Abstract：
:The attachment of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an important initial event in the acute inflammatory reaction. Experiments were carried out to examine some of the parameters of this adhesive interaction and the extent to which the substrate (endothelium) plays a role in controlling the process. Using a monolayer collection assay for adhesion, we found a requirement for divalent cations. Mn2+ produces maximal adhesion followed by Zn2+ greater than Ni2+ greater than Mg2+ greater than Ba2+ greater than Ca2+. Pretreatment of either the endothelium of PMN with chemotactic agents (zymosan-activated serum, a bacterial filtrate, C5a and formyl-methionyl-leucyl-phenylalanine) causes an increase in the attachment of PMN to endothelial monolayers. Modulations of net surface charge, as measured by laser Doppler electrophoresis, are not correlated to alterations in adhesion. Mn2+, which produces maximal adhesion, reduces the net surface charge to the same extent as Ca2+, which has the smallest effect on adhesion. Similarly, reductions in net surface charge are not responsible for the increases in adhesion due to the chemotactic agents because there are no differences in electrophoretic mobilities between cells treated with either chemotactic agents or their appropriate controls. Scatchard analysis of the binding of the chemotactic tripeptide, formyl-methionyl-leucyl-phenylalanine, indicates the presence of high-affinity binding sites on the surface of the endothelium. The results suggest a role for the endothelium in mediating the adhesive interaction with the PMN.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Hoover RL,Folger R,Haering WA,Ware BR,Karnovsky MJsubject
Has Abstractpub_date
1980-10-01 00:00:00pages
73-86eissn
0021-9533issn
1477-9137journal_volume
45pub_type
杂志文章abstract：:In a previous study we demonstrated that a homeobox peptide corresponding to the 60 amino acid long DNA-binding region of the Drosophila antennapedia homeo-protein was capable of crossing the plasma membrane of cells in culture. This finding has led us to investigate whether chimeric molecules encompassing the homeobo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:A total arrest of cell membrane movement occurred after incubation for 2 h of cultured fibroblasts with the polycation DEAE-dextran. The immobilized cells resumed normal membrane undulations after exposure for 5 min to the polyanion dextran-sulphate. The influence of DEAE-dextran on the electrophoretic mobility, which...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:Intracellular signal transduction occurs through cascades of reactions involving dozens of proteins that transmit signals from the cell surface, through a crowded cellular environment filled with organelles and a filamentous cytoskeleton, to specific targets. Numerous signaling molecules are immobilized or transiently...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01122
更新日期：2004-06-01 00:00:00
abstract：:Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (mi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.164004
更新日期：2015-02-15 00:00:00
abstract：:An intriguing question in cell biology is what targets proteins to, and regulates their translocation between, specific cellular locations. Here we report that the polybasic nuclear localization sequence (NLS) required for nuclear entry of the adapter protein and candidate human obesity gene product SH2B1β, also local...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.078949
更新日期：2011-05-01 00:00:00
abstract：:The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.091124
更新日期：2012-03-01 00:00:00
abstract：:In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.216259
更新日期：2018-08-03 00:00:00
abstract：:The mechanisms underlying stromal cell supportive functions are incompletely understood but probably implicate a mixture of cytokines, matrix components and cell adhesion molecules. Skeletal muscle uses recruited macrophages to support post-injury regeneration. We and others have previously shown that macrophages secr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02988
更新日期：2006-06-15 00:00:00
abstract：:Motile eukaryotic flagella beat through coordinated activity of dynein motor proteins; however, the mechanisms of dynein coordination and regulation are incompletely understood. The inner dynein arm (IDA) f complex (also known as the I1 complex), and the tether and tether head (T/TH) complex are thought to be key regu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.239855
更新日期：2020-01-23 00:00:00
abstract：:New concepts in cell organization emerged in a medieval castle during a snowy week in January 2017 in the middle of the Austrian Alps. The occasion was the 10th Annaberg EMBO workshop in Goldegg am See; organized by Gabriele Seethaler, Catherine Rabouille and Marino Zerial. There were 95 participants, including many w...
