is an abundant component in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and ...

journal_title：Journal of cell science

authors： Schulz JN,Nüchel J,Niehoff A,Bloch W,Schönborn K,Hayashi S,Kamper M,Brinckmann J,Plomann M,Paulsson M,Krieg T,Zaucke F,Eckes B

更新日期：2016-02-15 00:00:00

abstract：:MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 b...

journal_title：Journal of cell science

authors： Scales TM,Lin S,Kraus M,Goold RG,Gordon-Weeks PR

更新日期：2009-07-15 00:00:00

abstract：:Reorganization of the actin cytoskeleton is essential to numerous cellular processes including cell locomotion and cytokinesis. This actin remodeling is regulated in part by Rho family GTPases. Previous studies implicated Trio, a Dbl-homology guanine nucleotide exchange factor with two exchange factor domains, in regu...

journal_title：Journal of cell science

authors： Seipel K,O'Brien SP,Iannotti E,Medley QG,Streuli M

更新日期：2001-01-01 00:00:00

abstract：:A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...

journal_title：Journal of cell science

authors： Hecht RM,Berg-Zabelshansky M,Rao PN,Davis FM

更新日期：1987-03-01 00:00:00

abstract：:Recently, we demonstrated in cultured endothelial cells and in vivo that deficiency of an isoform of intersectin-1, ITSN-1s, impairs caveolae and clathrin-mediated endocytosis and functionally upregulates compensatory pathways and their morphological carriers (i.e. enlarged endocytic structures, membranous rings or tu...

journal_title：Journal of cell science

authors： Bardita C,Predescu DN,Sha F,Patel M,Balaji G,Predescu SA

更新日期：2015-04-15 00:00:00

abstract：:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...

journal_title：Journal of cell science

authors： Paladino S,Lebreton S,Tivodar S,Campana V,Tempre R,Zurzolo C

更新日期：2008-12-15 00:00:00