Abstract:
:Mutation of the LMNA gene, encoding nuclear lamin A and lamin C (hereafter lamin A/C), is a common cause of familial dilated cardiomyopathy (DCM). Among Finnish DCM patients, the founder mutation c.427T>C (p.S143P) is the most frequently reported genetic variant. Here, we show that p.S143P lamin A/C is more nucleoplasmic and soluble than wild-type lamin A/C and accumulates into large intranuclear aggregates in a fraction of cultured patient fibroblasts as well as in cells ectopically expressing either FLAG- or GFP-tagged p.S143P lamin A. In fluorescence loss in photobleaching (FLIP) experiments, non-aggregated EGFP-tagged p.S143P lamin A was significantly more dynamic. In in vitro association studies, p.S143P lamin A failed to form appropriate filament structures but instead assembled into disorganized aggregates similar to those observed in patient cell nuclei. A whole-genome expression analysis revealed an elevated unfolded protein response (UPR) in cells expressing p.S143P lamin A/C. Additional endoplasmic reticulum (ER) stress induced by tunicamycin reduced the viability of cells expressing mutant lamin further. In summary, p.S143P lamin A/C affects normal lamina structure and influences the cellular stress response, homeostasis and viability.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
West G,Gullmets J,Virtanen L,Li SP,Keinänen A,Shimi T,Mauermann M,Heliö T,Kaartinen M,Ollila L,Kuusisto J,Eriksson JE,Goldman RD,Herrmann H,Taimen Pdoi
10.1242/jcs.184150subject
Has Abstractpub_date
2016-07-15 00:00:00pages
2732-43issue
14eissn
0021-9533issn
1477-9137pii
jcs.184150journal_volume
129pub_type
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