GTPases and reactive oxygen species: switches for killing and signaling.

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:The effect of a synthetic peptide consisting of Arg-Gly-Asp-Ser (RGDS), the amino acid sequence representing the fibroblast attachment site in fibronectin (FN), was tested on migrating newt epidermal cells. In one approach, skin explants were placed on the bottom of plastic dishes coated with human FN, human fibrinoge...

journal_title：Journal of cell science

authors： Donaldson DJ,Mahan JT,Smith GN Jr

更新日期：1987-05-01 00:00:00

abstract：:Intermediate filaments (IFs) undergo specific rearrangements in cells, some aspects of which can be induced experimentally. Centripetal aggregation of the IF network, for example, can be produced by a variety of perturbations. However, the source of motive force is clear for neither in vivo nor experimentally generate...

journal_title：Journal of cell science

authors： Tint IS,Hollenbeck PJ,Verkhovsky AB,Surgucheva IG,Bershadsky AD

更新日期：1991-03-01 00:00:00

abstract：:Multivesicular endosomes are present in virtually every eucaryotic cell, where they arise by intra-endosomal budding of the limiting endosomal membrane. Some genetic diseases such as Chediak-Higashi syndrome are characterized by enlarged membrane-filled endosomes. The same altered endosomal morphology can be observed ...

journal_title：Journal of cell science

authors： Marchetti A,Mercanti V,Cornillon S,Alibaud L,Charette SJ,Cosson P

更新日期：2004-12-01 00:00:00

abstract：:We report a fast method for the isolation of homogeneous C-protein from bovine skeletal muscle. In electron micrographs C-protein appears as short rods with a relatively uniform length of about 50 nm. Protein sequencing shows a single N-terminal sequence. Radio-labelled C-protein strongly decorates titin II and myosin...

journal_title：Journal of cell science

authors： Fürst DO,Vinkemeier U,Weber K

更新日期：1992-08-01 00:00:00

abstract：:Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...

journal_title：Journal of cell science

authors： Afonso C,Henrique D

更新日期：2006-10-15 00:00:00

abstract：:Glutathione (GSH) levels progressively decline during aging and in neurodegenerative disorders. However, the contribution of such event in mediating neuronal cell death is still uncertain. In this report, we show that, in neuroblastoma cells as well as in primary mouse cortical neurons, GSH decrease, induced by buthio...

journal_title：Journal of cell science

authors： Aquilano K,Baldelli S,Cardaci S,Rotilio G,Ciriolo MR

更新日期：2011-04-01 00:00:00

abstract：:Two of the best understood somatic cell mRNA cytoplasmic trafficking elements are those governing localization of beta-actin and myelin basic protein mRNAs. These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively. It is not known whether these elements fulfil other roles in ...

journal_title：Journal of cell science

authors： Snee M,Kidd GJ,Munro TP,Smith R

更新日期：2002-12-01 00:00:00

abstract：:Dystrophin has been proposed to associate with the skeletal muscle membrane by way of a glycoprotein complex that interacts with its C-terminal domains. Transfection of mdx mouse myotubes in culture or myofibres in vivo with recombinant genes encoding human dystrophin deletion mutants shows, however, that not only the...

journal_title：Journal of cell science

authors： Dunckley MG,Wells KE,Piper TA,Wells DJ,Dickson G

更新日期：1994-06-01 00:00:00

abstract：:Axenically grown cells of D. discoideum Ax-2 harvested in the log phase of growth, cohere rapidly when shaken in phosphate buffer. After 3.5 days in the stationary phase of growth, cells become completely non-cohesive. Although they do not stick to each other, stationary phase cells do stick to both log phase cells an...

journal_title：Journal of cell science

authors： Jaffe AR,Swan AP,Garrod DR

更新日期：1979-06-01 00:00:00

abstract：:A new aspect of research into the pathogenesis of cystic fibrosis (CF) is a genetics-based search for ;modifier genes' that may affect the severity of CF lung disease. Using an alternative, cell biological approach, we show that ITPK1 should be considered a modifier gene. ITPK1 synthesizes an intracellular signal, ino...

journal_title：Journal of cell science

authors： Yang L,Reece J,Gabriel SE,Shears SB

更新日期：2006-04-01 00:00:00

abstract：:The semaphorin family comprises soluble and membrane-bound proteins originally identified as axonal guidance cues that function during neuronal development. Emerging evidence suggests that a subset of semaphorins, called 'immune semaphorins', function in the immune system. The class IV semaphorins Sema4D/CD100 and Sem...

journal_title：Journal of cell science

authors： Kumanogoh A,Kikutani H

更新日期：2003-09-01 00:00:00

abstract：:The non-alpha-helical N-terminal domain of intermediate filament proteins plays a key role in filament assembly. Previous studies have identified a nonapeptide motif, SSYRRIFGG, in the non-alpha-helical N-terminal domain of vimentin that is required for assembly. This motif is also found in desmin, peripherin and the ...

journal_title：Journal of cell science

authors： Ralton JE,Lu X,Hutcheson AM,Quinlan RA

更新日期：1994-07-01 00:00:00

abstract：:Many pathways dysregulated in prostate cancer are also involved in epithelial differentiation. To better understand prostate tumor initiation, we sought to investigate specific genes and mechanisms required for normal basal to luminal cell differentiation. Utilizing human prostate basal epithelial cells and an in vitr...

journal_title：Journal of cell science

authors： Frank SB,Berger PL,Ljungman M,Miranti CK

更新日期：2017-06-01 00:00:00

abstract：:Recent studies show that mitochondria and actin filaments work together in two contexts: (1) increased cytoplasmic calcium induces cytoplasmic actin polymerization that stimulates mitochondrial fission and (2) mitochondrial depolarization causes actin assembly around mitochondria, with roles in mitophagy. It is unclea...

