Abstract:
:Triton X-100-treated sperms were injected into non-nucleate egg fragments of Xenopus laevis to determine whether the structure of the injected sperm nucleus is affected by the cyclic activity of the cytoplasm. Swollen vesicular nuclei were very frequently observed when the sperms were injected and incubated during the 'rounding-up' phase of the recipient fragment, whereas no such structures were found when they were incubated during the 'relaxing' phase.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Sakai M,Shinagawa Asubject
Has Abstractpub_date
1983-09-01 00:00:00pages
69-76eissn
0021-9533issn
1477-9137journal_volume
63pub_type
杂志文章abstract::Cytoplasm of Paramecium caudatum syngen 3 in the period of sexual immaturity was effective in inducing immaturity when injected into mating-reactive cells of other syngens of P. caudatum. When immature cytoplasm of P. caudatum was injected into mature cells of P. multimicronucleatum, immaturity was also induced in the...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213116
更新日期:2018-08-10 00:00:00
abstract::Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.204321
更新日期:2018-08-09 00:00:00
abstract::The mammalian lens consists of an aged core of quiescent cells enveloped by a layer of synthetically active cells. Abundant gap junctions within and between these cell populations ensure that the lens functions as an electrical syncytium and facilitates the exchange of small molecules between surface and core cells. I...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00738
更新日期:2003-10-15 00:00:00
abstract::8-Hydroxyquinoline (HQ) chelates Mg2+ and Mn2+ and, secondarily, affects the activities of DNA and RNA polymerases. The in vivo effect of HQ has been estimated in Allium cepa L. meristems growing under new growth kinetics in the presence of this agent. HQ (at both 5 X 10(-5) M and 10(-4) M) depressed incorporation of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::The large GTPase dynamin is a mechanoenzyme that participates in the scission of nascent vesicles from the plasma membrane. Recently, dynamin has been demonstrated to associate with the Golgi apparatus in mammalian cells by morphological and biochemical methods. Additional studies using a well characterized, cell-free...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The small GTPase Arf4 and the Arf GTPase-activating protein (GAP) ASAP1 cooperatively sequester sensory receptor cargo into transport carriers targeted to primary cilia, but the input that drives Arf4 activation in this process remains unknown. Here, we show, by using frog retinas and recombinant human proteins, that ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.205492
更新日期:2017-12-01 00:00:00
abstract::Two isoforms of laminin were extracted from human placenta by neutral buffer containing EDTA, copurified through several steps and finally separated by Mono Q anion exchange chromatography. One variant consisted of disulphide-linked 340, 230 and 190 kDa subunits, which were identified by immunoblotting as Am, B1e and ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii, and found that it reacts with a single flagellar glycoprotein of about 230 kDa. Interestingly, immunofluorescence microscopy demonstra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Drosophila neural stem cells or neuroblasts undergo typical asymmetric cell division. An evolutionally conserved protein complex, comprising atypical protein kinase C (aPKC), Bazooka (Par-3) and Par-6, organizes cell polarity to direct these asymmetric divisions. Aurora-A (AurA) is a key molecule that links the divisi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.050955
更新日期:2009-09-15 00:00:00
abstract::A complex network of interactions between the stroma, the extracellular matrix and the epithelium drives mammary gland development and function. Two main assumptions in chemical carcinogenesis of the mammary gland have been that carcinogens induce neoplasia by causing mutations in the DNA of the epithelial cells and t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01000
更新日期:2004-03-15 00:00:00
abstract::It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::We have identified and characterized two proteins in rat brain that bind to the neuron-specific tropomyosin isoform, TMBr3. The two proteins were identified by blot overlay assay, in which the proteins immobilized on the membrane were probed by epitope-tagged TMBr3, followed by detection with anti-epitope antibody. We...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Present in organisms ranging from yeast to man, homologues of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme CDC34 have been shown to play important roles in the regulation of cell cycle progression and checkpoint function. Here we analyze the expression and intracellular localization of endogenous CDC34 du...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::To examine the internal structure of chromatin fibers, we have developed procedures for partial denaturation of small chromatin fragments (8-30 nucleosomes) from chicken erythrocytes. Electron micrographs of samples prepared under conditions that cause nucleosome dissociation show rods and loops projecting from short ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Activation and invasion of the vascular endothelium by Staphylococcus aureus is a major cause of sepsis and endocarditis. For endothelial cell invasion, S. aureus triggers actin polymerization through Cdc42, N-WASp (also known as WASL) and the Arp2/3 complex to assemble a phagocytic cup-like structure. Here, we show t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.186213
