Abstract:
:In the eukaryotic cell, a protein quality control process termed endoplasmic reticulum-associated degradation (ERAD) rids the ER of aberrant proteins and unassembled components of protein complexes that fail to reach a transport-competent state. To identify novel genes required for ERAD, we devised a rapid immunoassay to screen yeast lacking uncharacterized open reading frames that were known targets of the unfolded protein response (UPR), a cellular response that is induced when aberrant proteins accumulate in the ER. Six genes required for the efficient degradation of the Z variant of the alpha1-proteinase inhibitor (A1PiZ), a known substrate for ERAD, were identified, and analysis of other ERAD substrates in the six A1PiZ-degradation-deficient (add) mutants suggested diverse requirements for the Add proteins in ERAD. Finally, we report on bioinformatic analyses of the new Add proteins, which will lead to testable models to elucidate their activities.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Palmer EA,Kruse KB,Fewell SW,Buchanan SM,Brodsky JL,McCracken AAdoi
10.1242/jcs.00439keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
2361-73issue
Pt 11eissn
0021-9533issn
1477-9137pii
jcs.00439journal_volume
116pub_type
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