Trafficking and signaling through the cytoskeleton: a specific mechanism.

abstract：:Keratins 8 and 18 (collectively referred to as K8/K18) are the major components of intermediate filaments of simple epithelial cells. Recent studies have revealed the function of K8/K18 in apoptosis modulation. Here, we show that eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation...

journal_title：Journal of cell science

authors： Lin YM,Chen YR,Lin JR,Wang WJ,Inoko A,Inagaki M,Wu YC,Chen RH

更新日期：2008-07-15 00:00:00

abstract：:In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NP...

journal_title：Journal of cell science

authors： Umlauf D,Bonnet J,Waharte F,Fournier M,Stierle M,Fischer B,Brino L,Devys D,Tora L

更新日期：2013-06-15 00:00:00

abstract：:The coordinated cellular responses to physiological stress are known to be effected in part by the activation of heat-shock factor 1, a transcriptional activator protein capable of binding to, and inducing transcription from genes containing heat shock elements. Other stress responsive signal transduction pathways als...

journal_title：Journal of cell science

authors： Head MW,Hurwitz L,Goldman JE

更新日期：1996-05-01 00:00:00

abstract：:Here, we identify a role for the matrilin-2 (Matn2) extracellular matrix protein in controlling the early stages of myogenic differentiation. We observed Matn2 deposition around proliferating, differentiating and fusing myoblasts in culture and during muscle regeneration in vivo. Silencing of Matn2 delayed the express...

journal_title：Journal of cell science

authors： Deák F,Mátés L,Korpos E,Zvara A,Szénási T,Kiricsi M,Mendler L,Keller-Pintér A,Ozsvári B,Juhász H,Sorokin L,Dux L,Mermod N,Puskás LG,Kiss I

更新日期：2014-08-01 00:00:00

abstract：:In the developing Drosophila eye, the centrioles of the differentiating retinal cells are not surrounded by the microtubule-nucleating γ-tubulin, suggesting that they are unable to organize functional microtubule-organizing centers. Consistent with this idea, Cnn and Spd-2, which are involved in γ-tubulin recruitment,...

journal_title：Journal of cell science

authors： Riparbelli MG,Persico V,Gottardo M,Callaini G

更新日期：2018-02-22 00:00:00

abstract：:We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quanti...

journal_title：Journal of cell science

authors： Lourim D,Kempf A,Krohne G

更新日期：1996-07-01 00:00:00

abstract：:MIP and MP70 are putative gap junction components in the plasma membranes of the mammalian lens fibre cells. We show now that MP70 can be solubilized separately from MIP in mild detergent solutions, and that this treatment results in the dissociation of the fibre gap junctions. Solubilized MP70 was isolated as 16.9 S ...

journal_title：Journal of cell science

authors： Kistler J,Bullivant S

更新日期：1988-11-01 00:00:00

abstract：:The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. Nuclear proteins that are localized in discrete domains within the nucleus include the leukemia-associated acute myelogenous leukemia (AML) an...

journal_title：Journal of cell science

authors： Stein GS,van Wijnen AJ,Stein JL,Lian JB,Montecino M,Choi J,Zaidi K,Javed A

更新日期：2000-07-01 00:00:00

abstract：:Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed and stabilized by E-cadherin and catenin proteins linked to the actomyosin cyt...

journal_title：Journal of cell science

authors： Le Bras S,Le Borgne R

更新日期：2014-12-15 00:00:00

abstract：:The roles of P21-activated kinase (PAK) in the regulation of axon outgrowth downstream of extracellular matrix (ECM) proteins are poorly understood. Here we show that PAK1-3 and PIX are expressed in the developing spinal cord and differentially localize to point contacts and filopodial tips within motile growth cones....

journal_title：Journal of cell science

authors： Santiago-Medina M,Gregus KA,Gomez TM

更新日期：2013-03-01 00:00:00

abstract：:The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here, we show that staurosporine causes defined changes in cytosolic free Ca(2+) ([Ca(2+)]c) dynamics and a distinct Ca(2+) signature that involves Ca(2+) influx from the external medium and internal Ca(2+) stores. We i...