journal_title：Journal of cell science
pub_type：
doi：10.1242/jcs.206219
更新日期：2017-07-15 00:00:00
abstract：:Earlier reports have indicated that the pollen of Eucalyptus is mechanically robust and unusually resistant to the osmotic stress imposed by immersion in water. We have investigated some of the features of the germination mechanism in the pollen of E. rhodantha with a view to clarifying the role of pollen-wall special...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-02-01 00:00:00
abstract：:The ret finger protein (rfp) is a member of the B box zinc finger gene family which possesses a tripartite motif consisting of a RING finger, B box finger, and a coiled-coil domain. Rfp is expressed at specific stages of spermatogenesis and in various adult mouse and human tissues. It becomes oncogenic when the tripar...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Non-coding RNAs (ncRNAs) are nucleotide sequences that are known to assume regulatory roles previously thought to be reserved for proteins. Their functions include the regulation of protein activity and localization and the organization of subcellular structures. Sequencing studies have now identified thousands of ncR...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.246744
更新日期：2020-11-05 00:00:00
abstract：:Delayed wound healing caused by inefficient re-epithelialization underlines chronic skin lesions such as those found in diabetes. The dioxin receptor (AhR) modulates cell plasticity and migration and its activation by occupational polycyclic aromatic hydrocarbons (PAHs) results in severe skin lesions such as contact h...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.047274
更新日期：2009-06-01 00:00:00
abstract：:In plants, many nucleus-encoded proteins are targeted to both mitochondria and plastids, and this process is generally mediated by ambiguous N-terminal targeting sequences that are recognized by receptors on both organelles. In many algae, however, plastids were acquired by secondarily engulfing green or red algae, wh...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.116533
更新日期：2012-12-15 00:00:00
abstract：:Immediately following embryonic cleavage, the cells of Astrephomene have four equal-sized basal bodies, two of which are connected by a striated distal fibre and two striated proximal fibres. The four microtubular rootlets, which alternate between having 3/1 and 2 members, are arranged cruciately. The two basal bodies...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
abstract：:Several examples of direct interactions between helicases and topoisomerases have recently been described. The data suggest a possible cooperation between these enzymes in major DNA events such as the progression of a replication fork, segregation of newly replicated chromosomes, disruption of nucleosomal structure, D...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：1997-06-01 00:00:00
abstract：:Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.098871
更新日期：2012-10-15 00:00:00
abstract：:Rapid endocytosis of lipoprotein receptors involves NPxY signals contained in their cytoplasmic tails. Several proteins, including ARH and Dab2, can bind these sequences, but their importance for endocytosis may vary in different cell types. The lipoprotein receptor megalin is expressed in the visceral endoderm (VE), ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02650
更新日期：2005-11-15 00:00:00
abstract：:During the last phase of oogenesis in Drosophila, nurse cell cytoplasm can be seen to be streaming into the growing oocyte when visualized in time-lapse films. This process can be reversibly inhibited by cytochalasins. The distribution of F-actin filaments in the nurse cells has been studied by staining with rhodamine...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:Glutathione (GSH) levels progressively decline during aging and in neurodegenerative disorders. However, the contribution of such event in mediating neuronal cell death is still uncertain. In this report, we show that, in neuroblastoma cells as well as in primary mouse cortical neurons, GSH decrease, induced by buthio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.077149
更新日期：2011-04-01 00:00:00
abstract：:Prion diseases are fatal neurodegenerative diseases characterised by the accumulation of misfolded prion protein (PrP(Sc)) in the brain. They are caused by the templated misfolding of normal cellular protein, PrP(C), by PrP(Sc). We have recently generated a unique cell system in which epitope-tagged PrP(C) competent t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.120477
更新日期：2013-08-15 00:00:00
abstract：:Shape changes have been determined in human blood lymphocytes stimulated with OAG, diC8, PMA, colchicine or the hexapeptide fNLPNTL in short-term assays (30 min). Distinct types of shape-change responses were observed. Colchicine was active in generating a relatively small proportion of polarized lymphocytes (front-ta...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Mitotic spindle assembly in animal cells is orchestrated by a chromosome-dependent pathway that directs microtubule stabilization. RanGTP generated at chromosomes releases spindle assembly factors from inhibitory complexes with importins, the nuclear transport factors that facilitate protein import into the nucleus du...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126854
更新日期：2013-08-01 00:00:00
abstract：:The 5T4 oncofoetal antigen is a 72 kDa glycoprotein defined by a monoclonal antibody raised against human placental trophoblast and is expressed in many different carcinomas but detected only at low levels in some normal epithelia. Immunohistochemical analysis of the patterns of expression in colorectal carcinomas has...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.123612
更新日期：2013-08-15 00:00:00
abstract：:Prox1 plays pivotal roles during embryonic lymphatic development and maintenance of adult lymphatic systems by modulating the expression of various lymphatic endothelial cell (LEC) markers, such as vascular endothelial growth factor receptor 3 (VEGFR3). However, the molecular mechanisms by which Prox1 transactivates i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.083998
更新日期：2011-08-15 00:00:00
abstract：:We have identified and characterized two proteins in rat brain that bind to the neuron-specific tropomyosin isoform, TMBr3. The two proteins were identified by blot overlay assay, in which the proteins immobilized on the membrane were probed by epitope-tagged TMBr3, followed by detection with anti-epitope antibody. We...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:When they are fixed with glutaraldehyde in the presence of calcium ions, the plasma membranes of Beroe giant smooth muscle fibres display micropapillae filled with an electron-dense deposit. After freeze-fracturing of fixed tissue, the micropapillae are still present, therefore their shape and size are determined duri...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:In Drosophila, telomere-capping proteins have the remarkable capacity to recognize chromosome ends in a sequence-independent manner. This epigenetic protection is essential to prevent catastrophic ligations of chromosome extremities. Interestingly, capping proteins occupy a large telomere chromatin domain of several k...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.167825
更新日期：2015-05-15 00:00:00
