Abstract:
:Cell surface glycans are believed to play a role in tumour invasion and metastasis. Yet, we have previously shown that the inhibitors of N-linked glycan processing swainsonine (SW) and 1-deoxynojirimycin (dNM) did not prevent invasion of chick heart fragments by MO4 murine fibrosarcoma cells in organ culture. We now present biochemical evidence that these and other inhibitors of processing were indeed effective in remodeling glycans, including those expressed at the cell surface. After metabolic labeling with tritiated mannose or fucose, glycosylpeptides were obtained by Pronase treatment of material released from intact cells by trypsin. Glycosylpeptides were separated by Biogel P-10 chromatography. With all drugs tested, there was a shift towards lower molecular weight of the glycan chains. There were, however, major quantitative differences between the different drugs and also, for monensin (MON; 0.1 microgram ml-1), between fucose-labeled and mannose-labeled chains. The shift in apparent molecular weight affected mainly fucose-labeled peptides after treatment of MO4 cells with SW (0.4 microgram ml-1). The shift induced by dNM (10 mM) + SW (0.4 microgram ml-1) in both fucosylated and mannosylated chains was much larger than that induced by SW given alone. 1-Deoxymannojirimycin (dMM; 1 mM) had major effects on both mannose and fucose-labeled structures and so did N-methyl-1-deoxynojirimycin (MdNM; 2 mM) and castanospermine (CS; 100 micrograms ml-1). With the latter drugs, incorporation of fucose in complex-type glycosylpeptides was dramatically reduced. The effect of SW on fucose-labeled glycosylpeptides of embryonic chick heart was similar to that observed on MO4 cells.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Bruyneel EA,De Mets M,Dragonetti CH,Hooghe RJ,Di Virgilio S,Mareel MMsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
279-86eissn
0021-9533issn
1477-9137journal_volume
95 ( Pt 2)pub_type
杂志文章abstract::Blocking expression of EhCaBP1, a calmodulin-like, four EF-hand protein from the protozoan parasite Entamoeba histolytica, resulted in inhibition of cellular proliferation. In this paper we report that EhCaBP1 is involved in dynamic changes of the actin cytoskeleton. Both endocytosis and phagocytosis were severely imp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01198
更新日期:2004-07-15 00:00:00
abstract::Syntaxins are thought to participate in the specific interactions between vesicles and acceptor membranes in intracellular protein trafficking. VAM3 of Saccharomyces cerevisiae encodes a 33 kDa protein (Vam3p) with a hydrophobic transmembrane segment at its C terminus. Vam3p has structural similarities to syntaxins of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::In order to study the dynamic behavior of intermediate filament networks in living cells, we have prepared constructs fusing green fluorescent protein to intermediate filament proteins. Vimentin fused to green fluorescent protein labeled the endogenous intermediate filament network. We generated stable SW13 and NIH3T3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Patients with myotonic dystrophy type 1 exhibit a diversity of symptoms that affect many different organs. Among these are cognitive dysfunctions, the origin of which has remained elusive, partly because of the difficulty in accessing neural cells. Here, we have taken advantage of pluripotent stem cell lines derived f...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.116285
更新日期:2013-04-15 00:00:00
abstract::The events responsible for repair of DNA interstrand cross-links in mammalian cells, the proteins involved and their interactions with each other are poorly understood. The present study demonstrates that the structural protein nonerythroid alpha spectrin (alphaSpIISigma*), present in normal human cell nuclei, plays a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00294
更新日期:2003-03-01 00:00:00
abstract::Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Purified nuclei retaining a high degree of ultrastructural integrity were isolated by conventional centrifugation techniques. The cytoplasmic surface of these nuclei was iodinated using lactoperoxidase immobilized onto giant Sepharose beads; thus the outer nuclear membrane and the cytoplasmic surface of nuclear pore c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Notch signaling is pivotal for the morphogenesis and homeostasis of many tissues. We found that aberrant Notch activation in mouse neural-crest-derived periocular mesenchymal cells (POMCs), which contribute to the formation of corneal and eyelid stroma, results in blepharophimosis. Compound transgenic mice overexpress...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.085001
更新日期:2011-08-01 00:00:00
abstract::Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing mela...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216226
更新日期:2018-09-20 00:00:00
abstract::The vacuolar H(+)-ATPase (V-ATPase) establishes pH gradients along secretory and endocytic pathways. Progressive acidification is essential for proteolytic processing of prohormones and aggregation of soluble content proteins. The V-ATPase V(0) subunit is thought to have a separate role in budding and fusion events. P...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.034298
更新日期:2009-10-01 00:00:00
abstract::Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA. Using a double-labelling procedure we have compared, at different salt concentrations, t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Human procathepsin D carries two N-linked glycosylation sites at asparagine residues 70 and 199, widely separated on the surface of the folded protein. We created monoglycosylated procathepsin D molecules by site-directed mutagenesis in vitro of the individual glycosylation sites. With only two exceptions, all 12 muta...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic human disease, with around 12.5 million people affected worldwide. ADPKD results from mutations in either PKD1 or PKD2, which encode the atypical G-protein coupled receptor polycystin-1 (PC1) and the transient receptor potential channel ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.211342
更新日期:2018-06-06 00:00:00