journal_title：Journal of cell science

authors： Gonçalves AP,Cordeiro JM,Monteiro J,Muñoz A,Correia-de-Sá P,Read ND,Videira A

更新日期：2014-09-01 00:00:00

abstract：:The core protease (CP) subcomplex of the 26S proteasome houses the proteolytic active sites and assumes a barrel shape comprised of four co-axially stacked heptameric rings formed by structurally related α- and β-subunits. CP biogenesis typically begins with the assembly of the α-ring, which then provides a template f...

journal_title：Journal of cell science

authors： Marshall RS,Gemperline DC,McLoughlin F,Book AJ,Hofmann K,Vierstra RD

更新日期：2020-11-03 00:00:00

abstract：:When they are fixed with glutaraldehyde in the presence of calcium ions, the plasma membranes of Beroe giant smooth muscle fibres display micropapillae filled with an electron-dense deposit. After freeze-fracturing of fixed tissue, the micropapillae are still present, therefore their shape and size are determined duri...

journal_title：Journal of cell science

authors： Nicaise G,Hernandez-Nicaise ML,Malaval L

更新日期：1982-06-01 00:00:00

abstract：:The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a hig...

journal_title：Journal of cell science

authors： Helmke S,Lohse K,Mikule K,Wood MR,Pfenninger KH

更新日期：1998-08-01 00:00:00

abstract：:Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...

journal_title：Journal of cell science

authors： Dawe HR,Adams M,Wheway G,Szymanska K,Logan CV,Noegel AA,Gull K,Johnson CA

更新日期：2009-08-01 00:00:00

abstract：:During angiogenesis, endothelial cells must coordinate matrix proteolysis with migration. Here, we tested whether the focal adhesion scaffold protein Hic-5 (also known as TGFB1I1) regulated endothelial sprouting in three dimensions. Hic-5 silencing reduced endothelial sprouting and lumen formation, and sprouting defec...

journal_title：Journal of cell science

authors： Dave JM,Abbey CA,Duran CL,Seo H,Johnson GA,Bayless KJ

更新日期：2016-02-15 00:00:00

abstract：:In chondrocytes, PTHrP maintains them in a proliferative state and prevents premature hypertrophy. The mechanism by which PTHrP does this is not fully understood. Both Runx2 and Runx3 are required for chondrocyte maturation. We recently demonstrated that cyclin D1 induces Runx2 protein phosphorylation and degradation....

journal_title：Journal of cell science

authors： Zhang M,Xie R,Hou W,Wang B,Shen R,Wang X,Wang Q,Zhu T,Jonason JH,Chen D

更新日期：2009-05-01 00:00:00

abstract：:Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) act as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of th...

journal_title：Journal of cell science

authors： Lozano-Elena F,Planas-Riverola A,Vilarrasa-Blasi J,Schwab R,Caño-Delgado AI

更新日期：2018-01-29 00:00:00

abstract：:Embryonal rhabdomyosarcomas (ERMSs) show elevated levels of PAX7, a transcription factor that marks quiescent adult muscle stem (satellite) cells and is important for proliferation and survival of activated satellite cells and whose timely repression is required for myogenic differentiation. However, the mechanism of ...

journal_title：Journal of cell science

authors： Riuzzi F,Sorci G,Sagheddu R,Sidoni A,Alaggio R,Ninfo V,Donato R

更新日期：2014-04-15 00:00:00

abstract：:Human breast cancer cell lines, as well as transformed mammary epithelial cells (HBL-100) and growth-stimulated normal breast epithelial cells showed positive cytochemical reaction with the proteinase substrate 2-(N-benzyloxycarbonyl-L-arginyl-L-arginylamido)-4-methoxynapht halene, in the presence of 5-nitrosalicylald...

journal_title：Journal of cell science

authors： Krepela E,Bártek J,Skalková D,Vicar J,Rasnick D,Taylor-Papadimitriou J,Hallowes RC

