Proliferative behaviour of high-ploidy cells in two murine tumour lines.

abstract：:A cDNA encoding Xlamin B1 was cloned from a whole ovary mRNA by RT-PCR. GST-lamin fusion constructs were generated from this cDNA by first creating convenient restriction sites within the Xlamin B1 coding sequence, using PCR directed mutagenesis, and then sub-cloning relevant sequences into pGEX-4T-3. Two expression c...

journal_title：Journal of cell science

authors： Ellis DJ,Jenkins H,Whitfield WG,Hutchison CJ

更新日期：1997-10-01 00:00:00

abstract：:Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...

journal_title：Journal of cell science

authors： Dylewski DP,Keenan TW

更新日期：1984-12-01 00:00:00

abstract：:Abnormal cardiac valve morphogenesis is a common cause of human congenital heart disease. The molecular mechanisms regulating endocardial cell proliferation and differentiation into cardiac valves remain largely unknown, although great progress has been made on the endocardial contribution to the atrioventricular cush...

journal_title：Journal of cell science

authors： Wang X,Yu Q,Wu Q,Bu Y,Chang NN,Yan S,Zhou XH,Zhu X,Xiong JW

更新日期：2013-03-15 00:00:00

abstract：:The mammalian lens consists of an aged core of quiescent cells enveloped by a layer of synthetically active cells. Abundant gap junctions within and between these cell populations ensure that the lens functions as an electrical syncytium and facilitates the exchange of small molecules between surface and core cells. I...

journal_title：Journal of cell science

authors： Shestopalov VI,Bassnett S

更新日期：2003-10-15 00:00:00

abstract：:Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles. LETS protein was detected by...

journal_title：Journal of cell science

authors： Marshall CJ,Humphryes KC,Pollack RE

更新日期：1978-10-01 00:00:00

abstract：:The adaptor protein Miranda plays a pivotal role in the asymmetric cell division of neuroblasts by asymmetrically segregating key differentiation factors. Miranda localization requires Myosin VI and Myosin II. The apical-then-basal localization pattern of Miranda detected in fixed tissue, and the localization defects ...

journal_title：Journal of cell science

authors： Erben V,Waldhuber M,Langer D,Fetka I,Jansen RP,Petritsch C

更新日期：2008-05-01 00:00:00

abstract：:Cilia and flagella are eukaryotic organelles involved in multiple cellular functions. The primary cilium is generally non motile and found in numerous vertebrate cell types where it controls key signalling pathways. Despite a common architecture, ultrastructural data suggest some differences in their organisation. Her...

journal_title：Journal of cell science

authors： Molla-Herman A,Ghossoub R,Blisnick T,Meunier A,Serres C,Silbermann F,Emmerson C,Romeo K,Bourdoncle P,Schmitt A,Saunier S,Spassky N,Bastin P,Benmerah A

更新日期：2010-05-15 00:00:00

abstract：:Biglycan and decorin are small chondroitin/dermatan sulphate proteoglycans in the extracellular matrix of connective tissue that belong to the family of structurally related proteoglycans called small leucine-rich repeat proteins. We show for the first time that biglycan and decorin induce morphological and cytoskelet...

journal_title：Journal of cell science

authors： Tufvesson E,Westergren-Thorsson G

更新日期：2003-12-01 00:00:00

abstract：:The postnatal mouse ovary is rich in quiescent and early-growing oocytes, each one surrounded by a layer of somatic granulosa cells (GCs) on a basal lamina. As oocytes start to grow the GCs change shape from flattened to cuboidal, increase their proliferation and form multiple layers, providing a unique model for stud...

journal_title：Journal of cell science

authors： Da Silva-Buttkus P,Jayasooriya GS,Mora JM,Mobberley M,Ryder TA,Baithun M,Stark J,Franks S,Hardy K

更新日期：2008-12-01 00:00:00

abstract：:Human skin diseases have revealed fundamental mechanisms by which cytoskeletal proteins contribute to tissue architecture and function. In particular, the analysis of epidermal blistering disorders and the role of keratin gene mutations in these diseases has led to significant increases in our understanding of interme...

journal_title：Journal of cell science

authors： Kottke MD,Delva E,Kowalczyk AP

更新日期：2006-03-01 00:00:00

abstract：:The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones isolated from a HeLa cell cDNA library and confirmed by a combination of comparison to expressed sequence tags, microsequencing of purified protein, and in vitro transcription and trans...

journal_title：Journal of cell science

authors： Singleton DR,Wu TT,Castle JD

更新日期：1997-09-01 00:00:00

abstract：:Organelles such as neuropeptide-containing dense-core vesicles (DCVs) and mitochondria travel down axons to supply synaptic boutons. DCV distribution among en passant boutons in small axonal arbors is mediated by circulation with bidirectional capture. However, it is not known how organelles are distributed in extensi...

journal_title：Journal of cell science

authors： Tao J,Bulgari D,Deitcher DL,Levitan ES

更新日期：2017-08-01 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but ...

journal_title：Journal of cell science

authors： Legal T,Hayward D,Gluszek-Kustusz A,Blackburn EA,Spanos C,Rappsilber J,Gruneberg U,Welburn JPI

更新日期：2020-08-25 00:00:00

abstract：:Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...

journal_title：Journal of cell science

authors： McCaig CD

更新日期：1990-04-01 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:Traction forces increase after microtubule depolymerization; however, the signaling mechanisms underlying this, in particular the dependence upon myosin II, remain unclear. We investigated the mechanism of traction force increase after nocodazole-induced microtubule depolymerization by applying traction force microsco...

