Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification.

abstract：:Studies of cellular function in the respiratory tract lining have traditionally been limited by the small tissue mass and functional diversity of the epithelium. Recent improvements in culture conditions have permitted long-term maintenance of epithelial cells derived from the upper respiratory tract of rats. The pres...

journal_title：Journal of cell science

authors： Heckman CA,Marchok AC,Nettesheim P

更新日期：1978-08-01 00:00:00

abstract：:Different isoforms of non-muscle myosin II have different distributions in vivo, even within individual cells. In order to understand how these different distributions arise, the distribution and dynamics of non-muscle myosins IIA and myosin IIB were examined in cultured cells using immunofluorescence staining and tim...

journal_title：Journal of cell science

authors： Kolega J

更新日期：1998-08-01 00:00:00

abstract：:When green cells of Euglena gracilis, strain Z, were light-grown for several months on a solid medium containing an excess of sodium acetate (1.0% instead of the normal 0.1%), some 30% of the cells were colourless. The 'acetate-bleached' organisms, isolated by plating methods and subsequently incubated in the light in...

journal_title：Journal of cell science

authors： Vannini GL

更新日期：1983-05-01 00:00:00

abstract：:Trypsin digestion of demembranated fowl spermatozoa caused a longitudinal splitting of the distal part of the axoneme. The resulting strands, consisting of groups of doublet microtubules, formed left-handed helices. On the evidence of electron micrographs, the digestion had caused the loss of dynein arms from the oute...

journal_title：Journal of cell science

authors： Woolley DM,Brammall A

更新日期：1987-10-01 00:00:00

abstract：:The development or maturation of intercellular adhesions following their initiation has received very little attention even though this is an area of significance for a variety of in vivo processes. Using Ca2(+)-induced desmosome formation in MDCK cells as a study system it is shown that, following its initiation, des...

journal_title：Journal of cell science

authors： Mattey DL,Burdge G,Garrod DR

更新日期：1990-12-01 00:00:00

abstract：:A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...

journal_title：Journal of cell science

authors： Auckland P,McAinsh AD

更新日期：2015-09-15 00:00:00

abstract：:IkappaB are responsible for maintaining p65 in the cytoplasm under non-stimulating conditions and promoting the active export of p65 from the nucleus following NFkappaB activation to terminate the signal. We now show that 14-3-3 proteins regulate the NFkappaB signaling pathway by physically interacting with p65 and Ik...

journal_title：Journal of cell science

authors： Aguilera C,Fernández-Majada V,Inglés-Esteve J,Rodilla V,Bigas A,Espinosa L

更新日期：2006-09-01 00:00:00

abstract：:p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and mei...

journal_title：Journal of cell science

authors： Sasagawa Y,Otani M,Higashitani N,Higashitani A,Sato K,Ogura T,Yamanaka K

更新日期：2009-10-15 00:00:00

abstract：:During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...

journal_title：Journal of cell science

authors： Faruki S,Dorée M,Karsenti E

更新日期：1992-01-01 00:00:00

abstract：:Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...

journal_title：Journal of cell science

authors： Baschieri F,Le Devedec D,Tettarasar S,Elkhatib N,Montagnac G

更新日期：2020-09-01 00:00:00

abstract：:We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...

journal_title：Journal of cell science

authors： Andreassen PR,Palmer DK,Wener MH,Margolis RL

更新日期：1991-07-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate [PtdIns(3)P] regulates endocytic and autophagic membrane traffic. In order to understand the downstream effects of PtdIns(3)P in these processes, it is important to identify PtdIns(3)P-binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYV...

journal_title：Journal of cell science

authors： Simonsen A,Birkeland HC,Gillooly DJ,Mizushima N,Kuma A,Yoshimori T,Slagsvold T,Brech A,Stenmark H

更新日期：2004-08-15 00:00:00

abstract：:To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppr...

journal_title：Journal of cell science

authors： Nakano M,Okamoto Y,Ohzeki J,Masumoto H

更新日期：2003-10-01 00:00:00

abstract：:It has been suggested by many studies that Ca2+ signaling plays an important role in regulating key steps in cell division. In order to study the down stream components of calcium signaling, we have fused the gene of calmodulin (CaM) with that of green fluorescent protein (GFP) and expressed it in HeLa cells. The GFP-...

journal_title：Journal of cell science

authors： Li CJ,Heim R,Lu P,Pu Y,Tsien RY,Chang DC

更新日期：1999-05-01 00:00:00

abstract：:The protein product of the gene responsible for ocular albinism type 1, named OA1, is a pigment-cell-specific membrane glycoprotein, displaying features of G-protein-coupled receptors, yet exclusively localized to late endosomes, lysosomes and melanosomes. To dissect the signals responsible for the intracellular local...

journal_title：Journal of cell science

authors： Piccirillo R,Palmisano I,Innamorati G,Bagnato P,Altimare D,Schiaffino MV

更新日期：2006-05-15 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:Asymmetric dimethylarginine (ADMA) is an inhibitor of nitric oxide production associated with abnormal blood vessel growth and repair, however, the mechanism of action of ADMA is not well understood. We studied the role of exogenous and endogenous ADMA in the regulation of cell motility and actin cytoskeleton in porci...

