Rab11 regulates the recycling and lysosome targeting of beta2-adrenergic receptors.

Abstract:

:The pericentriolar recycling endosome (RE) may be an alternative compartment through which some beta2-adrenergic receptors (beta2ARs) recycle from early endosomes to the cell surface during prolonged exposure to agonist. For the transferrin receptor, CXCR2, and the M4-muscarinic acetylcholine receptor, trafficking through the RE and receptor recycling is regulated by the small GTPase rab11. The precise role of the RE and rab11 in regulating the cellular trafficking of the beta2AR is not understood. We therefore monitored trafficking of beta2ARs in HEK293 cells following the modulation of rab11 activity. Expression of a rab11 mutant deficient in GTP binding (as a fusion between enhanced green fluorescent protein (EGFP) and the rab11S25N mutant) significantly slowed receptor recycling to the cell surface from dispersed transferrin-positive peripheral vesicles following a brief exposure to agonist. The agonist was applied at a time when receptors have undergone only one or two rounds of endocytosis and recycling. In cells overexpressing wild-type rab11, beta2ARs localized to a rab11-positive compartment and the rate of beta2AR recycling to the cell surface was reduced, but only after prolonged exposure to agonist and multiple rounds of receptor endocytosis and recycling. This effect was associated with impaired beta2AR trafficking to lysosomes and receptor proteolysis, whereas the sorting of low-density lipoprotein from transferrin-positive vesicles to late endosomes and lysosomes was not affected. These data highlight a pivotal role for rab11 in regulating the traffic of a G protein-coupled receptor at the level of the RE, where modulation of rab11 activity dictates the balance between receptor recycling and downregulation during prolonged exposure to agonist.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Moore RH,Millman EE,Alpizar-Foster E,Dai W,Knoll BJ

doi

10.1242/jcs.01168

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

3107-17

issue

Pt 15

eissn

0021-9533

issn

1477-9137

pii

jcs.01168

journal_volume

117

pub_type

杂志文章
  • The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content.

    abstract::Little is known of the molecular basis for the diversity of microtubule structure and function found within the eukaryotic flagellum. Antibodies that discriminate between tyrosinated alpha tubulin and post-translationally detyrosinated alpha tubulin were used to localize these complementary tubulin isoforms in flagell...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Johnson KA

    更新日期:1998-02-01 00:00:00

  • Down-regulation of actin genes precedes microfilament network disruption and actin cleavage during p53-mediated apoptosis.

    abstract::Inactivation of Simian Virus 40 large T antigen, in cells immortalized with conditional mutants, leads to activation of p53 and apoptosis. We used the mRNA differential display method to identify genes differentially expressed during this process. We found that steady-state levels of mRNA for cytoplasmic actins decrea...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Guénal I,Risler Y,Mignotte B

    更新日期:1997-02-01 00:00:00

  • Galectin-3 expression and effects on cyst enlargement and tubulogenesis in kidney epithelial MDCK cells cultured in three-dimensional matrices in vitro.

    abstract::Galectin-3 is a member of a closely related family of beta-galactoside-binding soluble proteins found in many vertebrate epithelial and myeloid cell types. The developmentally regulated presence of galectin-3 in tissues, for example kidney, and an affinity for many cell-surface and matrix glycoproteins indicate its im...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Bao Q,Hughes RC

    更新日期:1995-08-01 00:00:00

  • A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1.

    abstract::ARF-like proteins (ARLs) are distinct group of members of the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00123

    authors: Lin CY,Li CC,Huang PH,Lee FJ

    更新日期:2002-12-01 00:00:00

  • Inhibition of apical but not basolateral endocytosis of ricin and folate in Caco-2 cells by cytochalasin D.

    abstract::Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Jackman MR,Shurety W,Ellis JA,Luzio JP

    更新日期:1994-09-01 00:00:00

  • In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent.

    abstract::The protozoan parasite Giardia lamblia has the unusual morphology of bearing two equal-sized nuclei. This organism probably represents the earliest diverging lineage of eukaryotes, suggesting that its biological tactics may be transitional. To begin to understand the role played by the two equal-sized nuclei in this o...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Kabnick KS,Peattie DA

    更新日期:1990-03-01 00:00:00

  • Syndecan-4 and integrins: combinatorial signaling in cell adhesion.

    abstract::It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...

    journal_title:Journal of cell science

    pub_type: 评论,杂志文章,评审

    doi:

    authors: Couchman JR,Woods A

    更新日期:1999-10-01 00:00:00

  • Hormone-induced meiotic maturation in Xenopus oocytes occurs independently of p70s6k activation and is associated with enhanced initiation factor (eIF)-4F phosphorylation and complex formation.

    abstract::Hormone-induced meiotic maturation of the Xenopus oocyte is regulated by complex changes in protein phosphorylation. It is accompanied by a stimulation in the rate of translation, manifest at the level of polypeptide chain initiation. At laser times in the maturation process, this reflects an increased ability for mRN...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Morley SJ,Pain VM

