Abstract:
:Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, the underlying mechanisms are still poorly understood. To address this issue, we assessed the effect of fibroblast-derived diffusible factors on the growth and morphogenetic properties of a clonally derived subpopulation (clone TAC-2) of normal murine mammary gland (NMuMG) epithelial cells embedded in collagen gels. Under control conditions, TAC-2 mammary gland epithelial cells suspended within collagen gels formed either irregularly shaped cell aggregates or short branching cord-like structures. Addition of conditioned medium from Swiss 3T3 or MRC-5 fibroblasts dramatically stimulated cord formation by TAC-2 cells, resulting in the development of an extensive, highly arborized system of duct-like structures, which in appropriate sections were seen to contain a central lumen. The effect of fibroblast conditioned medium was completely abrogated by antibodies against hepatocyte growth factor (also known as scatter factor), a fibroblast-derived polypeptide that we have previously shown induces tubulogenesis by Madin-Darby canine kidney epithelial cells. Addition of exogenous recombinant human hepatocyte growth factor to collagen gel cultures of TAC-2 cells mimicked the tubulogenic activity of fibroblast conditioned medium by stimulating formation of branching duct-like structures in a dose-dependent manner, with a maximal 77-fold increase in cord length at 20 ng/ml. The effect of either fibroblast conditioned medium or hepatocyte growth factor was markedly potentiated by the simultaneous addition of hydrocortisone (1 microgram/ml), which also enhanced lumen formation. These results demonstrate that hepatocyte growth factor promotes the formation of branching duct-like structures by mammary gland epithelial cells in vitro, and suggest that it may act as a mediator of the inducing effect of mesenchyme (or stroma) on mammary gland development.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Soriano JV,Pepper MS,Nakamura T,Orci L,Montesano Rsubject
Has Abstractpub_date
1995-02-01 00:00:00pages
413-30eissn
0021-9533issn
1477-9137journal_volume
108 ( Pt 2)pub_type
杂志文章abstract::It has been demonstrated that proteins covalently conjugated to folic acid may be taken up by cells via endocytosis after binding to a folate binding protein (FBP) in the cell membrane. The proteins taken up in this manner remain catalytically active and they may modify physiological processes occurring in the cytosol...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Purified nuclei retaining a high degree of ultrastructural integrity were isolated by conventional centrifugation techniques. The cytoplasmic surface of these nuclei was iodinated using lactoperoxidase immobilized onto giant Sepharose beads; thus the outer nuclear membrane and the cytoplasmic surface of nuclear pore c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::To examine the role of the keratin head region and its subdomains in filament assembly we constructed several deletion mutants of type I and type II keratins and analysed their in vitro IF forming capacity. The delta K8 (1-74) and delta K18 (1-56), mutants formed only soluble oligomers, predominantly tetramers with th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::Planar cell polarity (PCP) has been demonstrated in the epithelium of organisms from flies to humans. Recent research has revealed that the planar organization of cells requires a conserved set of genes, known as the PCP genes. Tbe PCP proteins Frizzled (Fz) and Dishevelled (Dsh) function as key players in PCP signall...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.00973
更新日期:2004-02-01 00:00:00
abstract::The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091066
更新日期:2012-03-15 00:00:00
abstract::The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Developing oral organelles of dividing Tetrahymena corlissi appear to be positioned by mechanisms which assess distances as a proportion of the organism's overall dimensions. In some respects, the cortex of this protozoan obeys the 'French flag' rule formulated by Wolpert for describing regulation of spatial proportio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::The Arp2/3 complex is important for morphogenesis in various developmental systems, but specific in vivo roles for this complex in cells that move during morphogenesis are not well understood. We have examined cellular roles for Arp2/3 in the Caenorhabditis elegans embryo. In C. elegans, the first morphogenetic moveme...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.057562
更新日期:2009-11-01 00:00:00
abstract::Inhibitory and excitatory neurons exhibit distinct patterns of development in the mammalian cerebral cortex. The morphological development of inhibitory and excitatory neurons derived from fetal rat cerebral cortex has now been compared in vitro. Inhibitory neurons were identified by immunofluorescence staining with a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00762
更新日期:2003-11-01 00:00:00
abstract::Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0-5-I mum and mounted in Farrants' gum medium containing ethyl gallate, show good staining of lipid-contaning structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microsco...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-12-01 00:00:00
abstract::In plant cells nucleolar fibrillar centres (FCs) undergo ultrastructural changes, depending on the nucleolar activity. We have found two types of FC structure in nucleoli with either high or low activity, to which we have given the conventional names of homogeneous and heterogeneous, respectively. The first type is ch...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::Fluorescence in situ hybridization has demonstrated that chromosomes form individual territories in interphase nuclei. However, this technique is not suitable to determine whether territories are mutually exclusive or interwoven. This notion, however, is essential for understanding functional organizations in the cell...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Sea urchin coelomocytes undergo an inducible structural transformation from petalloid to filopodial form during the 'clotting' response in sea urchins. Using a petalloid coelomocyte model, stimulated coelomocytes exhibited bidirectional particle/vesicle motility with a broad distribution of velocities, ranging from 0....
