Abstract:
:A key step in cell migration is the dynamic formation and disassembly of adhesions at the front and the concomitant movement and release of adhesions in the rear of the cell. Fibroblasts maintained in the absence of serum have stable adhesions within the rear of the cell and exhibit reduced trailing-edge retraction resulting in an elongated cell phenotype. Addition of lysophosphatidic acid (LPA) induced the movement of adhesions and retraction of the trailing edge, thus mimicking tail retraction in a migrating cell. Focal adhesion kinase (FAK), guanine nucleotide exchange factors (GEF) for Rho and the Rho effector Rho kinase II (ROCKII) are crucial for the regulation of adhesion movement and trailing-edge retraction. Downregulation of FAK by small interfering RNAs or small hairpin RNAs blocked LPA-induced adhesion movement and restoration of cell shape. This phenotype was rescued by the ectopic expression of PDZ-RhoGEF or a RhoA-effector-domain mutant that activates ROCK. Knockdown of PDZ-RhoGEF or ROCKII inhibited LPA-induced trailing-edge retraction and adhesion movement. Moreover, overexpressed PDZ-RhoGEF co-immunoprecipitated with FAK and localized to FAK-containing adhesions. These studies support a model in which FAK and PDZ-RhoGEF cooperate to induce Rho/ROCKII-dependent focal adhesion movement and trailing-edge retraction in response to LPA.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Iwanicki MP,Vomastek T,Tilghman RW,Martin KH,Banerjee J,Wedegaertner PB,Parsons JTdoi
10.1242/jcs.020941subject
Has Abstractpub_date
2008-03-15 00:00:00pages
895-905issue
Pt 6eissn
0021-9533issn
1477-9137pii
jcs.020941journal_volume
121pub_type
杂志文章abstract::Micrographs were made of free-swimming hamster spermatozoa using a high intensity xenon flash and two-colour darkground illumination - conditions that permit three-dimensional reconstruction of the instantaneous shape of the flagellum. From the waveforms observed, we constructed an account of the three-dimensional kin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.211433
更新日期:2018-06-27 00:00:00
abstract::The mammalian lens consists of an aged core of quiescent cells enveloped by a layer of synthetically active cells. Abundant gap junctions within and between these cell populations ensure that the lens functions as an electrical syncytium and facilitates the exchange of small molecules between surface and core cells. I...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00738
更新日期:2003-10-15 00:00:00
abstract::The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.019729
更新日期:2007-11-15 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a transcriptional regulator of genes involved in xenobiotic metabolism. Increasingly clear is also the role of the AhR in the control of cell growth and proliferation. By analyzing differential patterns of gene expression between wild-type (AhR+/+) and null (AhR-/-) mouse embryo ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00932
更新日期:2004-02-29 00:00:00
abstract::Aquaporin-4, the major water-selective channel in astroglia throughout the central nervous system, facilitates water movement into and out of the brain. Here, we identify a novel role for aquaporin-4 in astroglial cell migration, as occurs during glial scar formation. Astroglia cultured from the neocortex of aquaporin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02680
更新日期:2005-12-15 00:00:00
abstract::3-Phosphoinositide-dependent protein kinase-1 (PDK1) and phospholipase C (PLC)γ1 are two key enzymes in signal transduction that control several intracellular processes. Despite the fact that PLCγ1 has been investigated for several years, the mechanisms of activation of this enzyme are still not completely clear. Simi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.100511
更新日期:2012-07-01 00:00:00
abstract::The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Intermediate filaments (IFs) undergo specific rearrangements in cells, some aspects of which can be induced experimentally. Centripetal aggregation of the IF network, for example, can be produced by a variety of perturbations. However, the source of motive force is clear for neither in vivo nor experimentally generate...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::The small GTP-binding protein Rho rapidly stimulates the formation of focal adhesions and actin stress fibres when microinjected into serum-starved Swiss 3T3 fibroblasts. This response is inhibited by tyrosine kinase inhibitors. Addition of growth factors such as lysophosphatidic acid and bombesin to Swiss 3T3 cells s...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::During muscle development myosin molecules form symmetrical thick filaments, which integrate with the thin filaments to produce the regular sarcomeric lattice. In Drosophila indirect flight muscles (IFMs) the details of this process can be studied using genetic approaches. The weeP26 transgenic line has a GFP-encoding...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.110361
更新日期:2013-01-01 00:00:00
abstract::The ryanodine receptor (RyR) is an ion channel composed of four identical subunits mediating calcium efflux from the endo/sarcoplasmic reticulum of excitable and non-excitable cells. We present several lines of evidence indicating that the RyR2 N-terminus is capable of self-association. A combination of yeast two-hybr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.133538
