Abstract:
:The microtubule-associated protein tau interacts with the SH3 domain of non-receptor Src family protein tyrosine kinases. A potential consequence of the SH3 interaction is the upregulation of tyrosine kinase activity. Here we investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased the kinase activity in in vitro assays and in transfected COS7 cells. In platelet-derived growth factor (PDGF)-stimulated fibroblasts, tau appeared to prime Src for activation following PDGF stimulation, as reflected by changes in Src-mediated actin rearrangements. In addition, while fibroblasts normally recovered actin stress fibers by 5-7 hours after PDGF stimulation, tau-expressing cells showed sustained actin breakdown. Microtubule association by tau was not required for the observed changes in actin morphology. Inhibition of Src kinases or a mutant deficient in Src interaction reduced the effects, implicating Src family protein tyrosine kinases as a mediator of the effects of tau on actin rearrangements. Our results provide evidence that the interaction of tau with Src upregulates tyrosine kinase activity and that this interaction allows tau to impact on growth-factor-induced actin remodeling.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Sharma VM,Litersky JM,Bhaskar K,Lee Gdoi
10.1242/jcs.03378subject
Has Abstractpub_date
2007-03-01 00:00:00pages
748-57issue
Pt 5eissn
0021-9533issn
1477-9137pii
jcs.03378journal_volume
120pub_type
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2015-05-15 00:00:00
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