Abstract：
:Septins have been shown to play important roles in cytokinesis in diverse organisms ranging from yeast to mammals. In this study, we show that both the unc-59 and unc-61 loci encode Caenorhabditis elegans septins. Genomic database searches indicate that unc-59 and unc-61 are probably the only septin genes in the C. elegans genome. UNC-59 and UNC-61 localize to the leading edge of cleavage furrows and eventually reside at the midbody. Analysis of unc-59 and unc-61 mutants revealed that each septin requires the presence of the other for localization to the cytokinetic furrow. Surprisingly, unc-59 and unc-61 mutants generally have normal embryonic development; however, defects were observed in post-embryonic development affecting the morphogenesis of the vulva, male tail, gonad, and sensory neurons. These defects can be at least partially attributed to failures in post-embryonic cytokineses although our data also suggest other possible roles for septins. unc-59 and unc-61 double mutants show similar defects to each of the single mutants.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Nguyen TQ,Sawa H,Okano H,White JGkeywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
3825-37eissn
0021-9533issn
1477-9137journal_volume
113 Pt 21pub_type
杂志文章abstract：:Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.213496
更新日期：2019-07-30 00:00:00
abstract：:As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its sy...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Sonic hedgehog (Shh) and its patched-smoothened receptor complex control a variety of functions in the developing central nervous system, such as neural cell proliferation and differentiation. Recently, Shh signaling components have been found to be expressed at the synaptic level in the postnatal brain, suggesting a ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.247700
更新日期：2020-10-23 00:00:00
abstract：:Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.01558
更新日期：2004-11-15 00:00:00
abstract：:Survivin, a human inhibitor of apoptosis protein (IAP), plays an important role in both cell cycle regulation and inhibition of apoptosis. Survivin is expressed in cells during the G(2)/M phase of the cell cycle, followed by rapid decline of both mRNA and protein levels at the G(1) phase. It has been suggested that ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Autophagy is fundamental for cell and organismal health. Two types of autophagy are conserved in eukaryotes: macroautophagy and microautophagy. During macroautophagy, autophagosomes deliver cytoplasmic constituents to endosomes or lysosomes, whereas during microautophagy lytic organelles take up cytoplasm directly. Wh...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.246322
更新日期：2020-09-09 00:00:00
abstract：:The Birt-Hogg-Dubé (BHD) syndrome related protein FLCN has recently been implicated in the vesicular trafficking processes by interacting with several Rab family GTPases. In the previous studies, we have shown that FLCN could inhibit the binding of overexpressed PAT1, which is a membrane-bound amino acid transporter, ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.218792
更新日期：2018-12-19 00:00:00
abstract：:The outer nuclear membrane proteins nesprin-1 and nesprin-2 are retained at the nuclear envelope through an interaction of their klarsicht/ANC-1/syne homology (KASH) domain with Sun proteins present at the inner nuclear membrane. We investigated the requirements for the localization of nesprin-3alpha at the outer nucl...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.014191
更新日期：2007-10-01 00:00:00
abstract：:Pregnancy-associated plasma protein A2 (PAPP-A2, also known as pappalysin-2) is a large metalloproteinase that is known to be required for normal postnatal growth and bone development in mice. We here report the detection of zebrafish papp-a2 mRNA in the chordamesoderm, notochord and lower jaw of zebrafish (Danio reri...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.152587
更新日期：2014-12-01 00:00:00
abstract：:During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:The efficient functioning of striated muscle is dependent upon the structure of several cytoskeletal networks including myofibrils, microtubules, and intermediate filaments. However, little is known about how these networks function together during muscle differentiation and maintenance. In vitro studies suggest that ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01158
更新日期：2004-07-01 00:00:00
abstract：:Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.075275
更新日期：2010-11-01 00:00:00
abstract：:Vertebrate tropomyosins (TMs) are expressed from four genes, and at least 18 distinct isoforms are generated via a complex pattern of alternative RNA splicing and alternative promoters. The functional significance of this isoform diversity is largely unknown and it remains to be determined whether specific isoforms ar...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:The 43 kDa inositol polyphosphate 5-phosphatase (5-phosphatase) hydrolyses the signalling molecules inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3)) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4, 5)P(4)) in a signal-terminating reaction. We have utilised cell lines that stably underexpress the 43 kDa 5-phosphatase, a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Endocytosis is a regulated physiological process by which membrane receptors and their extracellular ligands are internalized. After internalization, they enter the endosomal trafficking pathway for sorting and processing. Amphiphysins consist of a family of proteins conserved throughout evolution that are crucial ele...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00403
