The changing view of eukaryogenesis - fossils, cells, lineages and how they all come together.

Abstract:

:Eukaryogenesis - the emergence of eukaryotic cells - represents a pivotal evolutionary event. With a fundamentally more complex cellular plan compared to prokaryotes, eukaryotes are major contributors to most aspects of life on Earth. For decades, we have understood that eukaryotic origins lie within both the Archaea domain and α-Proteobacteria. However, it is much less clear when, and from which precise ancestors, eukaryotes originated, or the order of emergence of distinctive eukaryotic cellular features. Many competing models for eukaryogenesis have been proposed, but until recently, the absence of discriminatory data meant that a consensus was elusive. Recent advances in paleogeology, phylogenetics, cell biology and microbial diversity, particularly the discovery of the 'Candidatus Lokiarcheaota' phylum, are now providing new insights into these aspects of eukaryogenesis. The new data have allowed the time frame during which eukaryogenesis occurred to be finessed, a more precise identification of the contributing lineages and the biological features of the contributors to be clarified. Considerable advances have now been used to pinpoint the prokaryotic origins of key eukaryotic cellular processes, such as intracellular compartmentalisation, with major implications for models of eukaryogenesis.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Dacks JB,Field MC,Buick R,Eme L,Gribaldo S,Roger AJ,Brochier-Armanet C,Devos DP

doi

10.1242/jcs.178566

subject

Has Abstract

pub_date

2016-10-15 00:00:00

pages

3695-3703

issue

20

eissn

0021-9533

issn

1477-9137

pii

jcs.178566

journal_volume

129

pub_type

杂志文章,评审
  • X-ray microanalysis of HeLa S3 Cells. I. Instrumental calibration and analysis of randomly growing cultures.

    abstract::Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Stan...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Warley A,Stephen J,Hockaday A,Appleton TC

    更新日期:1983-03-01 00:00:00

  • Partial denaturation of small chromatin fragments: direct evidence for the radial distribution of nucleosomes in folded chromatin fibers.

    abstract::To examine the internal structure of chromatin fibers, we have developed procedures for partial denaturation of small chromatin fragments (8-30 nucleosomes) from chicken erythrocytes. Electron micrographs of samples prepared under conditions that cause nucleosome dissociation show rods and loops projecting from short ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Bermúdez A,Bartolomé S,Daban JR

    更新日期:1998-06-01 00:00:00

  • The role of protein phosphorylation in the assembly of a replication competent nucleus: investigations in Xenopus egg extracts using the cyanobacterial toxin microcystin-LR.

    abstract::Cell-free extracts of Xenopus eggs support nuclear assembly and DNA replication in vitro. Extracts supplemented with the protein phosphatase inhibitor microcystin-LR displayed various inhibitory effects at different concentrations of the toxin. In the presence of cycloheximide, additions of microcystin did not induce ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Murphy J,Crompton CM,Hainey S,Codd GA,Hutchison CJ

    更新日期:1995-01-01 00:00:00

  • Function of spindle microtubules in directing cortical movement and actin filament organization in dividing cultured cells.

    abstract::The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Fishkind DJ,Silverman JD,Wang YL

    更新日期:1996-08-01 00:00:00

  • Morphological changes in frog pronephric cell surfaces after transformation by herpes virus.

    abstract::A scanning electron-microscope study has established that there are major modifications in the surface membranes of malignant cells transformed from the pronephric kidney of the frog. Tumours were induced in vivo after exposure of embryos Lucké Herpes virus. A pronephric cell line from normal embryos and 3 tumour cell...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Tweedell KS,Williams DC

    更新日期:1976-11-01 00:00:00

  • Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport.

    abstract::Sorting nexins are a large family of phox-homology-domain-containing proteins that have been implicated in the control of endosomal sorting. Sorting nexin-1 is a component of the mammalian retromer complex that regulates retrieval of the cation-independent mannose 6-phosphate receptor from endosomes to the trans-Golgi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02568

    authors: Carlton JG,Bujny MV,Peter BJ,Oorschot VM,Rutherford A,Arkell RS,Klumperman J,McMahon HT,Cullen PJ

    更新日期:2005-10-01 00:00:00

  • Activation of Rac1 by RhoG regulates cell migration.

    abstract::Cell migration is essential for normal development and many pathological processes. Rho-family small GTPases play important roles in this event. In particular, Rac regulates lamellipodia formation at the leading edge during migration. The small GTPase RhoG activates Rac through its effector ELMO and the ELMO-binding p...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02720

    authors: Katoh H,Hiramoto K,Negishi M

    更新日期:2006-01-01 00:00:00

  • A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum.

    abstract::The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible for the ingestion and transport of host cell hemoglobin to the lysosome-like food vacuol...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.023150

    authors: Lazarus MD,Schneider TG,Taraschi TF

    更新日期:2008-06-01 00:00:00

  • The mechanical integrin cycle.