abstract::Mammalian, or mechanistic, target of rapamycin complex 2 (mTORC2) regulates a variety of vital cellular processes, and its aberrant functioning is often associated with various diseases. Rictor is a peculiar and distinguishing mTORC2 component playing a pivotal role in controlling its assembly and activity. Among exta...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.236505
更新日期:2019-11-14 00:00:00
abstract::The tissue concentration of an endogenous beta-galactoside-specific lectin is developmentally regulated in rabbit tissues during foetal and neonatal development. Immunoassay data and localization studies both indicate that the lectin is particularly associated with late embryonic and early postnatal development. No le...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::This study describes the functional characterization of two cell lines derived from the proximal convoluted (PKSV-PCT cells) and proximal straight (PKSV-PR) tubules microdissected out from kidneys of transgenic mice harboring the simian virus 40 (SV40) large T and small t antigens placed under the control of the rat L...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Galectin-3 is a member of a closely related family of beta-galactoside-binding soluble proteins found in many vertebrate epithelial and myeloid cell types. The developmentally regulated presence of galectin-3 in tissues, for example kidney, and an affinity for many cell-surface and matrix glycoproteins indicate its im...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The interaction of promastigotes of the protozoan parasite Leishmania tropica with mouse peritoneal macrophages in vitro was studied by scanning electron microscopy. Motile promastigotes attached to host cells by their flagellar tips to which the macrophages responded by producing rather closely fitting lamellar sheat...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::We have isolated mini-titin from the nematodes Ascaris lumbricoides and Caenorhabditis elegans under native conditions using a modification in the procedure to prepare this protein from insect muscle. The proteins have an apparent molecular weight of 600,000 and appear in oriented specimens as flexible thin rods with ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::In human red blood cells, protein 4.1 (4.1R) stabilizes the spectrin-actin network and anchors it to the plasma membrane. To contribute to the characterization of functional roles of 4.1R in nonerythroid cells, we analysed the effect of ectopic expression of 4.1R isoforms on interphase microtubules in fibroblastic cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01544
更新日期:2004-12-01 00:00:00
abstract::Recent biochemical and molecular approaches have begun to establish the protein interactions that lead to desmosome assembly. To determine whether these associations occur in native desmosomes we have performed ultrastructural localisation of specific domains of the major desmosomal components and have used the result...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Uptake of cadmium into cultured cells and its effects on cell growth and DNA synthesis are measured over a range of Cd concentrations of seven orders of magnitude. Cd uptake is found to be proportional to the external Cd concentration and to incubation time over a very broad range of concentrations. At least 200 mmol ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::The adaptor protein SETA/CIN85/Ruk is involved in regulating diverse signal transduction pathways, including the internalization of tyrosine kinase receptors via the Cbl ubiquitin ligases, and attenuating PI3K activity by interaction with its regulatory subunit. Here we present evidence for a new aspect of SETA functi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00522
更新日期:2003-07-15 00:00:00
abstract::In mammals, fertilization initiates Ca2+ oscillations in metaphase II oocytes, which are required for the activation of embryo development. Germinal vesicle (GV) oocytes also display Ca2+ oscillations, although these unfold spontaneously in the absence of any known agonist(s) and their function remains unclear. We fou...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.225441
更新日期:2019-02-12 00:00:00
abstract::During spermatogenesis, the first morphological indication of spermatogonia differentiation is incomplete cytokinesis, followed by the assembly of stable intercellular cytoplasmic communications. This distinctive feature of differentiating male germ cells has been highly conserved during evolution, suggesting that reg...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00163
更新日期:2002-12-15 00:00:00
abstract::Mob1 is a component of both the mitotic exit network and Hippo pathway, being required for cytokinesis, control of cell proliferation and apoptosis. Cell division accuracy is crucial in maintaining cell ploidy and genomic stability and relies on the correct establishment of the cell division axis, which is under the c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.096610
更新日期:2012-01-15 00:00:00
abstract::The structural events in the stigma and transmitting tissue of Petunia hybrida pistils that accompany compatible and incompatible intraspecific pollinations have been investigated in detail, together with the changes in reserve levels that also take place at this time. Many of these phenomena may be explained in terms...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::RAS isoforms have been proposed to exhibit differing biological outputs due to differences in their relative occupancy of cellular organelles and signalling microdomains. The membrane binding and targeting motifs of RAS are encoded by the C-terminal hypervariable region (HVR), and the precise localisation depends upon...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.020107
更新日期:2008-02-15 00:00:00
abstract::MACF (microtubule actin cross-linking factor) is a large, 608-kDa protein that can associate with both actin microfilaments and microtubules (MTs). Structurally, MACF can be divided into 3 domains: an N-terminal domain that contains both a calponin type actin-binding domain and a plakin domain; a rod domain that is co...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00