Abstract：
:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, in human keratinocytes, αVβ5 is predominantly found in FCLs, and formation of the αVβ5-containing FCLs requires the presence of vitronectin as ligand, Ca2+, and the clathrin adaptor proteins ARH (also known as LDLRAP1), Numb and EPS15/EPS15L1. Integrin chimeras, containing the extracellular and transmembrane domains of β5 and the cytoplasmic domains of β1 or β3, almost exclusively localize in FAs. Interestingly, lowering actomyosin-mediated contractility promotes integrin redistribution to FLCs in an integrin tail-dependent manner, while increasing cellular tension favors αVβ5 clustering in FAs. Our findings strongly indicate that clustering of integrin αVβ5 in FCLs is dictated by the β5 subunit cytoplasmic domain, cellular tension and recruitment of specific adaptor proteins to the β5 subunit cytoplasmic domains.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg Adoi
10.1242/jcs.221317subject
Has Abstractpub_date
2018-11-05 00:00:00issue
21eissn
0021-9533issn
1477-9137pii
jcs.221317journal_volume
131pub_type
杂志文章abstract：:FAS associated factor 1 (FAF1) has been described as an unusual protein that binds to the intracellular portion of the apoptosis signal transducing receptor FAS/Apo-1 and potentiates apoptosis in L-cells. By means of mRNA differential display we have identified the avian homologue (qFAF) as a fibroblast growth factor-...
journal_title：Journal of cell science
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更新日期：1998-08-01 00:00:00
abstract：:Mammalian cochlear outer hair cells generate high-frequency forces in response to electrical stimulation. Force generation occurs in the lateral cortex of the cell, which includes the plasma membrane, a two-dimensional 'cortical lattice' of filamentous protein, and a multi-layered membrane system, the lateral cisterna...
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abstract：:The adaptor protein SETA/CIN85/Ruk is involved in regulating diverse signal transduction pathways, including the internalization of tyrosine kinase receptors via the Cbl ubiquitin ligases, and attenuating PI3K activity by interaction with its regulatory subunit. Here we present evidence for a new aspect of SETA functi...
journal_title：Journal of cell science
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doi：10.1242/jcs.00522
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abstract：:Adaptive immunity, a vertebrate specialization, adds memory and exquisite specificity to the basic innate immune responses present in invertebrates while conserving metabolic resources. In adaptive immunity, antigenic challenge requires extremely rapid proliferation of rare antigen-specific lymphocytes to produce larg...
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abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...
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pub_type： 杂志文章
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：2010-03-15 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Journal of cell science
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更新日期：2000-11-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1996-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2003-05-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：2001-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01153
更新日期：2004-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
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doi：
更新日期：1989-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1994-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1991-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
