Apical beta 1 integrin in polarized MDCK cells mediates tubulocyst formation in response to type I collagen overlay.

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:The secreted Frizzled-related proteins (SFRPs) are a family of soluble proteins that are structurally related to Frizzled (Fz) proteins, the serpentine receptors that mediate the extensively used cell-cell communication pathway involving Wnt signalling. Because of their homology with the Wnt-binding domain on the Fz r...

journal_title：Journal of cell science

authors： Bovolenta P,Esteve P,Ruiz JM,Cisneros E,Lopez-Rios J

更新日期：2008-03-15 00:00:00

abstract：:Insect flight muscle has a large component (Tn-H) in the tropomyosin-troponin complex that is not present in vertebrate striated muscle thin filaments. Tn-H is shown by gold/Fab labelling to be present at regular intervals in insect flight muscle thin filaments. The Fab fragment of a monoclonal antibody to Tn-H was co...

journal_title：Journal of cell science

authors： Newman R,Butcher GW,Bullard B,Leonard KR

更新日期：1992-03-01 00:00:00

abstract：:Interferon regulatory factor 6 (Irf6) regulates keratinocyte proliferation and differentiation. In this study, we tested the hypothesis that Irf6 regulates cellular migration and adhesion. Irf6-deficient embryos at 10.5 days post-conception failed to close their wound compared with wild-type embryos. In vitro, Irf6-de...

journal_title：Journal of cell science

authors： Biggs LC,Naridze RL,DeMali KA,Lusche DF,Kuhl S,Soll DR,Schutte BC,Dunnwald M

更新日期：2014-07-01 00:00:00

abstract：:Centriolar satellites are membrane-less structures that localize and move around the centrosome and cilium complex in a microtubule-dependent manner. They play important roles in centrosome- and cilium-related processes, including protein trafficking to the centrosome and cilium complex, and ciliogenesis, and they are...

journal_title：Journal of cell science

authors： Conkar D,Culfa E,Odabasi E,Rauniyar N,Yates JR 3rd,Firat-Karalar EN

更新日期：2017-04-15 00:00:00

abstract：:ADP-ribosylation factors (Arfs) and ADP-ribosylation factor-like proteins (Arls) are highly conserved small GTPases that function as main regulators of vesicular trafficking and cytoskeletal reorganization. Arl1, the first identified member of the large Arl family, is an important regulator of Golgi complex structure ...

journal_title：Journal of cell science

authors： Yu CJ,Lee FJ

更新日期：2017-05-15 00:00:00

abstract：:Human procathepsin D carries two N-linked glycosylation sites at asparagine residues 70 and 199, widely separated on the surface of the folded protein. We created monoglycosylated procathepsin D molecules by site-directed mutagenesis in vitro of the individual glycosylation sites. With only two exceptions, all 12 muta...

journal_title：Journal of cell science

authors： Fortenberry SC,Schorey JS,Chirgwin JM

更新日期：1995-05-01 00:00:00

abstract：:We have evaluated a series of laminarin sulphates with different degrees of sulphation (0.3-2.3) as antagonists of basic fibroblast growth factor (bFGF) and as inhibitors of the bFGF-dependent endothelial cell line FBHE. Inhibition of binding of bFGF by the laminarin sulphates increased with increasing degree of sulph...

journal_title：Journal of cell science

authors： Hoffman R,Paper DH,Donaldson J,Alban S,Franz G

更新日期：1995-11-01 00:00:00

abstract：:Insect ovarian Sf9 cells extend processes with complex morphologies when infected with a recombinant baculovirus encoding the catalytic subunit of protein kinase A. Within the shafts of the processes are abundant microtubules, which, in contrast to those in Sf9 cells expressing the microtubule-associated protein tau, ...

journal_title：Journal of cell science

authors： Cheley S,Kosik KS,Paskevich P,Bakalis S,Bayley H

更新日期：1992-08-01 00:00:00

abstract：:The conjugation junction of Tetrahymena is the specialized site where plasma membrane fusion occurs between two cells of complementary mating types. The junction is constructed through a series of cooperative interactions and morphogenetic steps. A contact-mediated interaction between free-swimming, sexually mature an...

