Abstract：
:MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 beta sites that we had previously characterised. We raised a panel of phosphospecific antibodies to these sites on MAP1B and used it to assess the distribution of phosphorylated MAP1B in the developing nervous system. This showed that the nonprimed sites are restricted to growing axons, whereas the primed sites are also expressed in the neuronal cell body. To identify kinases phosphorylating MAP1B, we added kinase inhibitors to cultured embryonic cortical neurons and monitored MAP1B phosphorylation with our panel of phosphospecific antibodies. These experiments identified dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK1A) as the kinase that primes sites of GSK3 beta phosphorylation in MAP1B, and we confirmed this by knocking down DYRK1A in cultured embryonic cortical neurons by using shRNA. DYRK1A knockdown compromised neuritogenesis and was associated with alterations in microtubule stability. These experiments demonstrate that MAP1B has DYRK1A-primed and nonprimed GSK3 beta sites that are involved in the regulation of microtubule stability in growing axons.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Scales TM,Lin S,Kraus M,Goold RG,Gordon-Weeks PRdoi
10.1242/jcs.040162subject
Has Abstractpub_date
2009-07-15 00:00:00pages
2424-35issue
Pt 14eissn
0021-9533issn
1477-9137pii
jcs.040162journal_volume
122pub_type
杂志文章abstract：:We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10-15% of sera from patients with anti-centromere...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a poss...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01726
更新日期：2005-03-15 00:00:00
abstract：:Several examples of direct interactions between helicases and topoisomerases have recently been described. The data suggest a possible cooperation between these enzymes in major DNA events such as the progression of a replication fork, segregation of newly replicated chromosomes, disruption of nucleosomal structure, D...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：1997-06-01 00:00:00
abstract：:We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Arf GTPases regulate both the morphological and protein sorting events that are essential for membrane trafficking. Guanine nucleotide exchange factors (GEFs) specific for Arf proteins determine when and where Arf GTPases will be activated in cells. The yeast Gea2p Arf GEF is a member of an evolutionarily conserved fa...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00896
更新日期：2004-02-15 00:00:00
abstract：:Different isoforms of non-muscle myosin II have different distributions in vivo, even within individual cells. In order to understand how these different distributions arise, the distribution and dynamics of non-muscle myosins IIA and myosin IIB were examined in cultured cells using immunofluorescence staining and tim...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:Heparin-binding EGF-like growth factor (HB-EGF) is a ligand for EGF receptor (EGFR) and possesses the ability to signal in juxtacrine, autocrine and/or paracrine mode, with these alternatives being governed by the degree of proteolytic release of the ligand. Although the spatial range of diffusion of released HB-EGF i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.058321
更新日期：2010-07-01 00:00:00
abstract：:Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril assembly, but the mechanism of actin regulation in striated muscle is ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.104950
更新日期：2012-09-01 00:00:00
abstract：:Desmoplakin is an intracellular component of desmosomes which plays a role in the anchorage of intermediate filaments to these structures. We report here that, despite the absence of desmosomes, cultured endothelial cells from human umbilical vein express desmoplakin I and II both at mRNA and protein level. Desmoplaki...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:The peroxin Pex11 has a recognized role in peroxisome division. Pex11p remodels and elongates peroxisomal membranes prior to the recruitment of dynamin-related GTPases that act in membrane scission to divide peroxisomes. We performed a comprehensive comparative genomics survey to understand the significance of the evo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.157743
更新日期：2015-04-01 00:00:00
abstract：:Lysosomal cysteine proteases from mammalian cells and plants are regulated by endogenous tight-binding inhibitors from the cystatin superfamily. The presence of cystatin-like inhibitors in lower eukaryotes such as protozoan parasites has not yet been demonstrated, although these cells express large quantities of cyste...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00302
更新日期：2003-04-01 00:00:00
abstract：:TANGLED (TAN) is the founding member of a family of plant-specific proteins required for correct orientation of the division plane. Arabidopsis thaliana TAN is localized before prophase until the end of cytokinesis at the cortical division site (CDS), where it appears to help guide the cytokinetic apparatus towards th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.073676
更新日期：2011-01-15 00:00:00
abstract：:Neural stem cells (NSCs) typically show efficient self-renewal and selective differentiation. Their invasion potential, however, is not well studied. In this study, Sox2-positive NSCs from the E14.5 rat cortex were found to be non-invasive and showed only limited migration in vitro. By contrast, FGF2-expanded NSCs sho...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.125757
更新日期：2013-08-15 00:00:00
abstract：:We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-12-01 00:00:00
abstract：:Calmodulin was localized in the small intestine of Necturus maculosa at both the light- and electron-microscopic levels utilizing an affinity-purified, monospecific antibody and immunoperoxidase cytochemistry. The microvilli and basolateral membranes of the epithelium were highly reactive, the cytoplasm was moderately...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.087965
更新日期：2011-08-01 00:00:00
abstract：:Previously, we demonstrated that proteolytic processing within the globular domain of the alpha3 subunit of laminin-5 (LN5) converts LN5 from a cell motility-inducing factor to a protein complex that can trigger the formation of hemidesmosomes, certain cell-matrix attachment sites found in epithelial cells. We have pr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:FAS associated factor 1 (FAF1) has been described as an unusual protein that binds to the intracellular portion of the apoptosis signal transducing receptor FAS/Apo-1 and potentiates apoptosis in L-cells. By means of mRNA differential display we have identified the avian homologue (qFAF) as a fibroblast growth factor-...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:Yeast cells were cultivated at different growth rates in a chemostat by alterations in the flow of the limiting nutrient glucose and in batch cultures where variations in growth rate were achieved by alterations in the composition of nutrients. It was observed that the stage in the cycle at which S-phase was completed...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Previous investigators have examined mitochondrial DNA (mtDNA) in the electron microscope (EM) after extraction from mitochondria and rotary shadowing. We have observed mtDNA in situ by the osmiumammine procedure for specific staining of DNA in the EM. The procedure was modified to improve the regularity of the staini...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Morphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conv...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The transbilayer dynamics of lipids in the plasma membrane of mammalian sperm cells is crucial for the fertilization process. Here, the transbilayer movement and distribution of phospholipids in the plasma membrane of fresh, ejaculated and cryopreserved ram spermatozoa was studied by labeling cells with fluorescent an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Syndecan-4 is a ubiquitously expressed transmembrane heparan sulphate proteoglycan acting in concert with integrins in the formation of focal adhesions and stress fibres. Signalling events studied thus far suggest the formation of a ternary complex between syndecan-4, phosphatidylinositol 4,5-bisphosphate and protein ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03020
更新日期：2006-07-01 00:00:00
abstract：:Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.230508
更新日期：2019-07-18 00:00:00
abstract：:The outer nuclear membrane proteins nesprin-1 and nesprin-2 are retained at the nuclear envelope through an interaction of their klarsicht/ANC-1/syne homology (KASH) domain with Sun proteins present at the inner nuclear membrane. We investigated the requirements for the localization of nesprin-3alpha at the outer nucl...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.014191
更新日期：2007-10-01 00:00:00
abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.146316
更新日期：2014-05-15 00:00:00