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::We have explored the biological function of a surface glycoprotein (GP72) of Trypanosoma cruzi by studying a null mutant parasite, generated by targeted gene deletion. GP72 deletion affected parasite morphology in several stages of the life cycle. Insect midgut (epimastigote) forms had a detached flagellum (apomastigo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Tenascin, an extracellular matrix protein that modulates cell adhesion, exists as a unique six-armed structure called a hexabrachion. The human hexabrachion is composed of six identical 320 kDa subunits and the structure is stabilized by inter-subunit disulfide bonds between amino-terminal segments. We have examined t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Alpha-satellite (alphoid) DNA is necessary for de novo formation of human artificial chromosomes (HACs) in human cultured cells. To investigate the relationship among centromeric, transcriptionally permissive and non-permissive chromatin assemblies on de novo HAC formation, we constructed bacterial artificial chromoso...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02702
更新日期:2005-12-15 00:00:00
abstract::Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.192781
更新日期:2017-07-15 00:00:00
abstract::A fundamental aspect in the morphogenesis of a polarized epithelium is the formation of structurally and functionally distinct apical and basal-lateral domains of the plasma membrane. The formation of these membrane domains involves the accumulation of domain-specific proteins and removal of incorrectly localized prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::S100 proteins have attracted great interest in recent years because of their cell- and tissue-specific expression and association with various human pathologies. Most S100 proteins are small acidic proteins with calcium-binding domains - the EF hands. It is thought that this group of proteins carry out their cellular ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract::Crumbs (Crb) is an evolutionarily conserved transmembrane protein localised to the apical membrane of epithelial cells. Loss or mislocalisation of Crb is often associated with disruption of apicobasal cell polarity. crb mRNA is also apically enriched in epithelial cells, and, as shown here, accumulates in the oocyte o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.236497
更新日期:2021-01-25 00:00:00
abstract::The novel protein Nowa was identified in nematocysts, explosive organelles of Hydra, jellyfish, corals and other CNIDARIA: Biogenesis of these organelles is complex and involves assembly of proteins inside a post-Golgi vesicle to form a double-layered capsule with a long tubule. Nowa is the major component of the oute...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00084
更新日期:2002-10-15 00:00:00
abstract::The essential gene noa (CG 3971; also known as Baldspot) encodes a very long chain fatty acid elongase which is most similar to the mammalian elongase ELOVL6. noa is expressed in the nervous system from embryogenesis on, in imaginal discs, the fat body, malpighian tubules and in the gonads of both sexes. Its function ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.006551
更新日期:2007-08-15 00:00:00
abstract::The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with human cancer, small GTPases and their regulatory proteins have become increasingly attractive for the development of novel therapeutics. Class...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.210419
更新日期:2018-01-08 00:00:00
abstract::Oncogenic forms of the non-receptor tyrosine kinase Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that they do so by influencing the RhoA-ROCK pathway t...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.01111
更新日期:2004-03-01 00:00:00
abstract::The effects of isolated plasma membranes on cohesion of Dictyostelium discoideum Ax-2 cells have been studied. Membranes isolated from cells in the log phase of growth gave complete inhibition of log-phase cell cohesion. This effect was specific for the cohesion log-phase cells mediated by contact sites B, since the c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::The constitutive recirculation of lymphocytes between the widely distributed organs of the immune system is essential for host defence. We have developed an in vitro model of lymphocyte migration from the blood into lymph nodes which employs primary cultures of high endothelial cells (HEC). HEC-adherent lymphocytes ad...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::Rothmund-Thomson syndrome (RTS) is a human genetic disorder characterized by genome instability, cancer susceptibility and premature aging. The gene defective in a subset of RTS cases, RECQL4, encodes a member of the RecQ family of DNA helicases. To better define the function of the RECQL4 protein, we have determined ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02556
更新日期:2005-09-15 00:00:00
abstract::Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.030379
更新日期:2008-07-01 00:00:00