    abstract::Cells govern tissue shape by exerting highly regulated forces at sites of matrix adhesion. As the major force-bearing adhesion-receptor protein, integrins have a central role in how cells sense and respond to the mechanics of their surroundings. Recent studies have shown that a key aspect of mechanotransduction is the...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.042127

    authors: Puklin-Faucher E,Sheetz MP

    更新日期:2009-01-15 00:00:00

  • Electrical dimensions in cell science.

    abstract::Cells undergo a variety of physiological processes, including division, migration and differentiation, under the influence of endogenous electrical cues, which are generated physiologically and pathologically in the extracellular and sometimes intracellular spaces. These signals are transduced to regulate cell behavio...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.023564

    authors: McCaig CD,Song B,Rajnicek AM

    更新日期:2009-12-01 00:00:00

  • Evidence for colocalization and interaction between 37 and 39 kDa isoforms of secretory carrier membrane proteins (SCAMPs).

    abstract::Secretory carrier membrane proteins (SCAMPs) are proteins of post-Golgi recycling carriers, including regulated secretory organelles. The two major size variants, SCAMP1 (37 kDa) and SCAMP2 (39 kDa), extensively colocalize in membranes of fibroblasts and parotid acinar cells based on immunocytochemistry and velocity c...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Wu TT,Castle JD

    更新日期:1997-07-01 00:00:00

  • RyR1-specific requirement for depolarization-induced Ca2+ sparks in urinary bladder smooth muscle.

    abstract::Ryanodine receptor subtype 1 (RyR1) has been primarily characterized in skeletal muscle but several studies have revealed its expression in smooth muscle. Here, we used Ryr1-null mice to investigate the role of this isoform in Ca(2+) signaling in urinary bladder smooth muscle. We show that RyR1 is required for depolar...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.009415

    authors: Fritz N,Morel JL,Jeyakumar LH,Fleischer S,Allen PD,Mironneau J,Macrez N

    更新日期:2007-11-01 00:00:00

  • Tumor necrosis factor-alpha (TNFalpha) regulates the epithelial barrier in the human intestinal cell line HT-29/B6.

    abstract::Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Schmitz H,Fromm M,Bentzel CJ,Scholz P,Detjen K,Mankertz J,Bode H,Epple HJ,Riecken EO,Schulzke JD

    更新日期:1999-01-01 00:00:00

  • Endophilin-A2-dependent tubular endocytosis promotes plasma membrane repair and parasite invasion.

    abstract::Endocytosis of caveolae has previously been implicated in the repair of plasma membrane wounds. Here, we show that caveolin-1-deficient fibroblasts lacking caveolae upregulate a tubular endocytic pathway and have a reduced capacity to reseal after permeabilization with pore-forming toxins compared with wild-type cells...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.249524

    authors: Corrotte M,Cerasoli M,Maeda FY,Andrews NW

    更新日期:2020-12-01 00:00:00

  • Chondroitin sulfate and cytoplasmic domain-dependent membrane targeting of the NG2 proteoglycan promotes retraction fiber formation and cell polarization.

    abstract::Targeting of the NG2 proteoglycan to cellular retraction fibers was studied by expressing mutant NG2 molecules lacking specific structural elements of the proteoglycan. Both the cytoplasmic domain and the chondroitin sulfate chain of NG2 appear to have roles in sorting NG2 to subcellular microdomains destined to becom...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Stallcup WB,Dahlin-Huppe K

    更新日期:2001-06-01 00:00:00

  • The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis.

    abstract::Septins have been shown to play important roles in cytokinesis in diverse organisms ranging from yeast to mammals. In this study, we show that both the unc-59 and unc-61 loci encode Caenorhabditis elegans septins. Genomic database searches indicate that unc-59 and unc-61 are probably the only septin genes in the C. el...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Nguyen TQ,Sawa H,Okano H,White JG

    更新日期:2000-11-01 00:00:00

  • NCAM is ubiquitylated, endocytosed and recycled in neurons.

    abstract::The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.019729

    authors: Diestel S,Schaefer D,Cremer H,Schmitz B

    更新日期:2007-11-15 00:00:00

  • Immune semaphorins: a new area of semaphorin research.

    abstract::The semaphorin family comprises soluble and membrane-bound proteins originally identified as axonal guidance cues that function during neuronal development. Emerging evidence suggests that a subset of semaphorins, called 'immune semaphorins', function in the immune system. The class IV semaphorins Sema4D/CD100 and Sem...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.00674

    authors: Kumanogoh A,Kikutani H

    更新日期:2003-09-01 00:00:00

  • Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts.

    abstract::Ran is an abundant GTPase of the Ras superfamily that is highly conserved in eukaryotes. In interphase cells, Ran is mainly nuclear and thought to be predominantly GTP-bound, but it is also present in the cytoplasm, probably GDP-bound. This asymmetric distribution plays an important role in directing nucleocytoplasmic...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Zhang C,Hughes M,Clarke PR

