Distinct plasma-membrane PtdIns(4)P and PtdIns(4,5)P2 dynamics in secretagogue-stimulated beta-cells.

abstract：:The mAb RK7, previously shown to recognize keratin 19, was also found to cross-react with a biologically unrelated 102 kDa protein, which becomes associated with the poles of the mitotic apparatus. This newly identified protein, called cytocentrin, is a stable cellular component, may be at least in part phosphorylated...

journal_title：Journal of cell science

authors： Paul EC,Quaroni A

更新日期：1993-11-01 00:00:00

abstract：:Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...

journal_title：Journal of cell science

authors： Xin X,Ferraro F,Bäck N,Eipper BA,Mains RE

更新日期：2004-09-15 00:00:00

abstract：:Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only c...

journal_title：Journal of cell science

authors： Dan P,Velot É,Decot V,Menu P

更新日期：2015-07-15 00:00:00

abstract：:Quantitative studies on the adhesive properties of transformed cells have yielded inconclusive and sometimes contradictory results. The present investigation has examined adhesive interactions between normal human fibroblasts, established as well as virus-transformed animal cell lines, and human tumour-derived cell li...

journal_title：Journal of cell science

authors： Brugmans M,Cassiman JJ,van den Berghe H

更新日期：1978-10-01 00:00:00

abstract：:The conventional kinesin motor transports many different cargos to specific locations in neurons. How cargos regulate motor function remains unclear. Here we focus on KIF5, the heavy chain of conventional kinesin, and report that the Kv3 (Shaw) voltage-gated K(+) channel, the only known tetrameric KIF5-binding protein...

journal_title：Journal of cell science

authors： Barry J,Xu M,Gu Y,Dangel AW,Jukkola P,Shrestha C,Gu C

更新日期：2013-05-01 00:00:00

abstract：:Many of the cilia- and flagella-specific integral membrane proteins identified to date function to sense the extracellular milieu, and there is considerable interest in defining pathways for targeting such proteins to these sensory organelles. The flagellar glucose transporter of Leishmania mexicana, LmxGT1, is target...

journal_title：Journal of cell science

authors： Tran KD,Rodriguez-Contreras D,Shinde U,Landfear SM

更新日期：2012-07-15 00:00:00

abstract：:The extracellular environment and tissue architecture contribute to proper cell function and growth control. Cells growing in monolayers on standard polystyrene tissue culture plates differ in their shape, growth rate and response to external stimuli, compared with cells growing in vivo. Here, we showed that the EGFR ...

journal_title：Journal of cell science

authors： Mizushima H,Wang X,Miyamoto S,Mekada E

更新日期：2009-12-01 00:00:00

abstract：:The cohesin complex prevents premature segregation of duplicated chromosomes by providing resistance to the pole-ward pull by spindle microtubules. The centromeric region (or sister kinetochores) bears the majority of this force and undergoes transient separation prior to anaphase, indicative of its elastic nature. A ...

journal_title：Journal of cell science

authors： Zhang T,Lim HH,Cheng CS,Surana U

更新日期：2006-02-01 00:00:00

abstract：:Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhibition of cyclin B-cdc2 kinase activation by unreplicated DNA. In sea urchin, fertilization stimulates protein synthesis and releases eggs from G1 arrest. We found that in the one-cell sea urchin embryo cyclin B-cd...

journal_title：Journal of cell science

authors： Genevière-Garrigues AM,Barakat A,Dorée M,Moreau JL,Picard A

更新日期：1995-07-01 00:00:00

abstract：:Cutaneous wound healing is accelerated by exogenous mechanical forces and is impaired in TRPC6-knockout mice. Therefore, we designed experiments to determine how mechanical force and TRPC6 channels contribute to wound healing using HaCaT keratinocytes. HaCaT cells were pretreated with hyperforin, a major component of ...

journal_title：Journal of cell science

authors： Takada H,Furuya K,Sokabe M

更新日期：2014-10-01 00:00:00

abstract：:Protrusion formation is the first step that precedes cell movement of motile cells. Spatial control of actin polymerization is necessary to achieve directional protrusion during cell migration. Here we show that the spatial coordinators p190RhoGEF and p190RhoGAP regulate actin polymerization during leading edge protru...

journal_title：Journal of cell science

authors： Bravo-Cordero JJ,Sharma VP,Roh-Johnson M,Chen X,Eddy R,Condeelis J,Hodgson L

更新日期：2013-08-01 00:00:00

abstract：:The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...

journal_title：Journal of cell science

authors： Hetheridge C,Scott AN,Swain RK,Copeland JW,Higgs HN,Bicknell R,Mellor H

更新日期：2012-03-15 00:00:00

abstract：:Cultured human erythrocytes infected with Plasmodium falciparum were studied by freeze-fracture electron microscopy. Special emphasis was placed upon the formation of the membrane surface excrescences ('knobs') found on red cells containing mature parasites. Knobs were visualized as conoid projections of the protoplas...

journal_title：Journal of cell science

authors： Allred DR,Gruenberg JE,Sherman IW

更新日期：1986-03-01 00:00:00

abstract：:The mixed lineage leukemia (MLL) proto-oncogenic protein is a histone-lysine N-methyltransferase that is produced by proteolytic cleavage and self-association of the respective functionally distinct subunits (MLL(N) and MLL(C)) to form a holocomplex involved in epigenetic transcriptional regulation. On the basis of st...

