Abstract:
:Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles. LETS protein was detected by lactoperoxidase-catalysed radioiodination and microfilament bundles were visualized by indirect immunofluorescence with antibodies directed against actin or myosin. Hybrids which exhibited normal growth control characteristics had high levels of LETS protein and extensive microfilament bundles. Neoplastic transformation appears to be suppressed in these hybrids. Hybrids which had the growth control characteristics typical of transformed cells had reduced or zero levels of LETS protein and few microfilament bundles. These results confirm previous studies on the expression of the transformed phenotype in these hybrids and demonstrate that there is a good correlation between normal growth control and the presence of LETS protein and microfilament bundles. However, the changes in cell surface LETS protein and in the organization of microfilament bundles often appeared to be quantitative reductions rather than all-or-none effects. The magnitude of the alterations in the levels of LETS protein and in the organization of microfilaments appeared to correlate with the range of transformed characteristics exhibited by the cells. One transformed hybrid in particular, selected for growth in agar, had some surface LETS protein, some microfilament bundles and retained density-dependent inhibition of growth.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Marshall CJ,Humphryes KC,Pollack REsubject
Has Abstractpub_date
1978-10-01 00:00:00pages
191-204eissn
0021-9533issn
1477-9137journal_volume
33pub_type
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216101
更新日期:2018-07-09 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1986-09-01 00:00:00
abstract::The novel protein Nowa was identified in nematocysts, explosive organelles of Hydra, jellyfish, corals and other CNIDARIA: Biogenesis of these organelles is complex and involves assembly of proteins inside a post-Golgi vesicle to form a double-layered capsule with a long tubule. Nowa is the major component of the oute...
journal_title:Journal of cell science
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更新日期:2002-10-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.243071
更新日期:2020-06-16 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091124
更新日期:2012-03-01 00:00:00
abstract::Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.073080
更新日期:2011-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.169011
更新日期:2016-02-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.097956
更新日期:2012-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.033233
更新日期:2008-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02510
更新日期:2005-08-15 00:00:00
abstract::Quantitative studies on the adhesive properties of transformed cells have yielded inconclusive and sometimes contradictory results. The present investigation has examined adhesive interactions between normal human fibroblasts, established as well as virus-transformed animal cell lines, and human tumour-derived cell li...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::The protozoan parasite Giardia lamblia has the unusual morphology of bearing two equal-sized nuclei. This organism probably represents the earliest diverging lineage of eukaryotes, suggesting that its biological tactics may be transitional. To begin to understand the role played by the two equal-sized nuclei in this o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The attachment of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an important initial event in the acute inflammatory reaction. Experiments were carried out to examine some of the parameters of this adhesive interaction and the extent to which the substrate (endothelium) plays a role ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::Calmodulin was localized in the small intestine of Necturus maculosa at both the light- and electron-microscopic levels utilizing an affinity-purified, monospecific antibody and immunoperoxidase cytochemistry. The microvilli and basolateral membranes of the epithelium were highly reactive, the cytoplasm was moderately...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2014-04-15 00:00:00