Abstract：
:While the basic cellular contributions to bone differentiation and mineralization are widely accepted, the regulation of these processes at the intracellular level remains inadequately understood. Our laboratory recently identified annexin 2 as a protein involved in osteoblastic mineralization. Annexin 2 was overexpressed twofold in SaOSLM2 osteoblastic cells as a fusion protein with green fluorescent protein. The overexpression of annexin 2 led to an increase in alkaline phosphatase activity as well as an increase in mineralization. Our data suggest that the increase in alkaline phosphatase activity does not result from increased alkaline phosphatase transcript or protein levels; therefore we evaluated mechanism of action. We determined that both annexin 2 and alkaline phosphatase activity were localized to membrane microdomains called lipid rafts in osteoblastic cells. Annexin 2 overexpression resulted in an increase in alkaline phosphatase activity that was associated with lipid microdomains in a cholesterol-dependent manner. Furthermore, disruption of lipid rafts with a cholesterol sequestering agent or reduction of annexin 2 expression by specific antisense oligonucleotides each resulted in diminished mineralization. Therefore, intact lipid rafts containing annexin 2 appear to be important for alkaline phosphatase activity and may facilitate the osteoblastic mineralization process.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Gillette JM,Nielsen-Preiss SMdoi
10.1242/jcs.00909keywords:
subject
Has Abstractpub_date
2004-01-26 00:00:00pages
441-9issue
Pt 3eissn
0021-9533issn
1477-9137pii
jcs.00909journal_volume
117pub_type
杂志文章abstract：:Intracellular pools of the heterotrimeric G-protein α-subunit Gαi3 (encoded by GNAI3) have been shown to promote growth factor signaling, while at the same time inhibiting the activation of JNK and autophagic signaling following nutrient starvation. The precise molecular mechanisms linking Gαi3 to both stress and grow...
journal_title：Journal of cell science
pub_type： 杂志文章
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abstract：:A UV-damaged DNA binding protein (UV-DDB) is the major source of UV-damaged DNA binding activity in mammalian cell extracts. This activity is defective in at least some xeroderma pigmentosum group E (XP-E) patients; microinjection of the UV-DDB protein into their fibroblasts corrects nucleotide excision repair (NER). ...
journal_title：Journal of cell science
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doi：
更新日期：1997-05-01 00:00:00
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journal_title：Journal of cell science
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abstract：:The adapter protein CD2-associated protein (CD2AP) functions in various signaling and vesicle trafficking pathways, including endosomal sorting and/or trafficking and degradation pathways. Here, we investigated the role of CD2AP in insulin-dependent glucose transporter 4 (Glut4, also known as SLC2A4) trafficking and g...
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
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更新日期：2018-04-12 00:00:00
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更新日期：2009-08-15 00:00:00
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更新日期：2018-02-22 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2020-11-03 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2007-04-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2010-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2012-06-15 00:00:00
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更新日期：2017-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1991-10-01 00:00:00
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