Abstract:
:We previously reported that the FLA10 locus on the uni linkage group of Chlamydomonas encodes a kinesin homologous protein, KHP1. The fla10 phenotype, which is a temperature-sensitive defect for flagellar assembly and maintenance, is rescued by transformation with the wild-type KHP1 gene. In the present study we identify the molecular defect associated with the fla10 mutation and examine the subcellular localization of KHP1 throughout the cell cycle. The mutation in the fla10-1 allele consists of a C to A transversion, which alters amino acid 329 in the motor domain of KHP1. This residue and the sequence of the carboxy-terminal third of the motor domain in which it is located are highly conserved throughout eukaryotic evolution in a subfamily of kinesin-related proteins from mouse (KIF3), sea urchin (KRP85/95), Xenopus (XKLP3), and Drosophila (KLP68D). These data suggest a conserved function for this family of proteins. Immunofluorescence studies reveal that: (1) in interphase cells KHP1 is associated with basal bodies and with the proximal portion of the flagella; (2) in cells undergoing flagellar regeneration KHP1 occurs in punctate structures that extend to the tip of the developing axoneme; and (3) in dividing cells KHP1 remains associated with centrioles throughout mitosis and localizes to the mitotic spindle. KHP1 is the first kinesin homologous protein to be found in association with basal bodies and centrioles throughout the cell cycle. These observations provide evidence for a direct role of basal bodies in the process of flagellar development, which we propose is based on KHP1 acting as a transporter of flagellar components from the basal bodies out to the distal site of assembly. The localization of KHP1 in mitosis suggests that this protein may play an analogous role in the centriole-based assembly of the mitotic spindle.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Vashishtha M,Walther Z,Hall JLsubject
Has Abstractpub_date
1996-03-01 00:00:00pages
541-9eissn
0021-9533issn
1477-9137journal_volume
109 ( Pt 3)pub_type
杂志文章abstract::Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but rep...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...
journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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doi:
更新日期:2000-08-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1994-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.150524
更新日期:2014-11-15 00:00:00
abstract::Keratins 8 and 18 (K8 and K18) are regulated by site-specific phosphorylation in response to multiple stresses. We examined the effect and regulation of hyposmotic stress on keratin phosphorylation. K8 phospho-Ser431 (Ser431-P) becomes dephosphorylated in HT29 cells, but hyperphosphorylated on other K8 but not K18 sit...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02861
更新日期:2006-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.195032
更新日期:2016-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.255422
更新日期:2021-01-25 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::Injection of the circular plasmid FV1 (derived from type I bovine papilloma virus) into Xenopus eggs before the start of the first cell cycle dramatically increases the efficiency of plasmid replication once eggs are chemically activated. We call this the preloading effect and report kinetic and quantitative character...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Two monoclonal antibodies, BA16 and BA17, have been developed using a detergent-insoluble extract of human mammary epithelial organoids as immunogen. Indirect immunofluorescent staining of cultured cells showed that the component reacting with the antibodies was filamentous and the intensity of staining was stronger i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091652
更新日期:2012-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00750
更新日期:2003-11-15 00:00:00
abstract::Vertebrate tropomyosins (TMs) are expressed from four genes, and at least 18 distinct isoforms are generated via a complex pattern of alternative RNA splicing and alternative promoters. The functional significance of this isoform diversity is largely unknown and it remains to be determined whether specific isoforms ar...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01061
更新日期:2004-04-15 00:00:00
abstract::Atomic force microscopy (AFM) was used to probe topology, conformational changes and initial substrate-carrier interactions of Na+-glucose co-transporter (SGLT1) in living cells on a single-molecule level. By scanning SGLT1-transfected Chinese hamster ovary (CHO) cells with AFM tips carrying an epitope-specific antibo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03035
更新日期:2006-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2020-07-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.064774
更新日期:2010-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Ca2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric oxide (NO) generation is dependent on Ca2+ and calmodulin (CaM). Here, we used Arabi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.201467
更新日期:2018-01-29 00:00:00
abstract::Previous investigators have examined mitochondrial DNA (mtDNA) in the electron microscope (EM) after extraction from mitochondria and rotary shadowing. We have observed mtDNA in situ by the osmiumammine procedure for specific staining of DNA in the EM. The procedure was modified to improve the regularity of the staini...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Fas ligand (FasL), a potent mediator of apoptosis expressed by CTL and NK cells, is sorted into the inner vesicles of secretory lysosomes for release via exosome-like vesicles. Previous studies identified a proline-rich domain in the cytoplasmic tail required for sorting FasL to secretory lysosomes, but the mechanisms...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03315
更新日期:2007-01-01 00:00:00
abstract::Compartmentalization of surface membrane antigens into discrete regions or domains is a characteristic feature of differentiated cells. In mammalian spermatozoa at least 5 surface domains are known, implying the presence of barriers or boundaries within the plasma membrane. Using the technique of fluorescence recovery...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::Connexin 30 (Cx30), a member of the large gap-junction protein family, plays a role in the homeostasis of the epidermis and inner ear through gap junctional intercellular communication (GJIC). Here, we investigate the underlying mechanisms of four autosomal dominant Cx30 gene mutations that are linked to hearing loss ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.138230
更新日期:2014-04-15 00:00:00
abstract::During the last phase of oogenesis in Drosophila, nurse cell cytoplasm can be seen to be streaming into the growing oocyte when visualized in time-lapse films. This process can be reversibly inhibited by cytochalasins. The distribution of F-actin filaments in the nurse cells has been studied by staining with rhodamine...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Organelle transport is vital for the development and maintenance of axons, in which the distances between sites of organelle biogenesis, function, and recycling or degradation can be vast. Movement of mitochondria in axons can serve as a general model for how all organelles move: mitochondria are easy to identify, the...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02745
更新日期:2005-12-01 00:00:00