    更新日期:1995-04-01 00:00:00

  • Bacteroides fragilis toxin stimulates intestinal epithelial cell shedding and gamma-secretase-dependent E-cadherin cleavage.

    abstract::Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03455

    authors: Wu S,Rhee KJ,Zhang M,Franco A,Sears CL

    更新日期:2007-06-01 00:00:00

  • The heparin-binding domain of HB-EGF mediates localization to sites of cell-cell contact and prevents HB-EGF proteolytic release.

    abstract::Heparin-binding EGF-like growth factor (HB-EGF) is a ligand for EGF receptor (EGFR) and possesses the ability to signal in juxtacrine, autocrine and/or paracrine mode, with these alternatives being governed by the degree of proteolytic release of the ligand. Although the spatial range of diffusion of released HB-EGF i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.058321

    authors: Prince RN,Schreiter ER,Zou P,Wiley HS,Ting AY,Lee RT,Lauffenburger DA

    更新日期:2010-07-01 00:00:00

  • The expression of an Ets1 transcription factor lacking its activation domain decreases uPA proteolytic activity and cell motility, and impairs normal tubulogenesis and cancerous scattering in mammary epithelial cells.

    abstract::Cell migration and invasion play a crucial role during normal and pathological development. The expression of several members of the Ets family of transcription factors has been shown to correlate with the occurrence of these processes. In the present study, we investigated the effect of the expression of Ets1-DB, the...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Delannoy-Courdent A,Mattot V,Fafeur V,Fauquette W,Pollet I,Calmels T,Vercamer C,Boilly B,Vandenbunder B,Desbiens X

    更新日期:1998-06-01 00:00:00

  • A unique role for clathrin light chain A in cell spreading and migration.

    abstract::Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely respons...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.224030

    authors: Tsygankova OM,Keen JH

    更新日期:2019-05-15 00:00:00

  • Positive charges on the translocating polypeptide chain arrest movement through the translocon.

    abstract::Polypeptide chains synthesized by membrane-bound ribosomes are translocated through, and integrated into, the endoplasmic reticulum (ER) membrane by means of the protein translocation channel, the translocon. Positive charges on the nascent chain determine the orientation of the hydrophobic segment as it is inserted i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.086850

    authors: Fujita H,Yamagishi M,Kida Y,Sakaguchi M

    更新日期:2011-12-15 00:00:00

  • Phosphorylation of a major GPI-anchored surface protein of Trypanosoma brucei during transport to the plasma membrane.

    abstract::The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Bütikofer P,Vassella E,Ruepp S,Boschung M,Civenni G,Seebeck T,Hemphill A,Mookherjee N,Pearson TW,Roditi I

    更新日期:1999-06-01 00:00:00

  • Molecular cloning of a human homologue of Drosophila heterochromatin protein HP1 using anti-centromere autoantibodies with anti-chromo specificity.

    abstract::We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10-15% of sera from patients with anti-centromere...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Saunders WS,Chue C,Goebl M,Craig C,Clark RF,Powers JA,Eissenberg JC,Elgin SC,Rothfield NF,Earnshaw WC

    更新日期:1993-02-01 00:00:00

  • TOM1 is a PI5P effector involved in the regulation of endosomal maturation.

    abstract::Phosphoinositides represent a major class of lipids specifically involved in the organization of signaling cascades, maintenance of the identity of organelles and regulation of multiple intracellular trafficking steps. We previously reported that phosphatidylinositol 5-monophosphate (PI5P), produced by the Shigella fl...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.166314

    authors: Boal F,Mansour R,Gayral M,Saland E,Chicanne G,Xuereb JM,Marcellin M,Burlet-Schiltz O,Sansonetti PJ,Payrastre B,Tronchère H

    更新日期:2015-02-15 00:00:00

  • Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells.

    abstract::We have demonstrated the possibility of cross-talk between E-cadherin and alphav integrins in breast carcinoma cells. Using the function-blocking anti-alphav monoclonal antibody 17E6, applied to monolayer cultures of breast cancer lines, it was found that treatment of cells possessing detergent-insoluble (implying att...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: von Schlippe M,Marshall JF,Perry P,Stone M,Zhu AJ,Hart IR

    更新日期:2000-02-01 00:00:00

  • Mutation of an unusual mitochondrial targeting sequence of SODB2 produces multiple targeting fates in Toxoplasma gondii.

    abstract::Proteins destined for the mitochondria travel an intricate pathway through two membranes, each with its own receptors and channels. These proteins interact with receptors via N-terminal presequences that form amphipathic helices. Generally, these helices contain abundant positive charges on one face and hydrophobic re...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00750

    authors: Brydges SD,Carruthers VB

    更新日期:2003-11-15 00:00:00

  • Distinct metabolic states govern skeletal muscle stem cell fates during prenatal and postnatal myogenesis.

    abstract::During growth, homeostasis and regeneration, stem cells are exposed to different energy demands. Here, we characterise the metabolic pathways that mediate the commitment and differentiation of mouse skeletal muscle stem cells, and how their modulation can influence the cell state. We show that quiescent satellite stem...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.212977

    authors: Pala F,Di Girolamo D,Mella S,Yennek S,Chatre L,Ricchetti M,Tajbakhsh S

    更新日期:2018-07-27 00:00:00

  • Perturbation of mammalian cell division. III. The topography and kinetics of extrusion subdivision.