更新日期:2016-08-01 00:00:00
abstract::Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated in Wnt negative-feedback regulation. Here, we show that axin and conductin differ in their ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.159145
更新日期:2015-01-01 00:00:00
abstract::We have investigated the intracellular distribution of insoluble fibronectin in HeLa tumour cells. By indirect immunofluorescence microscopy fibronectin was detected in the nuclear region, but not in the region of the cell surface. Isolated nuclei and isolated nuclear matrices also stained for fibronectin. By quantita...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein levels of NOS2, and synth...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01002
更新日期:2004-04-01 00:00:00
abstract::The GGGGCC (G4C2) repeat expansion mutation in the C9ORF72 gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Transcription of the repeat and formation of nuclear RNA foci, which sequester specific RNA-binding proteins, is one of the possible pathological me...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.224303
更新日期:2019-03-07 00:00:00
abstract::The mammalian inactive X chromosome (Xi) is a model for facultative heterochromatin. Increased DNA compaction for the Xi, and for facultative heterochromatin in general, has long been assumed based on recognition of a distinct Barr body using nucleic-acid staining. This conclusion has been challenged by a report revea...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.026104
更新日期:2008-04-01 00:00:00
abstract::Sox7, Sox17 and Sox18 constitute group F of the Sox family of HMG box transcription factor genes. Dominant-negative mutations in Sox18 underlie the cardiovascular defects observed in ragged mutant mice. By contrast, Sox18(-/-) mice are viable and fertile, and display no appreciable anomaly in their vasculature, sugges...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03081
更新日期:2006-09-01 00:00:00
abstract::The sclerotome portion of the differentiating embryonic chick somite becomes infiltrated by neural crest cells prior to its dispersal towards the embryonic axis. This means that sclerotome cells explanted into culture for the purpose of examining their interactions in vitro are contaminated with a proportion of neural...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Immobilization of cells by specific immune serum involves crosslinking between immunoglobulin G (IgG) and the i-antigen in the cell membrane. Globular material is seen to accumulate at the ciliary tips by phase-contrast and fluorescence microscopy in a manner analogous to 'capping' in more typical eukaryotes. When imm...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::The Wnt signal transduction pathway regulates various aspects of embryonal development and is involved in cancer formation. Wnts induce the stabilisation of cytosolic (beta)-catenin, which then associates with TCF transcription factors to regulate expression of Wnt-target genes. At various levels the Wnt pathway is su...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::During the last phase of oogenesis in Drosophila, nurse cell cytoplasm can be seen to be streaming into the growing oocyte when visualized in time-lapse films. This process can be reversibly inhibited by cytochalasins. The distribution of F-actin filaments in the nurse cells has been studied by staining with rhodamine...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.049346
更新日期:2009-11-01 00:00:00
abstract::The neuroprotective role of carbon monoxide (CO) has been studied in a cell-autonomous mode. Herein, a new concept is disclosed - CO affects astrocyte-neuron communication in a paracrine manner to promote neuroprotection. Neuronal survival was assessed when co-cultured with astrocytes that had been pre-treated or not ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.187260
更新日期:2016-08-15 00:00:00
abstract::Skeletal muscle is primarily composed of large myofibers containing thousands of post-mitotic nuclei distributed throughout a common cytoplasm. Protein production and localization in specialized myofiber regions is crucial for muscle function. Myonuclei differ in transcriptional activity and protein accumulation, but ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.207670
更新日期:2018-02-05 00:00:00