Abstract：
:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and handed over to the Sar1 and COPII machinery in ERES, and a capture model, where cargo freely diffuses into ERES where it is captured by the Sar1 and COPII machinery. Using the novel secretion inhibitor FLI-06, we show that recruitment of the cargo VSVG to ERES is an active process upstream of Sar1 and COPII. Applying FLI-06 before concentration of VSVG in ERES completely abolishes its recruitment. In contrast, applying FLI-06 after VSVG concentration in ERES does not lead to dispersal of the concentrated VSVG, arguing that it inhibits recruitment to ERES as opposed to capture in ERES. FLI-06 also inhibits export out of the trans-Golgi network (TGN), suggesting that similar mechanisms might orchestrate cargo selection and concentration at the ER and TGN. FLI-06 does not inhibit autophagosome biogenesis and the ER-peroxisomal transport route, suggesting that these rely on different mechanisms.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Yonemura Y,Li X,Müller K,Krämer A,Atigbire P,Mentrup T,Feuerhake T,Kroll T,Shomron O,Nohl R,Arndt HD,Hoischen C,Hemmerich P,Hirschberg K,Kaether Cdoi
10.1242/jcs.186163subject
Has Abstractpub_date
2016-10-15 00:00:00pages
3868-3877issue
20eissn
0021-9533issn
1477-9137pii
jcs.186163journal_volume
129pub_type
杂志文章abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.067512
更新日期：2010-08-01 00:00:00
abstract：:Endothelial cells (ECs) sense shear stress and transduce blood flow information into functional responses that play important roles in vascular homeostasis and pathophysiology. A unique feature of shear-stress-sensing is the involvement of many different types of membrane-bound molecules, including receptors, ion chan...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.119628
更新日期：2013-03-01 00:00:00
abstract：:Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.064022
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abstract：:Although many pathogenic copy number variations (CNVs) are associated with neuropsychiatric diseases, few of them have been functionally characterised. Here we report multiple schizophrenia cases with CNV abnormalities specific to unc-51-like kinase 4 (ULK4), a serine/threonine kinase gene. Deletions spanning exons 21...
journal_title：Journal of cell science
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doi：10.1242/jcs.137604
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
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abstract：:Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:The role of the haemolytic activity of Sendai virus in cell-cell fusion has been examined in monolayers of human erythrocytes and erythrocyte ghosts fused with either haemolytic or non-haemolytic virus. Morphological observations indicate that cell swelling and haemolysis is a distinct event in cell-cell fusion irresp...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.105981
更新日期：2012-09-15 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：
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doi：
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journal_title：Journal of cell science
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更新日期：1979-06-01 00:00:00
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journal_title：Journal of cell science
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doi：
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