更新日期：1987-02-01 00:00:00

abstract：:The main pathology of cystic fibrosis results from obstruction of ducts in several organs by mucous secretions. The cause of this obstruction remains unclear. We have examined expression of the cystic fibrosis transmembrane conductance regulator (CFTR) and of the major pancreatic mucin, MUC1, in primary pancreatic duc...

journal_title：Journal of cell science

authors： Chambers JA,Harris A

更新日期：1993-06-01 00:00:00

abstract：:Naegleria gruberi is a single-celled eukaryote best known for its remarkable ability to form an entire microtubule cytoskeleton de novo during its metamorphosis from an amoeba into a flagellate, including basal bodies (equivalent to centrioles), flagella and a cytoplasmic microtubule array. Our publicly available full...

journal_title：Journal of cell science

authors： Fritz-Laylin LK,Cande WZ

更新日期：2010-12-01 00:00:00

abstract：:Understanding the interactions between distinct epithelial cells would help us to understand the development of tissues. Drosophila imaginal discs, which are made up of two types of epithelial cells, provide good model systems for such studies. The disc proper or the columnar epithelial cells are apposed to a layer of...

journal_title：Journal of cell science

authors： Pallavi SK,Shashidhara LS

更新日期：2005-08-01 00:00:00

abstract：:By differential screening of a Xenopus laevis egg cDNA library, we have isolated a 2,111 bp cDNA which corresponds to a maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes a 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members of a new protein...

journal_title：Journal of cell science

authors： Roghi C,Giet R,Uzbekov R,Morin N,Chartrain I,Le Guellec R,Couturier A,Dorée M,Philippe M,Prigent C

更新日期：1998-03-01 00:00:00

abstract：:Class-V myosins are two-headed actin-based mechanoenzymes that function in the transport and subcellular localization of organelles and possibly in the outgrowth of cellular processes. To determine which domains of myosin-V are involved in intracellular localization of this motor protein, we have expressed fusions of ...

journal_title：Journal of cell science

authors： Tsakraklides V,Krogh K,Wang L,Bizario JC,Larson RE,Espreafico EM,Wolenski JS

更新日期：1999-09-01 00:00:00

abstract：:The insulin receptor tyrosine kinase substrate p53 (IRSp53) links Rac and WAVE2 and has been implicated in lamellipodia protrusion. Recently, however, IRSp53 has been reported to bind to both Cdc42 and Mena to induce filopodia. To shed independent light on IRSp53 function we determined the localisations and dynamics o...

journal_title：Journal of cell science

authors： Nakagawa H,Miki H,Nozumi M,Takenawa T,Miyamoto S,Wehland J,Small JV

更新日期：2003-06-15 00:00:00

abstract：:Human cytomegalovirus (HCMV) establishes life-long latent infection in hematopoietic progenitor cells and circulating monocytes in infected individuals. Myeloid differentiation coupled with immune dysregulation leads to viral reactivation, which can cause severe disease and mortality. Reactivation of latent virus requ...

journal_title：Journal of cell science

authors： Krishna BA,Wass AB,Dooley AL,O'Connor CM

更新日期：2021-01-25 00:00:00

abstract：:Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, the underlying mechanisms are still poorly understood. To address this issue, we assessed the effect of fibroblast-derived diffusible factors on the growth and morph...

journal_title：Journal of cell science

authors： Soriano JV,Pepper MS,Nakamura T,Orci L,Montesano R

更新日期：1995-02-01 00:00:00

abstract：:Many pathways dysregulated in prostate cancer are also involved in epithelial differentiation. To better understand prostate tumor initiation, we sought to investigate specific genes and mechanisms required for normal basal to luminal cell differentiation. Utilizing human prostate basal epithelial cells and an in vitr...

journal_title：Journal of cell science

authors： Frank SB,Berger PL,Ljungman M,Miranti CK

更新日期：2017-06-01 00:00:00

abstract：:CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and ...

journal_title：Journal of cell science

authors： Otake K,Ohzeki JI,Shono N,Kugou K,Okazaki K,Nagase T,Yamakawa H,Kouprina N,Larionov V,Kimura H,Earnshaw WC,Masumoto H

更新日期：2020-08-11 00:00:00