更新日期:2013-11-01 00:00:00
abstract::Rubella virus (RV) envelope glycoproteins E1 and E2 are targeted to the Golgi as heterodimers. While E2 contains a transmembrane Golgi retention signal, E1 is arrested in a pre-Golgi compartment in the absence of E2, and appears to require heterodimerization in order to reach the Golgi. Various forms of E1 with deleti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::end13-1 was isolated in a screen for endocytosis mutants and has been shown to have a post-internalisation defect in endocytic transport as well as a defect in vacuolar protein sorting (Vps(-) phenotype), leading to secretion of newly synthesised vacuolar proteins. Here we demonstrate that END13 is identical to VPS4, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::We previously reported that the FLA10 locus on the uni linkage group of Chlamydomonas encodes a kinesin homologous protein, KHP1. The fla10 phenotype, which is a temperature-sensitive defect for flagellar assembly and maintenance, is rescued by transformation with the wild-type KHP1 gene. In the present study we ident...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Nedd1 is a new member of the gamma-tubulin ring complex (gammaTuRC) and targets the gammaTuRC to the centrosomes for microtubule nucleation and spindle assembly in mitosis. Although its role is known, its functional regulation mechanism remains unclear. Here we report that the function of Nedd1 is regulated by Cdk1 an...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.042747
更新日期:2009-07-01 00:00:00
abstract::Fas ligand (FasL), a potent mediator of apoptosis expressed by CTL and NK cells, is sorted into the inner vesicles of secretory lysosomes for release via exosome-like vesicles. Previous studies identified a proline-rich domain in the cytoplasmic tail required for sorting FasL to secretory lysosomes, but the mechanisms...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03315
更新日期:2007-01-01 00:00:00
abstract::Mucin expression was analysed, in relation to cell growth, in parental HT-29 cells and in two populations of mucus-secreting HT-29 cells selected by adaptation to methotrexate (HT29-MTX) or 5-fluorouracil (HT29-FU). These two populations express mature mucins that differ in their immunoreactivity to antibodies against...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed and stabilized by E-cadherin and catenin proteins linked to the actomyosin cyt...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.151472
更新日期:2014-12-15 00:00:00
abstract::Actin-filament disassembly is crucial for actin-based motility, to control filament network architecture and to regenerate subunits for assembly. Here, we examined the roles of three actin cytoskeletal proteins, coronin, cofilin and Aip1, which have been suggested to combine in various ways to control actin dynamics b...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.065698
更新日期:2010-04-15 00:00:00
abstract::Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.059634
更新日期:2010-01-15 00:00:00
abstract::We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::During mouse meiosis, the early prophase RAD51/DMC1 recombination protein sites, which are associated with the chromosome cores and which serve as markers for ongoing DNA-DNA interactions, are in ten-fold excess of the eventual reciprocal recombinant events. Most, if not all, of these early interactions are eliminated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-04-15 00:00:00
abstract::Following fertilization, there are rapid changes in the appearance of the Fucus egg. Large electron-translucent vesicles (V1) accumulate fibrillar material, and following pronuclear fusion, they are largely electron-opaque. These vesicles (V1) are formed originally in unfertilized eggs by smooth endoplasmic reticulum ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::The intraerythrocytic developmental cycle of Plasmodium falciparum is completed with the release of up to 32 invasive daughter cells, the merozoites, into the blood stream. Before release, the final step of merozoite development is the assembly of the cortical pellicle, a multi-layered membrane structure. This unique ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.181230
更新日期:2016-02-15 00:00:00
abstract::Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the beta-oxidation of fatty acids. Characterization of their properties and behavior has progressed rather slowly. However, during ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02893
更新日期:2006-03-15 00:00:00
abstract::Tetrahymena cells secrete a factor which inhibits RNA synthesis in vivo and in vitro. The factor is a relatively small peptide with a molecular weight between 300 and 1500 Daltons. Other, non-specific peptides in the broth medium or added to a chemically defined medium have a stimulatory effect on RNA synthesis in viv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::We investigated at the electron microscope level the fate of the three intranuclear structures known to accumulate snRNPs, and which correspond to the punctuate immunofluorescent staining pattern (the coiled bodies, the clusters of interchromatin granules and the interchromatin granule-associated zones) after exposure...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.163105
更新日期:2015-04-15 00:00:00