journal_title：Journal of cell science

authors： Wolfe J

更新日期：1985-02-01 00:00:00

abstract：:Annexins are cytosolic phospholipid-binding proteins that can be found on the outer leaflet of the plasma membrane. The extracellular functions of annexin include modulating fibrinolysis activity and cell migration. Despite having well-described extracellular functions, the mechanism of annexin transport from the cyto...

journal_title：Journal of cell science

authors： Stewart SE,Ashkenazi A,Williamson A,Rubinsztein DC,Moreau K

更新日期：2018-07-19 00:00:00

abstract：:Microinjected GTP gamma S revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII dependent export of ts-O45-G from the ER is blocked in cells injected with recombinant p...

journal_title：Journal of cell science

authors： Pepperkok R,Lowe M,Burke B,Kreis TE

更新日期：1998-07-01 00:00:00

abstract：:Cystogenesis is a morphological consequence of numerous genetic diseases of the epithelium. In the kidney, the pathogenic mechanisms underlying the program of altered cell and tubule morphology are obscured by secondary effects of cyst expansion. Here, we developed a new 3D tubuloid system to isolate the rapid changes...

journal_title：Journal of cell science

authors： Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

更新日期：2020-07-16 00:00:00

abstract：:In vitro experimental evidences suggest that the proteolytic degradation of the extracellular matrix (ECM) by activation of the urokinase-type plasminogen activator (uPA)/plasmin system may affect growth factor activity and bioavailability. However, no direct in vivo observations were available to support this hypothe...

journal_title：Journal of cell science

authors： Ribatti D,Leali D,Vacca A,Giuliani R,Gualandris A,Roncali L,Nolli ML,Presta M

更新日期：1999-12-01 00:00:00

abstract：:Aggregation capacity of chicken embryo chondrocytes decreases when transformed by Rous sarcoma viruses. Cell-to-cell aggregation capacity of chondrocytes infected with a T class temperature-sensitive mutant (tsNY68) (with the temperature-sensitive lesion at the src gene) of Rous sarcoma virus is dependent upon the tem...

journal_title：Journal of cell science

authors： Tanaka A,Kaji A

更新日期：1980-06-01 00:00:00

abstract：:Pieces of small intestine from newborn, I- and 6-day-old piglets were incubated in vitro and ligated segments were incubated in vivo with polycationic ferritin (PCF) to determine the distribution and possible role in protein uptake of anionic sites on membranes at the luminal surfaces of the epithelial cells. Most PCF...

journal_title：Journal of cell science

authors： Chase CC,Munn EA

更新日期：1980-12-01 00:00:00

abstract：:The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...

journal_title：Journal of cell science

authors： Pérez-Castro AJ,Freire R

更新日期：2012-03-01 00:00:00

abstract：:Cell surface glycans are believed to play a role in tumour invasion and metastasis. Yet, we have previously shown that the inhibitors of N-linked glycan processing swainsonine (SW) and 1-deoxynojirimycin (dNM) did not prevent invasion of chick heart fragments by MO4 murine fibrosarcoma cells in organ culture. We now p...

journal_title：Journal of cell science

authors： Bruyneel EA,De Mets M,Dragonetti CH,Hooghe RJ,Di Virgilio S,Mareel MM

更新日期：1990-02-01 00:00:00

abstract：:This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous 'balloon' with two boundary structures. One of these - the collar -...

journal_title：Journal of cell science

authors： Lacomble S,Vaughan S,Gadelha C,Morphew MK,Shaw MK,McIntosh JR,Gull K

更新日期：2009-04-15 00:00:00

abstract：:Efficient trafficking of the cystic fibrosis transmembrane conductance regulator (CFTR) to and from the cell surface is essential for maintaining channel density at the plasma membrane (PM) and ensuring proper physiological activity. The most common mutation, F508del, exhibits reduced surface expression and impaired f...

