Control of mitochondrial homeostasis by endocytic regulatory proteins.

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...

journal_title：Journal of cell science

authors： Malínsky J,Koberna K,Stanĕk D,Masata M,Votruba I,Raska I

更新日期：2001-02-01 00:00:00

abstract：:Candida albicans is a dimorphic fungus capable of growing as a budding yeast and as a filamentous hypha. We have used the technique of immunofluorescence to study the changes in the microtubule cytoskeleton during the cell cycle in both growth forms. This approach has revealed the presence of an extensive system of mi...

journal_title：Journal of cell science

authors： Barton R,Gull K

更新日期：1988-10-01 00:00:00

abstract：:The lipid phosphate phosphatase-related proteins (LPPRs), also known as plasticity-related genes (PRGs), are classified as a new brain-enriched subclass of the lipid phosphate phosphatase (LPP) superfamily. They induce membrane protrusions, neurite outgrowth or dendritic spine formation in cell lines and primary neuro...

journal_title：Journal of cell science

authors： Yu P,Agbaegbu C,Malide DA,Wu X,Katagiri Y,Hammer JA,Geller HM

更新日期：2015-09-01 00:00:00

abstract：:Dyneins are motor proteins that move along microtubules. They have many roles in the cell. They drive the beating of cilia and flagella, move cargos in the cytoplasm and function in the mitotic spindle. Dyneins are large and complex protein machines. Until recently, the way they move was poorly understood. In 2012, tw...

journal_title：Journal of cell science

authors： Carter AP

更新日期：2013-02-01 00:00:00

abstract：:Na+/H+ exchangers (NHEs) are transporters that exchange sodium and proton ions across the plasma membrane at the expense of their chemical gradient. In higher eukaryotes these transporters exist as multiple specialized isoforms. For example, NHE1, the ubiquitously expressed form is a major pH-regulating system whereas...

journal_title：Journal of cell science

authors： Noel J,Roux D,Pouysségur J

更新日期：1996-05-01 00:00:00

abstract：:The mAb RK7, previously shown to recognize keratin 19, was also found to cross-react with a biologically unrelated 102 kDa protein, which becomes associated with the poles of the mitotic apparatus. This newly identified protein, called cytocentrin, is a stable cellular component, may be at least in part phosphorylated...

journal_title：Journal of cell science

authors： Paul EC,Quaroni A

更新日期：1993-11-01 00:00:00

abstract：:Stressed cells downregulate translation initiation and assemble membrane-less foci termed stress granules (SGs). Although SGs have been extensively characterized in cultured cells, the existence of such structures in stressed adult stem cell pools remains poorly characterized. Here, we report that the Drosophila ortho...

journal_title：Journal of cell science

authors： Buddika K,Ariyapala IS,Hazuga MA,Riffert D,Sokol NS

更新日期：2020-05-18 00:00:00

abstract：:The scaffold attachment factor SAFB1 and its recently discovered homologue SAFB2 might provide an important link between pre-mRNA splicing, intracellular signalling and transcription. Using novel mono-specific antisera, we found endogenous SAFB2 protein has a different spatial distribution from SAFB1 within the nucleu...

journal_title：Journal of cell science

authors： Sergeant KA,Bourgeois CF,Dalgliesh C,Venables JP,Stevenin J,Elliott DJ

更新日期：2007-01-15 00:00:00

abstract：:The development or maturation of intercellular adhesions following their initiation has received very little attention even though this is an area of significance for a variety of in vivo processes. Using Ca2(+)-induced desmosome formation in MDCK cells as a study system it is shown that, following its initiation, des...

journal_title：Journal of cell science

authors： Mattey DL,Burdge G,Garrod DR

更新日期：1990-12-01 00:00:00

abstract：:Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tig...

journal_title：Journal of cell science

authors： Murphy CR,Martin B

更新日期：1985-10-01 00:00:00

abstract：:Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...

journal_title：Journal of cell science

authors： Singh RK,Lund FW,Haka AS,Maxfield FR

更新日期：2019-12-02 00:00:00

abstract：:Small latent TGF-beta consists of latency associated peptide (LAP) bound to the 25 kDa TGF-beta by noncovalent interactions. Small latent TGF-beta is secreted from cells and deposited into the extracellular matrix as covalent complexes with its binding proteins, LTBPs. Four LTBPs have been molecularly cloned and their...

journal_title：Journal of cell science

authors： Koli K,Saharinen J,Kärkkäinen M,Keski-Oja J

更新日期：2001-08-01 00:00:00

abstract：:The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed...

journal_title：Journal of cell science

authors： Diestel S,Schaefer D,Cremer H,Schmitz B

更新日期：2007-11-15 00:00:00

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the beta-oxidation of fatty acids. Characterization of their properties and behavior has progressed rather slowly. However, during ...

