Abstract：
:Nervous system development and function are tightly regulated by metabolic processes, including the metabolism of lipids such as fatty acids. Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic intellectual disabilities. We previously reported that Acsl, the Drosophila ortholog of mammalian ACSL3 and ACSL4, inhibits neuromuscular synapse growth by suppressing bone morphogenetic protein (BMP) signaling. Here, we report that Acsl regulates the composition of fatty acids and membrane lipids, which in turn affects neuromuscular junction (NMJ) synapse development. Acsl mutant brains had a decreased abundance of C16:1 fatty acyls; restoration of Acsl expression abrogated NMJ overgrowth and the increase in BMP signaling. A lipidomic analysis revealed that Acsl suppressed the levels of three lipid raft components in the brain, including mannosyl glucosylceramide (MacCer), phosphoethanolamine ceramide and ergosterol. The MacCer level was elevated in Acsl mutant NMJs and, along with sterol, promoted NMJ overgrowth, but was not associated with the increase in BMP signaling in the mutants. These findings suggest that Acsl inhibits NMJ growth by stimulating C16:1 fatty acyl production and concomitantly suppressing raft-associated lipid levels.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Huang Y,Huang S,Lam SM,Liu Z,Shui G,Zhang YQdoi
10.1242/jcs.195032subject
Has Abstractpub_date
2016-11-01 00:00:00pages
4034-4045issue
21eissn
0021-9533issn
1477-9137pii
jcs.195032journal_volume
129pub_type
杂志文章abstract：:The chromosomes of the active previtellogenic oocytes of the dragonfly Cordulia aenea L. extrude into the cytoplasm a substance, which is subsequently found there in the form of granulofibrillar masses. These nuclear extrusions evolve ultrastructurally into two components, nematosomes and dense masses. The nematosomes...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and intercellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how CME functions in planta To fac...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.248062
更新日期：2020-08-06 00:00:00
abstract：:Programmed cell death comprises several subtypes, as revealed by electron microscopy. Apoptosis or type I programmed cell death is characterized by condensation of cytoplasm and preservation of organelles, essentially without autophagic degradation. Autophagic cell death or type II programmed cell death exhibits exten...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:A fundamental prediction of the hypothesis that critical events in tumour formation are rare is that the tumours develop by clonal expansion. This prediction has been tested by establishing the isozyme content of tumours induced in mosaic animals whose normal tissues contain two distinguishable isozyme activities. The...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Many of the cilia- and flagella-specific integral membrane proteins identified to date function to sense the extracellular milieu, and there is considerable interest in defining pathways for targeting such proteins to these sensory organelles. The flagellar glucose transporter of Leishmania mexicana, LmxGT1, is target...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.103028
更新日期：2012-07-15 00:00:00
abstract：:Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:We have demonstrated the possibility of cross-talk between E-cadherin and alphav integrins in breast carcinoma cells. Using the function-blocking anti-alphav monoclonal antibody 17E6, applied to monolayer cultures of breast cancer lines, it was found that treatment of cells possessing detergent-insoluble (implying att...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Chick embryo fibroblasts cultivated in vitro and Ehrlich ascites tumor cells (in vivo or in vitro) have been treated with amphotericin B. Cell multiplication is strongly inhibited. Large clear zones appear in the fibroblast nucleoi (phase-contrast and electron-microscope observations). Many treated fibroblasts and tum...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body by receptor-mediated endocytosis. The accumulation of protein complexes in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03052
更新日期：2006-08-01 00:00:00
abstract：:Both at a basal level and after induction (especially in response to nutrient starvation), the function of autophagy is to allow cells to degrade and recycle damaged organelles, proteins and other biological constituents. Here, we focus on the role microtubules have in autophagosome formation, autophagosome transport ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.115626
更新日期：2013-03-01 00:00:00
abstract：:The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and thi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.030098
更新日期：2008-12-15 00:00:00
abstract：:A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:The transmembrane inner nuclear membrane (INM) protein Samp1 is required for anchoring centrosomes near the nuclei. Using high-resolution fluorescence microscopy we show that Samp1 is distributed in a distinct and characteristic pattern in the nuclear envelope (NE), where it partially colocalizes with the LINC complex...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.078923
更新日期：2011-06-15 00:00:00
abstract：:Targeting of the NG2 proteoglycan to cellular retraction fibers was studied by expressing mutant NG2 molecules lacking specific structural elements of the proteoglycan. Both the cytoplasmic domain and the chondroitin sulfate chain of NG2 appear to have roles in sorting NG2 to subcellular microdomains destined to becom...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Eukaryogenesis - the emergence of eukaryotic cells - represents a pivotal evolutionary event. With a fundamentally more complex cellular plan compared to prokaryotes, eukaryotes are major contributors to most aspects of life on Earth. For decades, we have understood that eukaryotic origins lie within both the Archaea ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.178566
更新日期：2016-10-15 00:00:00
abstract：:Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00264
更新日期：2003-02-15 00:00:00
abstract：:One aspect of human placental function which has not hitherto been studied is the ability of the placenta to digest proteins intracellularly and use the products of hydrolysis to supply its own and foetall complement of hydrolytic enzymes, including the acid proteases cathepsin C and D. We have used trophoblast cells ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Intracellular membrane fusion occurs with exquisite coordination and specificity. Each fusion event requires three basic components: Rab-GTPases organize the fusion site; SNARE proteins act during fusion; and N-ethylmaleimide-sensitive factor (NSF) plus its cofactor alpha-SNAP are required for recycling or activation ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.02561
更新日期：2005-09-01 00:00:00
abstract：:MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.101758
更新日期：2012-08-01 00:00:00
abstract：:Nemaline myopathy (NM) is a congenital myopathy with an estimated incidence of 150,000 live births. It is caused by mutations in thin filament components, including nebulin, which accounts for about 50% of the cases. The identification of NM cases with nonsense mutations resulting in loss of the extreme C-terminal SH3...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.137026
更新日期：2013-12-01 00:00:00
abstract：:Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles. LETS protein was detected by...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：:The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.019729
更新日期：2007-11-15 00:00:00
abstract：:The notion that cell shape and spreading can regulate cell proliferation has evolved over several years, but only recently has this been linked to forces from within and upon the cell. This emerging area of mechanical signaling is proving to be wide-spread and important for all cell types. The microenvironment that su...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.067009
更新日期：2011-04-15 00:00:00
abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.169466
更新日期：2015-06-15 00:00:00
abstract：:NADH:ubiquinone oxidoreductases catalyse the first step within the diverse pathways of mitochondrial NADH oxidation. In addition to the energy-conserving form commonly called complex I, fungi and plants contain much simpler alternative NADH:ubiquinone oxido-reductases that catalyze the same reaction but do not translo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00955
更新日期：2004-04-15 00:00:00
abstract：:An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.087049
更新日期：2012-03-01 00:00:00
