Abstract：
:To optimally perform the diversity of metabolic functions that occur within peroxisomes, cells must dynamically regulate peroxisome size, number and content in response to the cell state and the environment. Except for transcriptional regulation little is known about the mechanisms used to perform this complicated feat. Focusing on the yeast Saccharomyces cerevisiae, we used complementary high-content screens to follow changes in localization of most proteins during growth in oleate. We found extensive changes in cellular architecture and identified several proteins that colocalized with peroxisomes that had not previously been considered peroxisomal proteins. One of the newly identified peroxisomal proteins, Ymr018w, is a protein with an unknown function that is similar to the yeast and human peroxisomal targeting receptor Pex5. We demonstrate that Ymr018w is a new peroxisomal-targeting receptor that targets a subset of matrix proteins to peroxisomes. We, therefore, renamed Ymr018w, Pex9, and suggest that Pex9 is a condition-specific targeting receptor that enables the dynamic rewiring of peroxisomes in response to metabolic needs. Moreover, we suggest that Pex5-like receptors might also exist in vertebrates.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Yifrach E,Chuartzman SG,Dahan N,Maskit S,Zada L,Weill U,Yofe I,Olender T,Schuldiner M,Zalckvar Edoi
10.1242/jcs.195255subject
Has Abstractpub_date
2016-11-01 00:00:00pages
4067-4075issue
21eissn
0021-9533issn
1477-9137pii
jcs.195255journal_volume
129pub_type
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