Abstract：
:Endocytosis of chemokine receptors regulates signal transduction initiated by chemokines, but the molecular mechanisms underlying this process are not fully defined. In this work, we assessed the involvement of the motor protein nonmuscle myosin heavy chain IIA (MIIA) in the endocytosis of CXCR4 induced by SDF-1alpha (also known as CXCL12) in T lymphocytes. Overexpression of the C-terminal half of MIIA inhibited the ligand-induced endocytosis of CXCR4, but not that of transferrin receptor. Targeting MIIA either by silencing its expression with small interfering RNA (siRNA) or by blebbistatin treatment also inhibited endocytosis of CXCR4. Inhibition of endocytosis of CXCR4 by targeting endogenous MIIA resulted in an increased migration of T cells induced by SDF-1alpha, and in the inhibition of the HIV-1-Env antifusogenic activity of this chemokine. Coimmunoprecipitation and protein-protein binding studies demonstrated that MIIA interacts with both the cytoplasmic tail of CXCR4 and beta-arrestin. Moreover, SDF-1alpha promotes a rapid MIIA-beta-arrestin dissociation. Our data reveal a novel role for MIIA in CXCR4 endocytosis, which involves its dynamic association with beta-arrestin and highlights the role of endogenous MIIA as a regulator of CXCR4 internalization and, therefore, the onset of SDF-1alpha signaling.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Rey M,Valenzuela-Fernández A,Urzainqui A,Yáñez-Mó M,Pérez-Martínez M,Penela P,Mayor F Jr,Sánchez-Madrid Fdoi
10.1242/jcs.03415subject
Has Abstractpub_date
2007-03-15 00:00:00pages
1126-33issue
Pt 6eissn
0021-9533issn
1477-9137pii
jcs.03415journal_volume
120pub_type
杂志文章abstract：:Arf GTPases regulate both the morphological and protein sorting events that are essential for membrane trafficking. Guanine nucleotide exchange factors (GEFs) specific for Arf proteins determine when and where Arf GTPases will be activated in cells. The yeast Gea2p Arf GEF is a member of an evolutionarily conserved fa...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00896
更新日期：2004-02-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthesis (hydroxyurea and 1-beta-D-arabinofuranosylcytosine) after ultraviolet irradiation we have quantitated an early step in the repair process - the frequency of single-strand DNA breaks, products of enzymic incision--in...
journal_title：Journal of cell science
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更新日期：1983-03-01 00:00:00
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doi：10.1242/jcs.075770
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：10.1242/jcs.124586
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.187112
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pub_type： 杂志文章
doi：10.1242/jcs.005751
更新日期：2007-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.007948
更新日期：2007-09-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1996-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.054536
更新日期：2009-12-15 00:00:00
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更新日期：2012-08-15 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1995-04-01 00:00:00
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doi：
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更新日期：1989-02-01 00:00:00
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更新日期：2009-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
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更新日期：1993-12-01 00:00:00
