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CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells.

Abstract：

:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by phosphorylation by Cdk. CDC6 silencing by siRNAs affected ATR-dependent inhibition of mitotic entry elicited by modest replication stress. Whereas a Cdk-phosphorylation-mimicking CDC6 mutant could rescue the checkpoint defect by CDC6 silencing, a phosphorylation-deficient mutant could not. Furthermore, we found that the CDC6-ATR interaction is conserved in Xenopus. We show that the presence of Xenopus CDC6 during S phase is essential for Xenopus ATR to bind to chromatin in response to replication inhibition. In addition, when human CDC6 amino acid fragment 180-220, which can bind to both human and Xenopus ATR, was added to Xenopus egg extracts after assembly of the pre-replication complex, Xenopus Chk1 phosphorylation was significantly reduced without lowering replication, probably through a sequestration of CDC6-mediated ATR-chromatin interaction. Thus, CDC6 might regulate replication-checkpoint activation through the interaction with ATR in higher eukaryotic cells.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

doi

10.1242/jcs.058693

subject

Has Abstract

pub_date

2010-01-15 00:00:00

pages

225-35

issue

Pt 2

eissn

0021-9533

issn

1477-9137

pii

123/2/225

journal_volume

123

pub_type

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