    abstract::If mitotic-arrested, cold-stored HeLa cells are incubated at 37 degrees C a proportion of the population divides by an aberrant process which we have called subdivision by extrusion. This process has been studied by time-lapse photography and shown to differ from normal cleavage in several respects. The cell surface b...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Mullinger AM,Johnson RT

    更新日期:1976-11-01 00:00:00

  • Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells.

    abstract::The main pathology of cystic fibrosis results from obstruction of ducts in several organs by mucous secretions. The cause of this obstruction remains unclear. We have examined expression of the cystic fibrosis transmembrane conductance regulator (CFTR) and of the major pancreatic mucin, MUC1, in primary pancreatic duc...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Chambers JA,Harris A

    更新日期:1993-06-01 00:00:00

  • p38 maintains E-cadherin expression by modulating TAK1-NF-kappa B during epithelial-to-mesenchymal transition.

    abstract::Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells is a pathological process that occurs during peritoneal dialysis. EMT leads to peritoneal fibrosis, ultrafiltration failure and eventually to the discontinuation of therapy. Signaling pathways involved in mesothelial EMT are thus of great inter...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.071647

    authors: Strippoli R,Benedicto I,Foronda M,Perez-Lozano ML,Sánchez-Perales S,López-Cabrera M,Del Pozo MÁ

    更新日期:2010-12-15 00:00:00

  • Transcriptional activation by the human Hsp70-associating protein Hap50.

    abstract::We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promo...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Niyaz Y,Zeiner M,Gehring U

    更新日期:2001-05-01 00:00:00

  • Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division.

    abstract::We have cloned and characterized a single copy C. reinhardtii gene containing an open reading frame of 333 nucleotides encoding a 12.7 kDa protein. The novel protein, DIP13, exhibits 60% identity with two mammalian proteins, human NA14 and an unnamed mouse protein. Homologous sequences are also present in several prot...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00337

    authors: Pfannenschmid F,Wimmer VC,Rios RM,Geimer S,Kröckel U,Leiherer A,Haller K,Nemcová Y,Mages W

    更新日期:2003-04-15 00:00:00

  • nimO, an Aspergillus gene related to budding yeast Dbf4, is required for DNA synthesis and mitotic checkpoint control.

    abstract::The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and re...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: James SW,Bullock KA,Gygax SE,Kraynack BA,Matura RA,MacLeod JA,McNeal KK,Prasauckas KA,Scacheri PC,Shenefiel HL,Tobin HM,Wade SD

    更新日期:1999-05-01 00:00:00

  • SUUR joins separate subsets of PcG, HP1 and B-type lamin targets in Drosophila.

    abstract::Drosophila melanogaster Suppressor of Under-Replication (SuUR) gene encodes a protein that modulates replicative properties of heterochromatin in endocycles of polytene cells. The SuUR mutation abolishes underreplication of intercalary heterochromatin and results in partial underreplication of pericentric heterochroma...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.006007

    authors: Pindyurin AV,Moorman C,de Wit E,Belyakin SN,Belyaeva ES,Christophides GK,Kafatos FC,van Steensel B,Zhimulev IF

    更新日期:2007-07-15 00:00:00

  • CCM1 regulates vascular-lumen organization by inducing endothelial polarity.

    abstract::Little is known about the molecular mechanisms that regulate the organization of vascular lumen. In this paper we show that lumen formation correlates with endothelial polarization. Adherens junctions (AJs) and VE-cadherin (VEC, encoded by CDH5) are required for endothelial apicobasal polarity in vitro and during embr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.059329

    authors: Lampugnani MG,Orsenigo F,Rudini N,Maddaluno L,Boulday G,Chapon F,Dejana E

    更新日期:2010-04-01 00:00:00

  • Involvement of aquaporin-4 in astroglial cell migration and glial scar formation.

    abstract::Aquaporin-4, the major water-selective channel in astroglia throughout the central nervous system, facilitates water movement into and out of the brain. Here, we identify a novel role for aquaporin-4 in astroglial cell migration, as occurs during glial scar formation. Astroglia cultured from the neocortex of aquaporin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02680

    authors: Saadoun S,Papadopoulos MC,Watanabe H,Yan D,Manley GT,Verkman AS

    更新日期:2005-12-15 00:00:00

  • Transdifferentiation of NRP-152 rat prostatic basal epithelial cells toward a luminal phenotype: regulation by glucocorticoid, insulin-like growth factor-I and transforming growth factor-beta.

    abstract::The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Danielpour D

    更新日期:1999-01-01 00:00:00

  • UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.

    abstract::In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.248815

    authors: Habicht J,Mooneyham A,Hoshino A,Shetty M,Zhang X,Emmings E,Yang Q,Coombes C,Gardner MK,Bazzaro M

    更新日期:2021-01-08 00:00:00