Abstract:
:In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA and RNA in such a preparation because the presence of the RNA component causes resistance of DNA to DNase I and Exo III. An intimate, hybrid-type association of part of the RNA component with DNA is indicated also by the fact that about 20% of this RNA is sensitive to RNase H. By specific inhibition of the RNA synthesis with alpha-amanitin and actinomycin D it was shown that the bulk of associated RNA is transcribed by RNA polymerase III. Hybridization experiments showed similarity between the DNA sequences isolated from the complexes and those from the base of dehistonized DNA loops obtained by high-salt extraction of nuclei. This observation suggests that the complexes might represent attachment sites of nuclear DNA to the matrix: possibly, the attachment is mediated via the RNA component. Experiments with induction of erythroid differentiation indicated that a profound reorganization of the nucleus, accompanying terminal differentiation, leads to a striking reduction in the number of complexes and thus in the number of attachment sites. This suggests that the complexes should function as transient attachment sites.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Patriotis C,Andreeva M,Pascaleva M,Ivanov V,Djondjurov Lsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
667-74eissn
0021-9533issn
1477-9137journal_volume
95 ( Pt 4)pub_type
杂志文章abstract::Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01333
更新日期:2004-09-15 00:00:00
abstract::The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.148296
更新日期:2015-05-15 00:00:00
abstract::Cornichon proteins are structurally related transmembrane proteins that have been studied in and Drosophila and yeast. In Drosophila, Cornichon (Cni) is involved in embryo polarization by the TGFalpha-related Gurken. In yeast, the Cni-related Erv14 is required for axial budding. A cargo receptor function has been prop...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.004200
更新日期:2007-07-15 00:00:00
abstract::Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.105163
更新日期:2012-11-15 00:00:00
abstract::Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03455
更新日期:2007-06-01 00:00:00
abstract::Cells depend on a highly ordered organisation of their content and must develop strategies to maintain the anisotropic distribution of organelles during periods of nutrient shortage. One of these strategies is to solidify the cytoplasm, which was observed in bacteria and yeast cells with acutely interrupted energy pro...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.231688
更新日期:2019-11-08 00:00:00
abstract::Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216093
更新日期:2018-08-13 00:00:00
abstract::In the central nervous system, oligodendrocytes synthesize vast amounts of myelin, a multilamellar membrane wrapped around axons that dramatically enhances nerve transmission. A complex apparatus appears to coordinate trafficking of proteins and lipids during myelin synthesis, but the molecular interactions involved a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02785
更新日期:2006-03-01 00:00:00
abstract::Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::Increased deposition of collagen in extracellular matrix (ECM) leads to increased tissue stiffness and occurs in breast tumors. When present, this increases tumor invasion and metastasis. Precisely how this deposition is regulated and maintained in tumors is unclear. Much has been learnt about mechanical signal transd...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.180539
更新日期:2016-05-15 00:00:00
abstract::The neuron specific Drosophila ELAV protein belongs to the ELAV family of RNA binding proteins which are characterized by three highly conserved RNA recognition motifs, an N-terminal domain, and a hinge region between the second and third RNA recognition motifs. Despite their highly conserved RNA recognition motifs th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Mitosis involves considerable membrane remodelling and vesicular trafficking to generate two independent cells. Consequently, endocytosis and endocytic proteins are required for efficient mitotic progression and completion. Several endocytic proteins also participate in mitosis in an endocytosis-independent manner. He...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.105981
更新日期:2012-09-15 00:00:00
abstract::Junction dynamics of endothelial cells are based on the integration of signal transduction, cytoskeletal remodeling and contraction, which are necessary for the formation and maintenance of monolayer integrity, but also enable repair and regeneration. The VE-cadherin-catenin complex forms the molecular basis of the ad...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.222893
更新日期:2019-01-03 00:00:00
abstract::As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its sy...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::A conjugate of horseradish peroxidase (HRP) to poly(L-lysine) (PLL) was used as a non-specific adsorptive probe to study transcytosis in MDCK strain I and Caco-2 epithelial cells. As we have shown previously, HRP-PLL transcytosis proceeds via an intracellular, non-lysosomal proteolytic compartment in MDCK cells; yet, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::The large GTPase dynamin, long known for its role in endocytosis, has most recently been implicated as a facilitator of cell migration and invasion. Recent observations link dynamin to the cycle of membrane expansion and retraction essential for cell motility. Its role in actin polymerization, membrane deformation and...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02963
更新日期:2006-05-01 00:00:00
abstract::The events that accompany sporogenesis in the apogamous fern Dryopteris borreri parallel those seen in sexually reproducing ferns. Organelles dedifferentiate and redifferentiate, and form a discrete band across the equator of dyads; nuclear vacuoles and lipid spherosomes appear during prophase, and the major part of t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The presence of high-ploidy cells in malignant tumours has long been documented. However, the biological significance of these cells is not known and there is a great deal of controversy over their proliferative potential. We have analysed the behaviour of these cells in two murine tumour lines, B16F10 melanoma and 3T...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Cytidine triphosphate synthase (CTPS) and inosine monophosphate dehydrogenase (IMPDH) (both of which have two isoforms) can form fiber-like subcellular structures termed 'cytoophidia' under certain circumstances in mammalian cells. Although it has been shown that filamentation of CTPS downregulates its activity by dis...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.175265
更新日期:2015-10-01 00:00:00
abstract::The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Stages of meiosis from the bluebell Endymion non-scriptus (L.) were studied by electron microscopy. The nucleolus went through the process of segregation at the beginning of meiosis with the movement to its surface of a pale-staining region. This region was shown to be the same as that called the 'L zone' or lacunae o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::In adipocytes and muscle cells, the GLUT4 glucose transporter isoform is present in intracellular vesicles which continuously recycle between an intracytoplasmic location and the plasma membrane. It is not clear whether the GLUT4-vesicles represent a specific kind of vesicle or resemble typical secretory granules or s...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Developing oral organelles of dividing Tetrahymena corlissi appear to be positioned by mechanisms which assess distances as a proportion of the organism's overall dimensions. In some respects, the cortex of this protozoan obeys the 'French flag' rule formulated by Wolpert for describing regulation of spatial proportio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Candida albicans is a dimorphic fungus capable of growing as a budding yeast and as a filamentous hypha. We have used the technique of immunofluorescence to study the changes in the microtubule cytoskeleton during the cell cycle in both growth forms. This approach has revealed the presence of an extensive system of mi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00