Abstract：
:We report a fast method for the isolation of homogeneous C-protein from bovine skeletal muscle. In electron micrographs C-protein appears as short rods with a relatively uniform length of about 50 nm. Protein sequencing shows a single N-terminal sequence. Radio-labelled C-protein strongly decorates titin II and myosin rods but not myosin heads. Binding to titin II is retained in preparations lacking titin-associated proteins. Antibodies to bovine C-protein were used to screen a lambda gt11 cDNA library constructed from fetal human skeletal muscle. Clone HC38 is 3833 bp long and encodes a protein of 1138 amino acid residues. The start of the predicted sequence fits the N-terminal sequence of the bovine protein. All partial sequences obtained from the bovine protein (348 residues) and the sequence deduced from a partial chicken cDNA (Einheber and Fischman, 1990) can be aligned along the human sequence. The sequences of human and chicken C-proteins share 50% identity and 70% similarity. Along the repeat patterns of the human protein the fibronectin (Fn)-like domains are better conserved than the immunoglobulin (Ig)-like domains. Regions of strong divergence between chicken fast C-protein and human slow C-protein may represent differences in C-protein isoforms.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Fürst DO,Vinkemeier U,Weber Kkeywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
769-78eissn
0021-9533issn
1477-9137journal_volume
102 ( Pt 4)pub_type
杂志文章abstract：:Here we present experimental evidence that phagocytic cells use modulation of specific components of their glycocalyx to regulate their binding capacity. Particles coated with antibodies specific for the CD32 medium affinity IgG receptor were driven along human monocytic THP-1 cells (expressing CD32) in a flow chamber...
journal_title：Journal of cell science
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doi：
更新日期：1994-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1980-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1992-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of cell science
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更新日期：1992-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-08-15 00:00:00
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