Abstract：
:Alpha-satellite (alphoid) DNA is necessary for de novo formation of human artificial chromosomes (HACs) in human cultured cells. To investigate the relationship among centromeric, transcriptionally permissive and non-permissive chromatin assemblies on de novo HAC formation, we constructed bacterial artificial chromosome (BAC)-based linear HAC vectors whose left vector arms are occupied by beta geo coding genes with or without a functional promoter in addition to a common marker gene on the right arm. Although HACs were successfully generated from the vectors with promoter-less constructs on the left arm in HT1080 cells, we failed to generate a stable HAC from the vectors with a functional promoter on the left arm. Despite this failure in HAC formation, centromere components (CENP-A, CENP-B and CENP-C) assembled at the integration sites correlating with a transcriptionally active state of both marker genes on the vector arms. However, on the stable HAC, chromatin immunoprecipitation analysis showed that HP1alpha and trimethyl histone H3-K9 were enriched at the non-transcribing left vector arm. A transcriptionally active state on both vector arms is not compatible with heterochromatin formation on the introduced BAC DNA, suggesting that epigenetic assembly of heterochromatin is distinct from centromere chromatin assembly and is required for the establishment of a stable artificial chromosome.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Nakashima H,Nakano M,Ohnishi R,Hiraoka Y,Kaneda Y,Sugino A,Masumoto Hdoi
10.1242/jcs.02702keywords:
subject
Has Abstractpub_date
2005-12-15 00:00:00pages
5885-98issue
Pt 24eissn
0021-9533issn
1477-9137pii
118/24/5885journal_volume
118pub_type
杂志文章abstract：:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and hande...
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doi：10.1242/jcs.116996
更新日期：2013-03-15 00:00:00
abstract：:The glucocorticoid receptor and the mineralocorticoid receptor are hormone-dependent transcription factors. They regulate the excitability of rat hippocampus CA1 neurons in a coordinated fashion. We studied the spatial distribution of these transcription factors in nuclei of CA1 neurons by dual labeling immunocytochem...
journal_title：Journal of cell science
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abstract：:The cellular microenvironment, characterized by an extracellular matrix (ECM), played an essential role in the transition from unicellularity to multicellularity in animals (metazoans), and in the subsequent evolution of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily -...
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abstract：:The karyo-cytoskeleton of cells cultured in vitro was investigated employing resinless section immunogold electron microscopy. Cells were entrapped in low-melting agarose, sequentially extracted with various buffers and digested with nucleases to obtain karyo-cytoskeletal frameworks and reacted with specific primary a...
journal_title：Journal of cell science
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journal_title：Journal of cell science
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abstract：:Iron (Fe(2+), Fe(3+)) homeostasis is a tightly regulated process, involving precise control of iron influx and egress from cells. Although the mechanisms of its import into cells by iron carrier molecules are well characterized, iron export remains poorly understood. The current paradigm envisages unique functions ass...
journal_title：Journal of cell science
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更新日期：2014-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2013-09-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1999-07-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2003-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.023150
更新日期：2008-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.02951
更新日期：2006-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1987-03-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1999-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1995-02-01 00:00:00
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更新日期：1979-08-01 00:00:00
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更新日期：1977-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1975-06-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1979-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1998-01-01 00:00:00