更新日期：2003-05-15 00:00:00
abstract：:Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed and stabilized by E-cadherin and catenin proteins linked to the actomyosin cyt...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.151472
更新日期：2014-12-15 00:00:00
abstract：:The microtubule-associated protein tau interacts with the SH3 domain of non-receptor Src family protein tyrosine kinases. A potential consequence of the SH3 interaction is the upregulation of tyrosine kinase activity. Here we investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03378
更新日期：2007-03-01 00:00:00
abstract：:Filamin (actin-binding protein) is a cytoskeletal protein that crosslinks actin filaments in vitro. Filamin is thought to be involved in a variety of cell types in stabilizing actin networks, and in platelets it may play a role in linking actin to the membrane. In this report, we describe a monoclonal antibody (Mab 6E...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:The cellular microenvironment, characterized by an extracellular matrix (ECM), played an essential role in the transition from unicellularity to multicellularity in animals (metazoans), and in the subsequent evolution of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily -...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.203950
更新日期：2018-04-09 00:00:00
abstract：:Although changes in mitochondrial size and arrangement accompany both cellular differentiation and human disease, the mechanisms that mediate mitochondrial fusion, fission and morphogenesis in mammalian cells are not understood. We have identified two human genes encoding potential mediators of mitochondrial fusion. T...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA. Using a double-labelling procedure we have compared, at different salt concentrations, t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Actin functions in a multitude of cellular processes owing to its ability to polymerize into filaments, which can be further organized into higher-order structures by an array of actin-binding and regulatory proteins. Therefore, research on actin and actin-related functions relies on the visualization of actin structu...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.189068
更新日期：2017-02-01 00:00:00
abstract：:If mitotic-arrested, cold-stored HeLa cells are incubated at 37 degrees C a proportion of the population divides by an aberrant process which we have called subdivision by extrusion. This process has been studied by time-lapse photography and shown to differ from normal cleavage in several respects. The cell surface b...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Electron-microscope autoradiography has been used to obtain information on the localization of DNA labelled with [3H]thymidine in chloroplasts known to be replicating and concomitantly synthesizing and segregating DNA, in cultured leaf disks. The studies were made using both Microdol-X developer and a 'compact' develo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:Phosphoinositides regulate numerous processes in various subcellular compartments. Whereas many stimuli trigger changes in the plasma-membrane PtdIns(4,5)P(2) concentration, little is known about its precursor, PtdIns(4)P, in particular whether there are stimulus-induced alterations independent of those of PtdIns(4,5)...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.060525
更新日期：2010-05-01 00:00:00
abstract：:Previous studies have shown that the non-alpha-helical, amino-terminal head region of vimentin is essential for the formation and stability of vimentin intermediate filaments (IFs). In order to specify its target site on companion protein subunits, it was cut off from vimentin at amino acid position 96 with lysine-spe...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:To examine the role of the keratin head region and its subdomains in filament assembly we constructed several deletion mutants of type I and type II keratins and analysed their in vitro IF forming capacity. The delta K8 (1-74) and delta K18 (1-56), mutants formed only soluble oligomers, predominantly tetramers with th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Basement membranes are complex extracellular matrices contributing to the regulation of growth, migration and differentiation of many cell types. However, little is known about the mechanisms regulating the deposition and assembly of basement membrane from its constituents. We have investigated the role of extracellul...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The ret finger protein (rfp) is a member of the B-box zinc finger gene family many of which may function in growth regulation and in the appropriate context become oncogenic. Members of this family are nuclear proteins that possess a characteristic tripartite motif consisting of the RING and B-box zinc binding domains...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:During mouse meiosis, the early prophase RAD51/DMC1 recombination protein sites, which are associated with the chromosome cores and which serve as markers for ongoing DNA-DNA interactions, are in ten-fold excess of the eventual reciprocal recombinant events. Most, if not all, of these early interactions are eliminated...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-04-15 00:00:00
