Abstract:
:The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) coordinately measuring mitotic induction and DNA content of bimE7 mutant cells; and (2) analyzing epistasis relationships between bimE7 and 16 different nim mutations. A combination of cytological and flow cytometric techniques was used to show that bimE7 cells at restrictive temperature (44 degrees C) undergo a normal, although somewhat slower cell cycle prior to mitotic arrest. Most bimE7 cells were fully reversible from restrictive temperature arrest, indicating that they are able to enter mitosis normally, and therefore require bimE function in order to finish mitosis. Furthermore, epistasis studies between bimE7 and mutations in cdc2 pathway components revealed that the induction of mitosis caused by inactivation of bimE requires functional p34cdc2 kinase, and that mitotic induction by bimE7 depends upon several other nim genes whose functions are not yet known. The involvement of bimE in S phase function and mitotic checkpoint control was suggested by three lines of evidence. First, at restrictive temperature the bimE7 mutation slowed the cell cycle by delaying the onset or execution of S phase. Second, at permissive temperature (30 degrees C) the bimE7 mutation conferred enhanced sensitivity to the DNA synthesis inhibitor hydroxyurea. Finally, the checkpoint linking M phase to the completion of S phase was abolished when bimE7 was combined with two nim mutations that cause arrest in G1 or S phase. A model for bimE function based on these findings is presented.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
James SW,Mirabito PM,Scacheri PC,Morris NRsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
3485-99eissn
0021-9533issn
1477-9137journal_volume
108 ( Pt 11)pub_type
杂志文章abstract::The apical region of the Drosophila testis contains a niche with two stem cell populations: germline stem cells (GSCs) and cyst progenitor cells (CPCs). Asymmetrical division of these stem cells leads to gonioblast daughters (which undergo further mitoses) and cyst cell daughters (which withdraw from the cell cycle an...
journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:1994-12-01 00:00:00
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更新日期:2003-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-02-28 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02757
更新日期:2006-02-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2001-02-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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pub_type: 杂志文章,评审
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更新日期:2004-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::In the presence of sialic acid donors Trypanosoma cruzi acquires up to 10(7) sialic acid residues on its surface, in a reaction catalyzed by its unique trans-sialidase. Most of these sialic acid residues are incorporated into mucin-like glycoproteins. To further understand the biological role of parasite sialylation, ...
journal_title:Journal of cell science
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更新日期:2000-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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