Abstract:
:Shape changes have been determined in human blood lymphocytes stimulated with OAG, diC8, PMA, colchicine or the hexapeptide fNLPNTL in short-term assays (30 min). Distinct types of shape-change responses were observed. Colchicine was active in generating a relatively small proportion of polarized lymphocytes (front-tail polarity). OAG, diC8 and PMA produced different types of shape change (non-polar cells with surface projections), and these were closely associated with an increase in actin polymerization and a shift of F-actin into the projections at the cell periphery. The diacylglycerols OAG and diC8 produced biphasic dose-response curves leading to rounding up of cells at very high stimulant concentrations. PMA produced no comparable biphasic response when tested over a much wider concentration range. Though the nonpolar cells with surface projections generated by OAG, diC8 or PMA showed vigorous shape changes, they lacked significant locomotor activity. alpha-Phorbol, 4 alpha-PDD, lumicolchicine or fNLPNTL were inactive. Small blood lymphocytes stimulated by OAG, diC8 or PMA showed a very small increase in the net uptake of FITC-dextran by fluid pinocytosis. Unlike neutrophils, which show a high net uptake, lymphocytes did not concentrate FITC-dextran in large granules, indicating that they do not develop a 'storage' compartment in the form of large vesicles. However, small fluorescent spots were consistently found in at least a fraction of blood lymphocytes. The results indicate that stimulated surface movement may be instrumental in fluid pinocytosis. Diacylglycerols may act as second messengers to induce pinocytosis, shape changes and altered actin polymerization in lymphocytes.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Keller HU,Niggli V,Zimmermann Asubject
Has Abstractpub_date
1989-07-01 00:00:00pages
457-65eissn
0021-9533issn
1477-9137journal_volume
93 ( Pt 3)pub_type
杂志文章abstract::The mechanisms by which volatile general anaesthetics (VAs) produce a depression of central nervous system are beginning to be better understood, but little is known about a number of side effects. Here, we show that the cold receptor transient receptor potential melastatin 8 (TRPM8) undergoes a complex modulation by ...
journal_title:Journal of cell science
pub_type: 杂志文章
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abstract::The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...
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pub_type: 杂志文章
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2006-12-01 00:00:00
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更新日期:2001-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2004-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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更新日期:2017-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2019-02-11 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00377
更新日期:2003-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2004-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.039065
更新日期:2008-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:1998-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2006-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2000-12-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2015-03-01 00:00:00
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pub_type: 评论,杂志文章,评审
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更新日期:2001-10-01 00:00:00