journal_title：Journal of cell science

authors： Fung TS,Ji WK,Higgs HN,Chakrabarti R

更新日期：2019-09-23 00:00:00

abstract：:Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...

journal_title：Journal of cell science

authors： Abdelbaki A,Akman HB,Poteau M,Grant R,Gavet O,Guarguaglini G,Lindon C

更新日期：2020-06-16 00:00:00

abstract：:The nature, kinetics and cofactor requirements of leukocyte adhesion to cultured vascular cells have been investigated in vitro, using a model system in which leukocyte suspensions are in continuous motion over the cultured cells. Adhesion is assessed by histological examination or by using 51Cr-labelled leukocytes. L...

journal_title：Journal of cell science

authors： Beesley JE,Pearson JD,Carleton JS,Hutchings A,Gordon JL

更新日期：1978-10-01 00:00:00

abstract：:The insulin receptor tyrosine kinase substrate p53 (IRSp53) links Rac and WAVE2 and has been implicated in lamellipodia protrusion. Recently, however, IRSp53 has been reported to bind to both Cdc42 and Mena to induce filopodia. To shed independent light on IRSp53 function we determined the localisations and dynamics o...

journal_title：Journal of cell science

authors： Nakagawa H,Miki H,Nozumi M,Takenawa T,Miyamoto S,Wehland J,Small JV

更新日期：2003-06-15 00:00:00

abstract：:BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is ...

journal_title：Journal of cell science

authors： Ziv O,Finkelstein R,Suissa Y,Dinur T,Deshpande G,Gerlitz O

更新日期：2012-12-01 00:00:00

abstract：:Our previous studies of PAWS1 (protein associated with SMAD1; also known as FAM83G) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1-knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the ...

journal_title：Journal of cell science

authors： Cummins TD,Wu KZL,Bozatzi P,Dingwell KS,Macartney TJ,Wood NT,Varghese J,Gourlay R,Campbell DG,Prescott A,Griffis E,Smith JC,Sapkota GP

更新日期：2018-01-10 00:00:00

abstract：:In many physiological contexts, intracellular reduction-oxidation (redox) conditions and the unfolded protein response (UPR) are important for the control of cell life and death decisions. UPR is triggered by the disruption of endoplasmic reticulum (ER) homeostasis, also known as ER stress. Depending on the duration a...

journal_title：Journal of cell science

authors： Eletto D,Chevet E,Argon Y,Appenzeller-Herzog C

更新日期：2014-09-01 00:00:00

abstract：:There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...

journal_title：Journal of cell science

authors： Murate M,Abe M,Kasahara K,Iwabuchi K,Umeda M,Kobayashi T

更新日期：2015-04-15 00:00:00

abstract：:Monoclonal mouse hybridoma antibodies were obtained for secreted cellular fibronectin (cFn) from A8387 fibrosarcoma cells. One of them, 52-DH1 (DH), reacted exclusively with cFns but not with plasma Fns (pFns) in immunoblotting and solid-phase EIA. The DH antibody also recognized thermolysin cFn fragments and beta-gal...

journal_title：Journal of cell science

authors： Vartio T,Laitinen L,Närvänen O,Cutolo M,Thornell LE,Zardi L,Virtanen I

更新日期：1987-11-01 00:00:00

abstract：:Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...

journal_title：Journal of cell science

authors： McCaig CD

更新日期：1990-04-01 00:00:00

abstract：:We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apo...

journal_title：Journal of cell science

authors： Khan S,Kayahara M,Joashi U,Mazarakis ND,Sarraf C,Edwards AD,Hughes MN,Mehmet H

更新日期：1997-09-01 00:00:00

abstract：:Myofibrillar proteins assemble to form the highly ordered repetitive contractile structural unit known as a sarcomere. Studies of myogenesis in vertebrate cell culture and embryonic developmental systems have identified some of the processes involved during sarcomere formation. However, isoform changes during vertebra...

journal_title：Journal of cell science

authors： Nongthomba U,Clark S,Cummins M,Ansari M,Stark M,Sparrow JC

更新日期：2004-04-01 00:00:00

abstract：:The Dlg tumour suppressor protein is intimately involved in the control of cell contact and polarity. Previous studies have shown that hDlg is a target for a number of viral transforming proteins. In particular, the high risk human papillomavirus (HPV) E6 proteins target hDlg for proteasome-mediated degradation, an ac...

journal_title：Journal of cell science

authors： Mantovani F,Massimi P,Banks L

更新日期：2001-12-01 00:00:00

abstract：:In plant cells nucleolar fibrillar centres (FCs) undergo ultrastructural changes, depending on the nucleolar activity. We have found two types of FC structure in nucleoli with either high or low activity, to which we have given the conventional names of homogeneous and heterogeneous, respectively. The first type is ch...

journal_title：Journal of cell science

authors： Risueño MC,Medina FJ,Moreno Díaz de la Espina S

更新日期：1982-12-01 00:00:00

abstract：:An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...

journal_title：Journal of cell science

authors： Hearn PR,Russell RG,Farmer J

更新日期：1981-02-01 00:00:00

abstract：:In skeletal muscle, the triad is a structure comprising a transverse (T)-tubule and sarcoplasmic reticulum (SR) cisternae. Triads constitute the basis of excitation-contraction coupling as the cradle of the Ca2+ release complex. We have shown previously that triadin, a member of this complex, has shaping properties on...

journal_title：Journal of cell science

authors： Osseni A,Sébastien M,Sarrault O,Baudet M,Couté Y,Fauré J,Fourest-Lieuvin A,Marty I

更新日期：2016-10-15 00:00:00