journal_title：Journal of cell science

authors： Wojciak-Stothard B,Torondel B,Tsang LY,Fleming I,Fisslthaler B,Leiper JM,Vallance P

更新日期：2007-03-15 00:00:00

abstract：:Semaphorin 3A (Sema3A), a secretory semaphorin, exerts various biological actions through a complex between neuropilin-1 and plexin-As (PlexAs). Sema3A induces retrograde signaling, which is involved in regulating dendritic localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a ...

journal_title：Journal of cell science

authors： Yamashita N,Yamane M,Suto F,Goshima Y

更新日期：2016-05-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illu...

journal_title：Journal of cell science

authors： Serrano A,Illgen J,Brandt U,Thieme N,Letz A,Lichius A,Read ND,Fleißner A

更新日期：2018-05-04 00:00:00

abstract：:The nuclear envelope is much more than a simple barrier between nucleoplasm and cytoplasm. Nuclear envelope bridging complexes are protein complexes spanning both the inner and outer nuclear envelope membranes, thus directly connecting the cytoplasm with the nucleoplasm. In metazoans, they are involved in connecting t...

journal_title：Journal of cell science

authors： Meier I

更新日期：2016-10-01 00:00:00

abstract：:It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global tra...

journal_title：Journal of cell science

authors： Stonyte V,Boye E,Grallert B

更新日期：2018-09-03 00:00:00

abstract：:Integral membrane and secretory proteins which fail to fold productively are retained in the endoplasmic reticulum and targeted for degradation by cytoplasmic proteasomes. Genetic and biochemical analyses suggest that substrates of this pathway must be dislocated across the membrane of the endoplasmic reticulum (ER) b...

journal_title：Journal of cell science

authors： Lenk U,Yu H,Walter J,Gelman MS,Hartmann E,Kopito RR,Sommer T

更新日期：2002-07-15 00:00:00

abstract：:The change of the nuclear pore frequency during the nuclear cycle of synchronous plasmodia of Physaru- polycephalum was investigated. Counts were made on platinum-carbon replicas of isolated nuclei. Pore numbers varied markedly at any given time of the nuclear cycle, possibly due to the variable DNA contents of the nu...

journal_title：Journal of cell science

authors： Schel JH,Steenbergen LC,Bekers AG,Wanka F

更新日期：1978-12-01 00:00:00

abstract：:Ferlin proteins mediate membrane-fusion events in response to Ca(2+). Myoferlin, a member of the ferlin family, is required for normal muscle development, during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection...

journal_title：Journal of cell science

authors： Demonbreun AR,Lapidos KA,Heretis K,Levin S,Dale R,Pytel P,Svensson EC,McNally EM

更新日期：2010-07-15 00:00:00

abstract：:A conjugate of horseradish peroxidase (HRP) to poly(L-lysine) (PLL) was used as a non-specific adsorptive probe to study transcytosis in MDCK strain I and Caco-2 epithelial cells. As we have shown previously, HRP-PLL transcytosis proceeds via an intracellular, non-lysosomal proteolytic compartment in MDCK cells; yet, ...

journal_title：Journal of cell science

authors： Taub ME,Shen WC

更新日期：1993-12-01 00:00:00

abstract：:Endothelial cells (ECs) sense shear stress and transduce blood flow information into functional responses that play important roles in vascular homeostasis and pathophysiology. A unique feature of shear-stress-sensing is the involvement of many different types of membrane-bound molecules, including receptors, ion chan...

journal_title：Journal of cell science

authors： Yamamoto K,Ando J

更新日期：2013-03-01 00:00:00

abstract：:High-throughput neurotransmission at ribbon synapses of cochlear inner hair cells (IHCs) requires tight coupling of neurotransmitter release and balanced recycling of synaptic vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) fo...

journal_title：Journal of cell science

authors： Kroll J,Özçete ÖD,Jung S,Maritzen T,Milosevic I,Wichmann C,Moser T

更新日期：2020-01-22 00:00:00

abstract：:The Sar1 GTPase controls coat assembly on coat protein complex II (COPII)-coated vesicles, which mediate protein transport from the endoplasmic reticulum (ER) to the Golgi. The GTP-bound form of Sar1, activated by the ER-localized guanine nucleotide exchange factor (GEF) Sec12, associates with the ER membrane. GTP hyd...

journal_title：Journal of cell science

authors： Kurokawa K,Suda Y,Nakano A

更新日期：2016-09-01 00:00:00

abstract：:We have cloned and characterized a single copy C. reinhardtii gene containing an open reading frame of 333 nucleotides encoding a 12.7 kDa protein. The novel protein, DIP13, exhibits 60% identity with two mammalian proteins, human NA14 and an unnamed mouse protein. Homologous sequences are also present in several prot...

journal_title：Journal of cell science

authors： Pfannenschmid F,Wimmer VC,Rios RM,Geimer S,Kröckel U,Leiherer A,Haller K,Nemcová Y,Mages W

更新日期：2003-04-15 00:00:00

abstract：:In neutrophils and other phagocytic cells, the small GTPase Rac is an essential regulator of a multi-component NADPH oxidase that produces high levels of superoxide, which kills invading pathogens. In many other cell types, Rac and newly discovered relatives of the neutrophil burst oxidase and its subunits have been f...

journal_title：Journal of cell science

authors： Werner E

更新日期：2004-01-15 00:00:00