journal_title：Journal of cell science

authors： van der Zand A,Braakman I,Geuze HJ,Tabak HF

更新日期：2006-03-15 00:00:00

abstract：:Male infertility has become a worldwide health problem, but the etiologies of most cases are still unknown. SEPT12, a GTP-binding protein, is involved in male fertility. Two SEPT12 mutations (SEPT12(T89M) and SEPT12(D197N)) have been identified in infertile men who have a defective sperm annulus with a bent tail. The ...

journal_title：Journal of cell science

authors： Kuo YC,Shen YR,Chen HI,Lin YH,Wang YY,Chen YR,Wang CY,Kuo PL

更新日期：2015-03-01 00:00:00

abstract：:Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode de...

journal_title：Journal of cell science

authors： Jabbour AM,Puryer MA,Yu JY,Lithgow T,Riffkin CD,Ashley DM,Vaux DL,Ekert PG,Hawkins CJ

更新日期：2006-06-15 00:00:00

abstract：:Connexin 30 (Cx30), a member of the large gap-junction protein family, plays a role in the homeostasis of the epidermis and inner ear through gap junctional intercellular communication (GJIC). Here, we investigate the underlying mechanisms of four autosomal dominant Cx30 gene mutations that are linked to hearing loss ...

journal_title：Journal of cell science

authors： Berger AC,Kelly JJ,Lajoie P,Shao Q,Laird DW

更新日期：2014-04-15 00:00:00

abstract：:The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones isolated from a HeLa cell cDNA library and confirmed by a combination of comparison to expressed sequence tags, microsequencing of purified protein, and in vitro transcription and trans...

journal_title：Journal of cell science

authors： Singleton DR,Wu TT,Castle JD

更新日期：1997-09-01 00:00:00

abstract：:The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubuli...

journal_title：Journal of cell science

authors： Diggins-Gilicinski M,Solnica-Krezel L,Burland TG,Paul EC,Dove WF

更新日期：1989-10-01 00:00:00

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:Besides regulating glucose levels, insulin has been reported to participate actively in many other functions, including modulating inflammatory reactions. In this study we investigated how topical insulin application would affect the diabetic wound healing process. We found that the excessive expression of insulin-deg...

journal_title：Journal of cell science

authors： Yang P,Wang X,Wang D,Shi Y,Zhang M,Yu T,Liu D,Gao M,Zhang X,Liu Y

更新日期：2020-10-12 00:00:00

abstract：:Entry into mitosis requires p34(cdc2), which activates downstream mitotic events through phosphorylation of key target proteins. In Aspergillus nidulans, the NIMA protein kinase has been identified as a potential downstream target and plays a role in regulating chromatin condensation at mitosis. nimA- mutants arrest i...

journal_title：Journal of cell science

authors： Krien MJ,Bugg SJ,Palatsides M,Asouline G,Morimyo M,O'Connell MJ

更新日期：1998-04-01 00:00:00

abstract：:Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during migration over the provisional matrix. To investigate the function of the laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cr...

journal_title：Journal of cell science

authors： Margadant C,Raymond K,Kreft M,Sachs N,Janssen H,Sonnenberg A

更新日期：2009-01-15 00:00:00

abstract：:Here, we identify a role for the matrilin-2 (Matn2) extracellular matrix protein in controlling the early stages of myogenic differentiation. We observed Matn2 deposition around proliferating, differentiating and fusing myoblasts in culture and during muscle regeneration in vivo. Silencing of Matn2 delayed the express...

journal_title：Journal of cell science

authors： Deák F,Mátés L,Korpos E,Zvara A,Szénási T,Kiricsi M,Mendler L,Keller-Pintér A,Ozsvári B,Juhász H,Sorokin L,Dux L,Mermod N,Puskás LG,Kiss I

更新日期：2014-08-01 00:00:00

abstract：:For all eukaryotic cilia the basal bodies provide a template for the assembly of the doublet microtubules, and intraflagellar transport provides a mechanism for transport of axonemal components into the growing cilium. What is not known is how the central pair of microtubules is nucleated or how their associated polyp...

journal_title：Journal of cell science

authors： Dymek EE,Smith EF

更新日期：2012-07-15 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:Previous studies have shown that the non-alpha-helical, amino-terminal head region of vimentin is essential for the formation and stability of vimentin intermediate filaments (IFs). In order to specify its target site on companion protein subunits, it was cut off from vimentin at amino acid position 96 with lysine-spe...

journal_title：Journal of cell science

authors： Traub P,Scherbarth A,Wiegers W,Shoeman RL

更新日期：1992-02-01 00:00:00

abstract：:We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that b...

journal_title：Journal of cell science

authors： Araki N,Hatae T,Yamada T,Hirohashi S

更新日期：2000-09-01 00:00:00