Abstract:
:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assays, although their action does not seem to depend upon their anti-protease capacity. Contrary to expectation, leucocyte movement over surfaces and through both rigid and deformable three-dimensional matrices (micropore filters and collagen gels) is unaffected by antiproteases. This renders the idea that forward movement depends upon the proteolytic cleavage of redundant adhesion sites less probable. Various non-physiological antiproteases (chloromethyl ketones, soya bean trypsin inhibitor, and epsilon-aminocaproic acid are also ineffective in reducing locomotion, although they do cause minor changes in adhesiveness.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Forrester JV,Lackie JM,Brown AFsubject
Has Abstractpub_date
1983-01-01 00:00:00pages
213-30eissn
0021-9533issn
1477-9137journal_volume
59pub_type
杂志文章abstract::The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. Nuclear proteins that are localized in discrete domains within the nucleus include the leukemia-associated acute myelogenous leukemia (AML) an...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-07-01 00:00:00
abstract::Epitectin, the mucin-like glycoprotein defined by the monoclonal antibodies CA1, CA2 and CA3, has been examined by electron microscopy to determine its shape and size. It appears to be a single extended strand with a mean length of about 270 nm. The antibodies CA1 and CA2 appear to bind preferentially to a terminal si...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.213496
更新日期:2019-07-30 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.180190
更新日期:2016-04-15 00:00:00
abstract::CENP-C, one of the few known intrinsic proteins of the human centromere, is thought to play structural as well as regulatory roles crucial to proper chromosome segregation and mitotic progression. To further define the functions of CENP-C throughout the cell cycle we have used the yeast interaction trap to identify pr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-30 00:00:00
abstract::hnRNP A1 (34 kDa) is an RNA binding protein consisting of two tandemly arranged RNA binding domains C-terminally linked to a glycine-rich auxiliary domain (2 x RBD-Gly). A1 belongs to the set of polypeptides that bind nascent hnRNA in the nucleus to form the so called hnRNP complexes. These complexes seem to be involv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03455
更新日期:2007-06-01 00:00:00
abstract::The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results suppo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.036038
更新日期:2008-12-15 00:00:00
abstract::Evidence from various systems suggests that either asters or the midzone of the mitotic spindle are the predominant determinants of cleavage plane position. Disrupting spindle midzone formation in the one-cell Caenorhabditis elegans embryo, such as by using mutants of the centralspindlin component ZEN-4, prevents comp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03447
更新日期:2007-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03221
更新日期:2006-12-15 00:00:00
abstract::Alix is a cytosolic protein in mammalian cells that was originally identified on the basis of its association with pro-apoptotic signaling. More recent evidence has established that Alix has a hand in regulating other cellular mechanisms, including endocytic membrane trafficking and cell adhesion. Although Alix appear...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03072
更新日期:2006-08-01 00:00:00
abstract::Many of the cilia- and flagella-specific integral membrane proteins identified to date function to sense the extracellular milieu, and there is considerable interest in defining pathways for targeting such proteins to these sensory organelles. The flagellar glucose transporter of Leishmania mexicana, LmxGT1, is target...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.103028
更新日期:2012-07-15 00:00:00
abstract::Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00302
更新日期:2003-04-01 00:00:00
abstract::Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.172072
更新日期:2015-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::TANGLED (TAN) is the founding member of a family of plant-specific proteins required for correct orientation of the division plane. Arabidopsis thaliana TAN is localized before prophase until the end of cytokinesis at the cortical division site (CDS), where it appears to help guide the cytokinetic apparatus towards th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.073676
更新日期:2011-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2016-10-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::A glass neodymium laser was modified to make it possible to produce small lesions of 1-2 micron size with a quantitatively known amount of energy. The 1-06-micron radiation of this laser is sufficiently absorbed by water to work without the additions of dyes. Ciliary arrest in Phragmatopoma gills was produced by an am...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson’s disease, but the precise physiological function of the protein remains ill-defined. Recently, our group proposed a model in which LRRK2 kinase activity is part of an EndoA phosphorylation cycle that facilitates efficient vesicle formatio...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-04-15 00:00:00
abstract::As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its sy...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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更新日期:2006-01-01 00:00:00