    更新日期:1999-07-01 00:00:00

  • The fusion of trout spermatozoa with Chinese hamster fibroblasts.

    abstract::Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Yip JH,Bols NC

    更新日期:1982-02-01 00:00:00

  • Tumour necrosis factor alpha confers an invasive, transformed phenotype on mammary epithelial cells.

    abstract::Although loss of cell-cell adhesion and gain of invasive properties play a crucial role in the malignant progression of epithelial tumours, the molecular signals that trigger these processes have not been fully elucidated. In light of the well-established relationship between chronic inflammation and cancer, we hypoth...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02467

    authors: Montesano R,Soulié P,Eble JA,Carrozzino F

    更新日期:2005-08-01 00:00:00

  • X-ray analysis of Lymnaea stagnalis muscle fibres does not suggest that the sarcolemma is permeable to lanthanum ions.

    abstract::The cross-striated muscle from the heart ventricle and the smooth penis retractor muscle of the freshwater snail Lymnaea stagnalis have been investigated by X-ray microanalysis to establish whether lanthanum can cross the plasma membrane, as has been reported by other investigators. Tissues were incubated in 1 mM ioni...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Swales LS,Gardner DR

    更新日期:1985-04-01 00:00:00

  • Galpha12 regulates protein interactions within the MDCK cell tight junction and inhibits tight-junction assembly.

    abstract::The polarized functions of epithelia require an intact tight junction (TJ) to restrict paracellular movement and to separate membrane proteins into specific domains. TJs contain scaffolding, integral membrane and signaling proteins, but the mechanisms that regulate TJs and their assembly are not well defined. Galpha12...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.014878

    authors: Sabath E,Negoro H,Beaudry S,Paniagua M,Angelow S,Shah J,Grammatikakis N,Yu AS,Denker BM

    更新日期:2008-03-15 00:00:00

  • Changes of the DNA content of differentiating and adult macronuclei of the ciliate Loxodes magnus (Karyorelictida).

    abstract::The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear A...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Bobyleva NN,Kudrjavtsev BN,Raikov IB

    更新日期:1980-08-01 00:00:00

  • Characterization of an SV40-transformed 3T3 cell line expressing an unusual phenotype.

    abstract::A transformed variant derived as a clone from normal 3T3 cells infected with simian virus 40 (SV40) has been found to possess a phenotype intermediate between that of normal cells and that characteristic of the transformed state, yet cells of the variant still test positively for the SV40-specific nuclear T-antigen. T...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Thompson JE,Elligsen JD,Frey HE

    更新日期:1975-08-01 00:00:00

  • Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.

    abstract::Osh6 and Osh7 are lipid transfer proteins (LTPs) that move phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM). High PS levels at the PM are key for many cellular functions. Intriguingly, Osh6 and Osh7 localize to ER-PM contact sites, although they lack membrane-targeting motifs, in...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.243733

    authors: D'Ambrosio JM,Albanèse V,Lipp NF,Fleuriot L,Debayle D,Drin G,Čopič A

    更新日期:2020-06-04 00:00:00

  • A common trafficking route for GLUT4 in cardiomyocytes in response to insulin, contraction and energy-status signalling.

    abstract::A new mouse model has been developed to study the localisation and trafficking of the glucose transporter GLUT4 in muscle. The mouse line has specific expression of a GFP and HA-epitope-tagged version of GLUT4 under the control of a muscle-specific promoter. The exofacial HA-tag has enabled fluorescent labelling of on...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.041178

    authors: Fazakerley DJ,Lawrence SP,Lizunov VA,Cushman SW,Holman GD

    更新日期:2009-03-01 00:00:00

  • NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum.

    abstract::The novel NSP gene was previously shown to encode, among a variety of neuroendocrine cell types, two 3'-overlapping transcripts, a 3.4 kb one for NSP-A (776 amino acids) and a 1.8 kb one for NSP-C (208 amino acids). The deduced proteins, which were predicted to possess distinct amino-terminal regions, appeared to exhi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: van de Velde HJ,Roebroek AJ,Senden NH,Ramaekers FC,Van de Ven WJ

    更新日期:1994-09-01 00:00:00

  • Cdc14: a highly conserved family of phosphatases with non-conserved functions?

    abstract::CDC14 was originally identified by L. Hartwell in his famous screen for genes that regulate the budding yeast cell cycle. Subsequent work showed that Cdc14 belongs to a family of highly conserved dual-specificity phosphatases that are present in a wide range of organisms from yeast to human. Human CDC14B is even able ...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.074815

    authors: Mocciaro A,Schiebel E

    更新日期:2010-09-01 00:00:00

  • Involvement of intracellular calcium in protein secretion in Dictyostelium discoideum.

    abstract::We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Coukell MB,Cameron AM,Adames NR

    更新日期:1992-10-01 00:00:00