journal_title：Journal of cell science

authors： Rape A,Guo WH,Wang YL

更新日期：2011-12-15 00:00:00

abstract：:Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the beta-oxidation of fatty acids. Characterization of their properties and behavior has progressed rather slowly. However, during ...

journal_title：Journal of cell science

authors： van der Zand A,Braakman I,Geuze HJ,Tabak HF

更新日期：2006-03-15 00:00:00

abstract：:Endothelial cells are known to undergo transitions in cell shape during long-term culture. Thus, the assumption that the separate phenotypes of microvascular endothelial cells (MVEC) recently isolated from bovine corpus luteum represent constitutively different cell strains cannot automatically be made. For this reaso...

journal_title：Journal of cell science

authors： Fenyves AM,Behrens J,Spanel-Borowski K

更新日期：1993-11-01 00:00:00

abstract：:Previous investigators have examined mitochondrial DNA (mtDNA) in the electron microscope (EM) after extraction from mitochondria and rotary shadowing. We have observed mtDNA in situ by the osmiumammine procedure for specific staining of DNA in the EM. The procedure was modified to improve the regularity of the staini...

journal_title：Journal of cell science

authors： Liu DF,el-Alfy M,Leblond CP

更新日期：1992-04-01 00:00:00

abstract：:Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was subcloned into GFP expression vectors to generate Rab21 and Rab21 mutants defective in either GTP hydrolysis (Rab21 Q78L) or bi...

journal_title：Journal of cell science

authors： Simpson JC,Griffiths G,Wessling-Resnick M,Fransen JA,Bennett H,Jones AT

更新日期：2004-12-15 00:00:00

abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...

journal_title：Journal of cell science

authors： Song K,Gras C,Capin G,Gimber N,Lehmann M,Mohd S,Puchkov D,Rödiger M,Wilhelmi I,Daumke O,Schmoranzer J,Schürmann A,Krauss M

更新日期：2019-02-01 00:00:00

abstract：:Cytoplasm of Paramecium caudatum syngen 3 in the period of sexual immaturity was effective in inducing immaturity when injected into mating-reactive cells of other syngens of P. caudatum. When immature cytoplasm of P. caudatum was injected into mature cells of P. multimicronucleatum, immaturity was also induced in the...

journal_title：Journal of cell science

authors： Miwa I

更新日期：1979-04-01 00:00:00

abstract：:Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residu...

journal_title：Journal of cell science

authors： Hayashi K,Yonemura S,Matsui T,Tsukita S

更新日期：1999-04-01 00:00:00

abstract：:Heat shock transcription factor 1 (HSF1) regulates the expression of a wide array of genes, controls the expression of heat shock proteins (HSPs) as well as cell growth. Although acute depletion of HSF1 induces cellular senescence, the underlying mechanisms are poorly understood. Here, we report that HSF1 depletion-in...

journal_title：Journal of cell science

authors： Oda T,Sekimoto T,Kurashima K,Fujimoto M,Nakai A,Yamashita T

更新日期：2018-05-08 00:00:00

abstract：:The assembly of an organism requires the interaction between different layers of cells, in many cases via an extracellular matrix. In the developing Drosophila larva, muscles attach in an integrin-dependent manner to the epidermis, via a specialized extracellular matrix called tendon matrix. Tiggrin, a tendon matrix i...

journal_title：Journal of cell science

authors： Martin-Bermudo MD,Brown NH

更新日期：2000-11-01 00:00:00

abstract：:Glomerular mesangial cells (MCs) are central to the pathogenesis of progressive glomeruli-associated renal diseases. However, molecular mechanisms underlying changes in MC functions still remain poorly understood. Here, we show that in MCs, the urokinase-type plasminogen activator (uPA) induces, via its specific recep...

journal_title：Journal of cell science

authors： Shushakova N,Tkachuk N,Dangers M,Tkachuk S,Park JK,Hashimoto K,Haller H,Dumler I

更新日期：2005-06-15 00:00:00

abstract：:Tie2-promoter-mediated loss of peroxisome proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of PPARγ in PMVE...

journal_title：Journal of cell science

authors： Vattulainen-Collanus S,Akinrinade O,Li M,Koskenvuo M,Li CG,Rao SP,de Jesus Perez V,Yuan K,Sawada H,Koskenvuo JW,Alvira C,Rabinovitch M,Alastalo TP

更新日期：2016-02-15 00:00:00

abstract：:Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca(2+) can rapidly erect large membrane boundaries. These might be used as a 'patch' for resealing plasma membrane disruptions. Three central predictions of this 'patch' hypothesis are here established in sea urchin eggs. First, we show that surface markers fo...

journal_title：Journal of cell science

authors： McNeil PL,Vogel SS,Miyake K,Terasaki M

更新日期：2000-06-01 00:00:00

abstract：:Wnts are glycan- and lipid-modified morphogens that are important for cellular responses, but how Wnts are secreted in polarized epithelial cells remains unclear. Although Wntless (Wls) has been shown to interact with Wnts and support their secretion, the role of Wls in the sorting of Wnts to the final destination in ...

journal_title：Journal of cell science

authors： Yamamoto H,Awada C,Hanaki H,Sakane H,Tsujimoto I,Takahashi Y,Takao T,Kikuchi A

更新日期：2013-07-01 00:00:00