Abstract：
:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolaterally, respectively, in MDCK cells. We investigated the behaviour of the resulting fusion proteins GFP-FR and GFP-PrP by analysing three parameters: their association with DRMs, their oligomerisation and their apical sorting. Strikingly, we found that different GPI-attachment signals differently modulate the ability of the resulting GFP-fusion protein to oligomerise and to be apically sorted. This is probably owing to differences in the GPI anchor and/or in the surrounding lipid microenvironment. Accordingly, we show that addition of cholesterol to the cells is necessary and sufficient to drive the oligomerisation and consequent apical sorting of GFP-PrP, which under control conditions does not oligomerise and is basolaterally sorted.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Paladino S,Lebreton S,Tivodar S,Campana V,Tempre R,Zurzolo Cdoi
10.1242/jcs.036038subject
Has Abstractpub_date
2008-12-15 00:00:00pages
4001-7issue
Pt 24eissn
0021-9533issn
1477-9137pii
121/24/4001journal_volume
121pub_type
杂志文章abstract：:The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In...
journal_title：Journal of cell science
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更新日期：1976-12-01 00:00:00
abstract：:Angiogenesis is critical for vascular remodeling during development and contributes to the pathogenesis of diseases such as cancer. Targeted disruption of several EphB class receptor tyrosine kinases results in vascular remodeling defects during embryogenesis. The role of EphA class receptors in vascular remodeling, h...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2004-04-15 00:00:00
abstract：:Localization and translation of mRNAs within different subcellular domains provides an important mechanism to spatially and temporally introduce new proteins in polarized cells. Neurons make use of this localized protein synthesis during initial growth, regeneration and functional maintenance of their axons. Although ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2018-04-13 00:00:00
abstract：:Ligand-dependent transcriptional regulation by nuclear receptors is believed to be mediated by intermediary factors (TIFs) acting on remodelling of the chromatin structure and/or the activity of the transcriptional machinery. The putative transcriptional mediator TIF1alpha is a nuclear protein kinase that has been ide...
journal_title：Journal of cell science
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doi：
更新日期：1999-06-01 00:00:00
abstract：:The pericentriolar recycling endosome (RE) may be an alternative compartment through which some beta2-adrenergic receptors (beta2ARs) recycle from early endosomes to the cell surface during prolonged exposure to agonist. For the transferrin receptor, CXCR2, and the M4-muscarinic acetylcholine receptor, trafficking thr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01168
更新日期：2004-07-01 00:00:00
abstract：:Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, ...
journal_title：Journal of cell science
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更新日期：1978-02-01 00:00:00
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更新日期：2012-02-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00137
更新日期：2002-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2014-12-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1982-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2018-12-19 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01302
更新日期：2004-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1993-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1994-02-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2006-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.059519
更新日期：2010-05-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2006-04-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1990-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1989-02-01 00:00:00
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更新日期：2004-11-15 00:00:00
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更新日期：1982-04-01 00:00:00