journal_title：Journal of cell science

authors： Holleran JP,Zeng J,Frizzell RA,Watkins SC

更新日期：2013-06-15 00:00:00

abstract：:Cells constantly adapt to various environmental changes and stresses. The way in which nutrient and stress levels in a cell feed back to control metabolism and growth are, unsurprisingly, extremely complex, as responding with great sensitivity and speed to the 'feast or famine, slack or stress' status of its environme...

journal_title：Journal of cell science

authors： Dokudovskaya S,Rout MP

更新日期：2015-06-15 00:00:00

abstract：:The structure of a late endosomal compartment, which contains the bulk of the cation-independent mannose 6-phosphate receptor (MPR) in NRK cells, is documented using immunocytochemistry and cryo-sections, as well as conventional Epon sections. This compartment, which we refer to as the prelysosomal compartment (PLC), ...

journal_title：Journal of cell science

authors： Griffiths G,Matteoni R,Back R,Hoflack B

更新日期：1990-03-01 00:00:00

abstract：:A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin...

journal_title：Journal of cell science

authors： Tang XJ,Hepler PK,Scordilis SP

更新日期：1989-04-01 00:00:00

abstract：:The conformation and biological activities of fibronectin (FN) and vitronectin (VN) coated on different plastic surfaces were investigated using cell adhesion and a panel of domain-specific monoclonal antibodies (mAbs). The adhesion of BHK fibroblasts was markedly better on FN coated on hydrophilic tissue culture poly...

journal_title：Journal of cell science

authors： Underwood PA,Steele JG,Dalton BA

更新日期：1993-03-01 00:00:00

abstract：:Deubiquitylating (or deubiquitinating) enzymes (DUBs) are proteases that reverse protein ubiquitylation and therefore modulate the outcome of this post-translational modification. DUBs regulate a variety of intracellular processes, including protein turnover, signalling pathways and the DNA damage response. They have ...

journal_title：Journal of cell science

authors： Leznicki P,Kulathu Y

更新日期：2017-06-15 00:00:00

abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...

journal_title：Journal of cell science

authors： Sauer M

更新日期：2013-08-15 00:00:00

abstract：:Distributions of ErbB receptors on membranes of SKBR3 breast cancer cells were mapped by immunoelectron microscopy. The most abundant receptor, ErbB2, is phosphorylated, clustered and active. Kinase inhibitors ablate ErbB2 phosphorylation without dispersing clusters. Modest co-clustering of ErbB2 and EGFR, even after ...

journal_title：Journal of cell science

authors： Yang S,Raymond-Stintz MA,Ying W,Zhang J,Lidke DS,Steinberg SL,Williams L,Oliver JM,Wilson BS

更新日期：2007-08-15 00:00:00

abstract：:In the presence of sialic acid donors Trypanosoma cruzi acquires up to 10(7) sialic acid residues on its surface, in a reaction catalyzed by its unique trans-sialidase. Most of these sialic acid residues are incorporated into mucin-like glycoproteins. To further understand the biological role of parasite sialylation, ...

journal_title：Journal of cell science

authors： Pereira-Chioccola VL,Acosta-Serrano A,Correia de Almeida I,Ferguson MA,Souto-Padron T,Rodrigues MM,Travassos LR,Schenkman S

更新日期：2000-04-01 00:00:00

abstract：:Little is known of the molecular basis for the diversity of microtubule structure and function found within the eukaryotic flagellum. Antibodies that discriminate between tyrosinated alpha tubulin and post-translationally detyrosinated alpha tubulin were used to localize these complementary tubulin isoforms in flagell...

journal_title：Journal of cell science

authors： Johnson KA

更新日期：1998-02-01 00:00:00

abstract：:Trypsin digestion of demembranated fowl spermatozoa caused a longitudinal splitting of the distal part of the axoneme. The resulting strands, consisting of groups of doublet microtubules, formed left-handed helices. On the evidence of electron micrographs, the digestion had caused the loss of dynein arms from the oute...

journal_title：Journal of cell science

authors： Woolley DM,Brammall A

更新日期：1987-10-01 00:00:00