journal_title：Journal of cell science

authors： Yokoyama A,Ficara F,Murphy MJ,Meisel C,Naresh A,Kitabayashi I,Cleary ML

更新日期：2011-07-01 00:00:00

abstract：:It has been shown previously that whilst the rat type I integral membrane protein TGN38 (ratTGN38) is predominantly localised to the trans-Golgi network this protein does reach the cell surface from where it is internalised and delivered back to the trans-Golgi network. This protein thus provides a suitable tool for t...

journal_title：Journal of cell science

authors： Banting G,Maile R,Roquemore EP

更新日期：1998-12-01 00:00:00

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:The polarized functions of epithelia require an intact tight junction (TJ) to restrict paracellular movement and to separate membrane proteins into specific domains. TJs contain scaffolding, integral membrane and signaling proteins, but the mechanisms that regulate TJs and their assembly are not well defined. Galpha12...

journal_title：Journal of cell science

authors： Sabath E,Negoro H,Beaudry S,Paniagua M,Angelow S,Shah J,Grammatikakis N,Yu AS,Denker BM

更新日期：2008-03-15 00:00:00

abstract：:Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...

journal_title：Journal of cell science

authors： Liu H,Chen SE,Jin B,Carson JA,Niu A,Durham W,Lai JY,Li YP

更新日期：2010-09-01 00:00:00

abstract：:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...

journal_title：Journal of cell science

authors： Paladino S,Lebreton S,Tivodar S,Campana V,Tempre R,Zurzolo C

更新日期：2008-12-15 00:00:00

abstract：:The Dlg tumour suppressor protein is intimately involved in the control of cell contact and polarity. Previous studies have shown that hDlg is a target for a number of viral transforming proteins. In particular, the high risk human papillomavirus (HPV) E6 proteins target hDlg for proteasome-mediated degradation, an ac...

journal_title：Journal of cell science

authors： Mantovani F,Massimi P,Banks L

更新日期：2001-12-01 00:00:00

abstract：:Diversity of cell lineages within glandular organs is generated postnatally by differentiation of committed progenitor cells. Fundamental regulatory aspects of this process are not understood. The mouse submandibular salivary gland (SSG) served as model to assess the role of epidermal growth factor (EGF) receptor sign...

journal_title：Journal of cell science

authors： Durban EM,Nagpala PG,Barreto PD,Durban E

更新日期：1995-06-01 00:00:00

abstract：:We have used genetic and molecular techniques to investigate the interactions among genes required for the initiation and regulation of septum formation in Schizosaccharomyces pombe. Our data suggest that the products of the cdc7, cdc11, cdc14 and cdc16 genes interact. These activities may regulate the function of the...

journal_title：Journal of cell science

authors： Marks J,Fankhauser C,Simanis V

更新日期：1992-04-01 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Direct-current electric fields mediate motility (galvanotaxis) of many cell types. In 3T3 fibroblasts, electric fields increased the proportion, speed and cathodal directionality of motile cells. Analogous to fibroblasts' spontaneous migration, we initially hypothesized that reorientation of microtubule components mod...

journal_title：Journal of cell science

authors： Finkelstein E,Chang W,Chao PH,Gruber D,Minden A,Hung CT,Bulinski JC

更新日期：2004-03-15 00:00:00

abstract：:ATP-dependent priming of the secretory granules precedes Ca(2+)-regulated neuroendocrine secretion, but the exact nature of this reaction is not fully established in all secretory cell types. We have further investigated this reaction in the insulin-secreting pancreatic B-cell and demonstrate that granular acidificati...

journal_title：Journal of cell science

authors： Barg S,Huang P,Eliasson L,Nelson DJ,Obermüller S,Rorsman P,Thévenod F,Renström E

更新日期：2001-06-01 00:00:00

abstract：:The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. L...

journal_title：Journal of cell science

authors： Beesley JE,Pearson JD,Carleton JS,Hutchings A,Gordon JL

更新日期：1978-10-01 00:00:00

abstract：:DNA replication in the polytene chromosomes of larval salivary glands of Drosophila hydei has been examined under normal physiological conditions, with or without puromycin, by an autoradiographic procedure using [3H]thymidine. It has been demonstrated that puromycin induces new labelling patterns that can be identifi...

journal_title：Journal of cell science

authors： Mukherjee AS,Chatterjee C

更新日期：1984-01-01 00:00:00

abstract：:E-cadherin is the main cell adhesion molecule of early embryonic and adult epithelial cells. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic mesoderm formation and tumor progression. To identify genes whose expression is affected by the loss of E-cadherin, we compared...

journal_title：Journal of cell science

authors： Orsulic S,Kemler R

更新日期：2000-05-01 00:00:00

abstract：:During gestation the epidermis develops from a single layer of ectoderm into a layer of keratinocytes overlaid by a layer of periderm; this is followed by a progressive increase in the number of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are for...

journal_title：Journal of cell science

authors： Watt FM,Keeble S,Fisher C,Hudson DL,Codd J,Salisbury JR

更新日期：1989-10-01 00:00:00

abstract：:The regulation of mitochondrial dynamics by the GTPase OPA1, which is located at the inner mitochondrial membrane, is crucial for adapting mitochondrial function and preserving cellular health. OPA1 governs the delicate balance between fusion and fission in the dynamic mitochondrial network. A disturbance of this bala...

journal_title：Journal of cell science

authors： MacVicar T,Langer T

更新日期：2016-06-15 00:00:00